A6LEJ3 (EFG_PARD8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor G
Short name=EF-G

Gene names

Name:	fusA
Ordered Locus Names:	BDI_2382

Organism

Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / NCTC 11152) [Complete proteome] [HAMAP]

Taxonomic identifier

435591 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Porphyromonadaceae › Parabacteroides

Protein attributes

Sequence length	708 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity. HAMAP MF_00054_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00054_B.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 708	708	Elongation factor G HAMAP MF_00054_B		PRO_0000335851
Regions
	Nucleotide binding	18 – 25	8	GTP By similarity
	Nucleotide binding	86 – 90	5	GTP By similarity
	Nucleotide binding	140 – 143	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6LEJ3 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: 3D944D7BE0DDC729	FASTA	708	78,377
	10 20 30 40 50 60 MANNDKQLMF TRNIGIMAHI DAGKTTTSER ILFYTGLTHK IGETHDGTAT MDWMAQEQER 70 80 90 100 110 120 GITITSAATT TFWNYLGNRY KINLIDTPGH VDFTVEVERS LRVLDGAVAT FCAVGGVEPQ 130 140 150 160 170 180 SETVWRQADK YNVPRIGYVN KMDRSGANYY EVVRQLKDVL GANPCPIQIP IGAEETFKGV 190 200 210 220 230 240 VDLIKMKAIF WHDETMGAEY SVEEIPADLQ AEAEEWRDKM LEALAECDDA IMEKYFDDPS 250 260 270 280 290 300 TITEEEIMVA IRKGTLAMQI NPMTCGSSFK NKGVQTLLDA VCAFLPSPED TPAIEGTDPS 310 320 330 340 350 360 DPDKIITRKP LFEEPLTALA FKIATDPYVG RLCFFRVYAG SINAGSYVLN TRSGKKERIS 370 380 390 400 410 420 RLFQMHSNKQ NPMEVIGCGD IGAGVGFKDI RTGDTLCDEN HPITLESMEF PEPVIGIAVE 430 440 450 460 470 480 PKTQKDMDKL GMGLAKLAEE DPTFRVQTNE ETGQTVISGM GELHLDIIID RLRREFKVEC 490 500 510 520 530 540 NQGRPQVTYK EAITQPVELR EVYKKQSGGR GKFADIIVRM EPADESFEGT LQFIDEVKGG 550 560 570 580 590 600 NIPKEFIPSV QKGFEKAMKN GVLAGYPLDK LKVTLIDGSF HPVDSDQLSF EIAAIQAFKN 610 620 630 640 650 660 ASAKAGPVLM EPIMQMEVVT PEESMGDVIG DLNKRRGQVE GMETSRTGAR IVKAKVPLAE 670 680 690 700 TFGYVTALRT ITSGRATSSM QFSHYAQVSS SIAKQVLTEV QGRVDLIK

References

[1]

"Evolution of symbiotic bacteria in the distal human intestine." Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C., Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H., Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K., Knight R.D., Gordon J.I. PLoS Biol. 5:1574-1586(2007) [PubMed: 17579514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8503 / DSM 20701 / NCTC 11152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000140 Genomic DNA. Translation: ABR44107.1.
RefSeq	YP_001303729.1. NC_009615.1.
3D structure databases
ProteinModelPortal	A6LEJ3.
ModBase	Search...
Protein-protein interaction databases
STRING	A6LEJ3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5307531.
GenomeReviews	Gene locus BDI_2382 in contig CP000140_GR.
KEGG	pdi:BDI_2382.
PATRIC	22847984. VBIParDis29947_2367.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0480.
HOGENOM	HBG737692.
OMA	CNEWREK.
ProtClustDB	PRK12739.
Enzyme and pathway databases
BioCyc	PDIS435591:BDI_2382-MONOMER.
Family and domain databases
HAMAP	MF_00054_B. EF-G_EF-2_B. [Tree]
InterPro	IPR009022. Elongation_fac_G/III/V. IPR000795. ProtSyn_GTP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005225. Small_GTP-bd_dom. IPR004540. Transl_elong_EFG/EF2. IPR000640. Transl_elong_EFG/EF2_C. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.30.70.240. Transl_elong_EFG/EF2_C. 1 hit.
KO	K02355.
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. SM00889. EFG_IV. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00484. EF-G. 1 hit. TIGR00231. Small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFG_PARD8

Accession

Primary (citable) accession number: A6LEJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 24, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program