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A6LD84 (BIOB_PARD8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:BDI_1913
OrganismParabacteroides distasonis (strain ATCC 8503 / DSM 20701 / NCTC 11152) [Complete proteome] [HAMAP]
Taxonomic identifier435591 [NCBI]
Taxonomic lineageBacteriaBacteroidetesBacteroidiaBacteroidalesPorphyromonadaceaeParabacteroides

Protein attributes

Sequence length327 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 327327Biotin synthase HAMAP-Rule MF_01694
PRO_0000381514

Sites

Metal binding621Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding661Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding691Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1381Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1981Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2681Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A6LD84 [UniParc].

Last modified July 24, 2007. Version 1.
Checksum: 2ADB15A9CC5D7088

FASTA32736,871
        10         20         30         40         50         60 
MTIEEAKQHI LEGGKITEEQ ALSLANHPDK EALYEAAHQI TRHFMGNKFD TCSIINAKSG 

        70         80         90        100        110        120 
NCSEDCKWCA QSGHYKTLVN LYPLLPAKEC VYHAVYNRKQ GIRRFALVTS GKRVSDKELE 

       130        140        150        160        170        180 
QITDTIRQIK RQSDIKCCAS MGLLTRSQLQ SLYDSGVENY HCNIETAPSY FRQLCSTHTI 

       190        200        210        220        230        240 
EQKMETIHTA REIGFRICCG GIIGMGETMK ERIEMACFLQ KEGVLSIPLN LLQPIPGTPM 

       250        260        270        280        290        300 
ENTQILEEEE WLTTIALFRL INPNAFLRFS GGRAQLSEVT QRKSLHIGIN SAIIGDLLTT 

       310        320 
IGSKVEEDKV LFTSEGYSLT ENTDWEK 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000140 Genomic DNA. Translation: ABR43648.1.
RefSeqYP_001303270.1. NC_009615.1.

3D structure databases

ProteinModelPortalA6LD84.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING435591.BDI_1913.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR43648; ABR43648; BDI_1913.
GeneID5307063.
KEGGpdi:BDI_1913.
PATRIC22847028. VBIParDis29947_1897.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMADETQALC.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycPDIS435591:GCNH-1908-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_PARD8
AccessionPrimary (citable) accession number: A6LD84
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 24, 2007
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways