A6LDP0 (ILVD_PARD8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	BDI_2073

Organism

Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / NCTC 11152) [Complete proteome] [HAMAP]

Taxonomic identifier

435591 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Porphyromonadaceae › Parabacteroides

Protein attributes

Sequence length	611 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 611	611	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000001023
Sites
	Metal binding	123	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	192	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A6LDP0 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: 9AA028BA2E76E7D0	FASTA	611	65,859
	10 20 30 40 50 60 MKNPLRSSFS TEGRRMAGAR ALWRANGMKE EQFGKPIIAI VNSFTQFVPG HTHLHEIGQL 70 80 90 100 110 120 VKAEIEKQGC FAAEFNTIAI DDGIAMGHDG MLYSLPSRDI IADSVEYMVN AHKADAMVCI 130 140 150 160 170 180 SNCDKITPGM LMASMRLNIP TVFVSGGPME AGELDGRHLD LIDAMIESAD TSVSDERIEQ 190 200 210 220 230 240 VERHACPGCG CCSGMFTANS MNCLNEAIGL ALPGNGTIVA THKNRIQLFR DAAKQIVENA 250 260 270 280 290 300 YKYYRDGDDS VLPRNIATRQ AFLNAMSLDI AMGGSTNTVL HLLAVAQEAG ADFHMEDIDM 310 320 330 340 350 360 LSRKTPCLCK VAPNTHTYHV QDVNRAGGIL GIMNELMKAG LVDGSTRRAD GLTLAEAVDK 370 380 390 400 410 420 YAVTSPNVTE EAIRKYKSAP AHRFSIQMGS QESYYKELDT DRAEGCIRDV EHAYSKDGGL 430 440 450 460 470 480 AVLRGNIALD GCVVKTAGVD ESIWKFSGPA KVFDSQDAAC EGILGGKVVS GDVVVITYEG 490 500 510 520 530 540 PKGGPGMQEM LYPTSYIKSR HLGKECALIT DGRFSGGTSG LSIGHISPEA ASGGAIGLVR 550 560 570 580 590 600 DGDIIEINIP ERSINVRLSD EELAERRKAE EARGKKAFTP PTRQREVSKA LRAYGKMVSS 610 ADKGGVRIVE D

References

[1]

"Evolution of symbiotic bacteria in the distal human intestine." Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C., Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H., Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K., Knight R.D., Gordon J.I. PLoS Biol. 5:1574-1586(2007) [PubMed: 17579514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8503 / DSM 20701 / NCTC 11152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000140 Genomic DNA. Translation: ABR43804.1.
RefSeq	YP_001303426.1. NC_009615.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6LDP0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5307222.
GenomeReviews	Gene locus BDI_2073 in contig CP000140_GR.
KEGG	pdi:BDI_2073.
PATRIC	22847352. VBIParDis29947_2057.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK12448.
Enzyme and pathway databases
BioCyc	PDIS435591:BDI_2073-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_PARD8

Accession

Primary (citable) accession number: A6LDP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 24, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program