A6L5I2 (SYP_BACV8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	BVU_3320

Organism

Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / NCTC 11154) [Complete proteome] [HAMAP]

Taxonomic identifier

435590 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	Proline--tRNA ligase HAMAP MF_01571		PRO_1000069190

Sequences

	Sequence		Length	Mass (Da)
	A6L5I2 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: CE0E381C8555E88C	FASTA	496	56,830
	10 20 30 40 50 60 MAVELKELTK RSENYSQWYN DLVVKADLAE QSPVRGCMVI KPYGYAIWEK MQRQLDDMFK 70 80 90 100 110 120 ETGHVNAYFP LLIPKSYLSR EAEHVEGFAK ECAVVTHYRL KNAEDGSGVI VDPAAKLEEE 130 140 150 160 170 180 LIIRPTSETI IWSTYKNWIN SYRDLPILCN QWANVMRWEM RTRLFLRTAE FLWQEGHTAH 190 200 210 220 230 240 ATREEAEAEA QKMLHVYGDF AEKYMAVPVI KGVKSANERF AGALDTYTIE GLMQDGKALQ 250 260 270 280 290 300 CGTSHFLGQN FAKAFNVQFV DKNNKLDYVW ATSWGVSTRL MGALIMTHSD DNGLVLPPHL 310 320 330 340 350 360 APIQVVIVPI YKNDEMLKKI DAKVEGIVNK LKAMGISVKY DNADNKRPGF KFADYELKGV 370 380 390 400 410 420 PVRLVMGGRD LENNTMEVMR RDTLEKETRS CDGIEEYVQQ LLEDIQNNIY QKALNYRNEH 430 440 450 460 470 480 IVKVDSYDDF KEQIEKGGFI LAHWDGTPET EDRIKEETKA TIRCLPFDAD EESLTPGKCM 490 VTGKPSARRV LFARAY

References

[1]

"Evolution of symbiotic bacteria in the distal human intestine." Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C., Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H., Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K., Knight R.D., Gordon J.I. PLoS Biol. 5:1574-1586(2007) [PubMed: 17579514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8482 / DSM 1447 / NCTC 11154.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000139 Genomic DNA. Translation: ABR40946.1.
RefSeq	YP_001300568.1. NC_009614.1.
3D structure databases
ProteinModelPortal	A6L5I2.
SMR	A6L5I2. Positions 6-496.
ModBase	Search...
Protein-protein interaction databases
STRING	A6L5I2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5304281.
GenomeReviews	Gene locus BVU_3320 in contig CP000139_GR.
KEGG	bvu:BVU_3320.
NMPDR	fig|435590.6.peg.3094.
PATRIC	21071668. VBIBacVul85104_3415.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0442.
HOGENOM	HBG334108.
OMA	KFAEYEL.
ProtClustDB	PRK08661.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR004499. Pro-tRNA-synth_IIa_arc-type. IPR017449. Pro-tRNA_synth_II. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09180. ProRS-C_1. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
SMART	SM00946. ProRS-C_1. 1 hit. [Graphical view]
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. ProS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_BACV8

Accession

Primary (citable) accession number: A6L5I2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	July 24, 2007
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program