Reviewed, UniProtKB/Swiss-Prot A6L5X6 (ISPE_BACV8)

Names and origin

Protein names

Recommended name:
    4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
      Short name=CMK
    EC=2.7.1.148
Alternative name(s):
    4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	BVU_3466

Organism

Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / NCTC 11154) [Complete proteome] [HAMAP]

Taxonomic identifier

435590 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity.
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000007812
Regions
	Nucleotide binding	95 – 105	11	ATP Potential
Sites
	Active site	8	1	By similarity
	Active site	137	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6L5X6-1 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: 89C191BA13C2121A	FASTA	275	30,679
	10 20 30 40 50 60 MITFPNAKIN LGLNIVEKRP DGYHNLETIF YPINLQDALE VTRRENNDKE YTLHISGSPL 70 80 90 100 110 120 EGEPEDNLVV QAYKLLKKDY PGLLPVDIHM YKHIPAGAGL GGGSSDAACM IKLLNDKFSL 130 140 150 160 170 180 GLSTERMEEY AVKLGADCAF FIRNKPVFAT GIGNLFEPVE LSLKGYHIIL IKPDIFVSTR 190 200 210 220 230 240 DAFAEIKPVR PAVSLKEIVK QPMETWKNSM KNDFEDSVFK KFPEIAAIKD ELYDLGAVYA 250 260 270 AMSGSGSSVY GIFKAPIENV EDKFCGCFCR QRALE

References

[1]

"Evolution of symbiotic bacteria in the distal human intestine." Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C., Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H., Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K., Knight R.D., Gordon J.I. PLoS Biol. 5:1574-1586(2007) [PubMed: 17579514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000139 Genomic DNA. Translation: ABR41090.1.
RefSeq	YP_001300712.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6L5X6.
Genome annotation databases
GeneID	5304426.
GenomeReviews	Gene locus BVU_3466 in contig CP000139_GR.
KEGG	bvu:BVU_3466.
NMPDR	fig|435590.6.peg.3232.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VFYPIPL.
Family and domain databases
HAMAP	MF_00061. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. [Graphical view]
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
TIGRFAMs	TIGR00154. ispE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_BACV8

Accession

Primary (citable) accession number: A6L5X6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 24, 2007
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)