Reviewed, UniProtKB/Swiss-Prot A6L890 (NRFA_PARD8)

Names and origin

Protein names

Recommended name:
    Cytochrome c-552
    EC=1.7.2.2
Alternative name(s):
    Ammonia-forming cytochrome c nitrite reductase
      Short name=Cytochrome c nitrite reductase

Gene names

Name:	nrfA
Ordered Locus Names:	BDI_0109

Organism

Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / NCTC 11152) [Complete proteome] [HAMAP]

Taxonomic identifier

435591 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Porphyromonadaceae › Parabacteroides

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays a role in nitrite reduction By similarity.
Catalytic activity	NH(3) + 2 H(2)O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H(+).
Cofactor	Binds 1 calcium ion per monomer By similarity. Binds 5 heme groups covalently per monomer By similarity.
Pathway	Nitrogen metabolism; nitrate reduction (assimilation).
Subcellular location	PeriplasmBy similarity.
Sequence similarities	Belongs to the cytochrome c-552 family.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Periplasm
Domain	Repeat Signal
Ligand	Calcium Heme Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW nitrogen compound metabolic process Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: HAMAP
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitrite reductase (cytochrome, ammonia-forming) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 494	463	Cytochrome c-552		PRO_1000065804
Sites
	Metal binding	116	1	Iron (heme 3 axial ligand) By similarity
	Metal binding	148	1	Iron (heme 1 axial ligand) By similarity
	Metal binding	186	1	Iron (heme 2 axial ligand) By similarity
	Metal binding	228	1	Iron (heme 3 axial ligand) By similarity
	Metal binding	230	1	Calcium By similarity
	Metal binding	231	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	276	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	278	1	Calcium By similarity
	Metal binding	290	1	Iron (heme 5 axial ligand) By similarity
	Metal binding	301	1	Iron (heme 4 axial ligand) By similarity
	Metal binding	315	1	Iron (heme 2 axial ligand) By similarity
	Metal binding	332	1	Iron (heme 5 axial ligand) By similarity
	Metal binding	407	1	Iron (heme 4 axial ligand) By similarity
	Binding site	144	1	Heme 1 (covalent) By similarity
	Binding site	147	1	Heme 1 (covalent) By similarity
	Binding site	182	1	Heme 2 (covalent) By similarity
	Binding site	185	1	Heme 2 (covalent) By similarity
	Binding site	224	1	Heme 3 (covalent) By similarity
	Binding site	227	1	Heme 3 (covalent) By similarity
	Binding site	231	1	Substrate By similarity
	Binding site	279	1	Substrate By similarity
	Binding site	297	1	Heme 4 (covalent) By similarity
	Binding site	300	1	Heme 4 (covalent) By similarity
	Binding site	328	1	Heme 5 (covalent) By similarity
	Binding site	331	1	Heme 5 (covalent) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6L890-1 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: 58ECCCF0E077EF53	FASTA	494	56,145
	10 20 30 40 50 60 MEKKLKSWQG WLLFGGTMVV VFVLGMIAAS VNERHAEVTS VMNNKKTEIT GIEARNDKFE 70 80 90 100 110 120 SNYPREYQTW EATADTSFKS LYNGNQAVDV LEARPEMVIL WAGYAFSKDY STPRGHMHAI 130 140 150 160 170 180 EDMRNTLRVG APMTENEGPQ PATCWTCKSP DVPRMMQAMG VDNFYKGKWA SLGKEIVNPI 190 200 210 220 230 240 GCADCHEPEN MNLHISRPAL IEAFQRQGKD ITKATQQEMR SLVCAQCHVE YYFKGDGKYL 250 260 270 280 290 300 TFPWDKGSTV EDMEAYYDEA GFADYTHKLS RAPILKAQHP DYEISQMGIH AQRGVSCADC 310 320 330 340 350 360 HMPYKSEGGV KYSDHHIQSP LAMIDRTCQV CHRESEETLR NNVYERQNKA NEMRNRLETE 370 380 390 400 410 420 LAKAHVEAKF AWDKGATEDQ MKDVLKLIRQ AQWRWDFGVA SHGGAFHAPQ EIQRILGNGL 430 440 450 460 470 480 DKAMQARLAT AKVLAKLGYT DDVPMPDFST KEKAQQYIGL DMAAERTAKE KFLNTIVPQW 490 MKEAQENNRL AKNI

References

[1]

"Evolution of symbiotic bacteria in the distal human intestine." Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C., Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H., Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K., Knight R.D., Gordon J.I. PLoS Biol. 5:1574-1586(2007) [PubMed: 17579514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000140 Genomic DNA. Translation: ABR41904.1.
RefSeq	YP_001301526.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5305200.
GenomeReviews	Gene locus BDI_0109 in contig CP000140_GR.
KEGG	pdi:BDI_0109.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01182. [Tree]
InterPro	IPR003321. Cyt_c552. IPR011031. Multihaem_cyt. [Graphical view]
Pfam	PF02335. Cytochrom_C552. 1 hit. [Graphical view]
PIRSF	PIRSF000243. Cyt_c552. 1 hit.
PROSITE	PS51008. MULTIHEME_CYTC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NRFA_PARD8

Accession

Primary (citable) accession number: A6L890

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	July 24, 2007
Last modified:	November 4, 2008
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)