A6LA57 (DAPA_PARD8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	BDI_0801

Organism

Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / NCTC 11152) [Complete proteome] [HAMAP]

Taxonomic identifier

435591 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Porphyromonadaceae › Parabacteroides

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_1000050235
Regions
	Region	52 – 53	2	Pyruvate binding By similarity
Sites
	Active site	166	1	Schiff-base intermediate with substrate By similarity
	Binding site	110	1	Pyruvate By similarity
	Site	137	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6LA57 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: BCB281BB2C1A43D1	FASTA	297	32,712
	10 20 30 40 50 60 MADINLKGMG VALITPFKED ESVDYEALGK LVDYQVQNGT DYLVVLGTTA ETPTLTEEEK 70 80 90 100 110 120 KNIISLVVTR VRGRIPIVLG VGGNCTRSVV EKLKTDNFEG IDAILSVVPY YNKPSQEGIY 130 140 150 160 170 180 QHYKAIANAT HLPIVLYNVP GRTGVNMTAE TTLRIAREFD NVIAVKEASG NITQMDDIIK 190 200 210 220 230 240 NKPARFNVIS GDDGITFPLM TLGAVGVISV IGNAFPREFS RMVRLALAGD YDSARTIHHS 250 260 270 280 290 FTELFSLLFV DGNPAGAKSM LNMMGFIENK LRLPLVPTRI VTYEKIREVL RQLSIKC

References

[1]

"Evolution of symbiotic bacteria in the distal human intestine." Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C., Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H., Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K., Knight R.D., Gordon J.I. PLoS Biol. 5:1574-1586(2007) [PubMed: 17579514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8503 / DSM 20701 / NCTC 11152.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000140 Genomic DNA. Translation: ABR42571.1.
RefSeq	YP_001302193.1. NC_009615.1.
3D structure databases
ProteinModelPortal	A6LA57.
ModBase	Search...
Protein-protein interaction databases
STRING	A6LA57.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5305955.
GenomeReviews	Gene locus BDI_0801 in contig CP000140_GR.
KEGG	pdi:BDI_0801.
PATRIC	22844744. VBIParDis29947_0781.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0329.
HOGENOM	HBG358848.
OMA	CEMEDSN.
ProtClustDB	PRK03170.
Enzyme and pathway databases
BioCyc	PDIS435591:BDI_0801-MONOMER.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR002220. Dihydrodipicolinate_synth-like. IPR020625. Dihydrodipicolinate_synth_AS. IPR005263. Dihydrodipicolinate_synth_DapA. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K01714.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PIRSF	PIRSF001365. DHDPS. 1 hit.
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. DapA. 1 hit.
PROSITE	PS00665. DHDPS_1. False negative. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_PARD8

Accession

Primary (citable) accession number: A6LA57

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 24, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program