A6L7J7 (SYT_BACV8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Threonine--tRNA ligase
EC=6.1.1.3

Alternative name(s):
Threonyl-tRNA synthetase
Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	BVU_4059

Organism

Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / NCTC 11154) [Complete proteome] [HAMAP]

Taxonomic identifier

435590 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	646 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00184
Subunit structure	Homodimer By similarity. HAMAP MF_00184
Subcellular location	Cytoplasm HAMAP MF_00184.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 646	646	Threonine--tRNA ligase HAMAP MF_00184		PRO_1000020342
Regions
	Region	242 – 541	300	Catalytic HAMAP MF_00184
Sites
	Metal binding	337	1	Zinc; catalytic By similarity
	Metal binding	388	1	Zinc; catalytic By similarity
	Metal binding	518	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6L7J7 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: D5FE668DF9E49AC7	FASTA	646	74,213
	10 20 30 40 50 60 MIKITFPDGS VREYNEGVTG LQIAESISSR LAQDVLACGV NGETIDLSRP INEDANFVLY 70 80 90 100 110 120 KWEDEQGKHA FWHTSAHLLA EALQELYPGI QFGIGPAIEN GFYYDVDPGE AIIKEADLPA 130 140 150 160 170 180 IEAKMAELAA KKEILVRQSI AKEDALKKFG ERGETYKCEL ISELEDGHIT TYTQGAFTDL 190 200 210 220 230 240 CRGPHLTSTA PIKAIKLLTV AGAYWRGQED RKQMTRIYGI TFPKKKMLDE YLVMLEEAKK 250 260 270 280 290 300 RDHRKIGKEM DLFMFTDMVG KGLPMWLPKG TALRIRLQDF LRRIQARYDY QEVMCPPIGN 310 320 330 340 350 360 KNLYITSGHY AKYGKDSFQP IHTPEEGEEY FLKPMNCPHH CMIYKNSPRS YKDLPLRIAE 370 380 390 400 410 420 FGTVCRYEQS GELHGLTRVR SFTQDDAHLF CRPDQVKEEF LRVMDIINIV FKSMNFENVE 430 440 450 460 470 480 AQISLRDKVN REKYIGSDEN WEKAEQAIIE ACEEKGLTAK VEYGEAAFYG PKLDFMVKDA 490 500 510 520 530 540 IGRRWQLGTI QVDYNLPERF QLEYMGSDNQ KHRPVMIHRA PFGSMERFVA VLIEHTAGKF 550 560 570 580 590 600 PLWLTPDQVA ILPISEKFNE YAEQVKAELR KFDVRAIVDD RNEKIGRKIR DNEMKRIPYM 610 620 630 640 LIVGEKEAEN GEVAVRKQGE GDKGTMKIEE FGKNLAEEVS NMINKW

References

[1]

"Evolution of symbiotic bacteria in the distal human intestine." Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C., Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H., Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K., Knight R.D., Gordon J.I. PLoS Biol. 5:1574-1586(2007) [PubMed: 17579514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8482 / DSM 1447 / NCTC 11154.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000139 Genomic DNA. Translation: ABR41661.1.
RefSeq	YP_001301283.1. NC_009614.1.
3D structure databases
ProteinModelPortal	A6L7J7.
ModBase	Search...
Protein-protein interaction databases
STRING	A6L7J7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5305018.
GenomeReviews	Gene locus BVU_4059 in contig CP000139_GR.
KEGG	bvu:BVU_4059.
NMPDR	fig|435590.6.peg.3773.
PATRIC	21073216. VBIBacVul85104_4169.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0441.
HOGENOM	HBG352811.
OMA	GRKWQLG.
ProtClustDB	PRK00413.
Family and domain databases
HAMAP	MF_00184. Thr_tRNA_synth. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR012675. Beta-grasp_ferredoxin-type. IPR004095. TGS. IPR012676. TGS-like. IPR002320. Thr-tRNA-synth_IIa. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
KO	K01868.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF81271. TGS-like. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00418. ThrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_BACV8

Accession

Primary (citable) accession number: A6L7J7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 24, 2007
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program