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A6L313 (MEND_BACV8) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Short name=SEPHCHC synthase
EC=2.2.1.9
Alternative name(s):
Menaquinone biosynthesis protein MenD
Gene names
Name:menD
Ordered Locus Names:BVU_2418
OrganismBacteroides vulgatus (strain ATCC 8482 / DSM 1447 / NCTC 11154) [Complete proteome] [HAMAP]
Taxonomic identifier435590 [NCBI]
Taxonomic lineageBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides

Protein attributes

Sequence length557 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) By similarity. HAMAP-Rule MF_01659

Catalytic activity

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2. HAMAP-Rule MF_01659

Cofactor

Magnesium or manganese By similarity. HAMAP-Rule MF_01659

Binds 1 thiamine pyrophosphate per subunit By similarity.

Pathway

Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 2/8. HAMAP-Rule MF_01659

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01659

Sequence similarities

Belongs to the TPP enzyme family. MenD subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 5575572-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase HAMAP-Rule MF_01659
PRO_0000341717

Sequences

Sequence LengthMass (Da)Tools
A6L313 [UniParc].

Last modified July 24, 2007. Version 1.
Checksum: 440921E36AAB4DB0

FASTA55762,971
        10         20         30         40         50         60 
MYSDKKNILQ LVALLKAHGV RKIVLCPGSR NAAIVHTLAN IEDFTCYSVT DERSAGFFAI 

        70         80         90        100        110        120 
GLSLQGGGPA AVCCTSGSAL LNLHPAVAEA FYQQVPLIVI SADRPAAWIG QMDGQTLPQP 

       130        140        150        160        170        180 
HVFGTLVKMS VNLPEVHTEE DEWFCNRLIN EAILETTHHG KGPVHINVPI SEPIYRFTAK 

       190        200        210        220        230        240 
TLPEVRVITR YQGLSVYDRD YKELIERLNK YNKRMVVVGQ MNLIYLFEKK YVKPLYKHFA 

       250        260        270        280        290        300 
WLTEHLGNQT IPGIPIKNFD AAVYSMTPER QEDMAPEILI TYGGHIVSKQ LKKYLRNHPP 

       310        320        330        340        350        360 
REHWHVAADG KIADLYGCLT TVIEMDPFEF LEKIAFLLDN KPTHYPLMWE NYCKTIPMPD 

       370        380        390        400        410        420 
LAYSEISVIG KLIRALPEPC ALHLANSSTV RYAQLFTVPP QVEICCNRGV NGIEGSLSTA 

       430        440        450        460        470        480 
IGYAAASSKL NFIIIGDLSF FYDMNALWNQ NYGANIRILL LNNEGGEIFH TLPGMDKSSR 

       490        500        510        520        530        540 
SREFITAEHY TTAKGWAEER GFIYMKVTGE EELEEAMQPF TSPETRMQPM LLEVFTDKEK 

       550 
DTTLLREYYH GLKNKNE 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000139 Genomic DNA. Translation: ABR40077.1.
RefSeqYP_001299699.1. NC_009614.1.

3D structure databases

ProteinModelPortalA6L313.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING435590.BVU_2418.

Protocols and materials databases

DNASU5303382.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABR40077; ABR40077; BVU_2418.
GeneID5303382.
KEGGbvu:BVU_2418.
PATRIC21069786. VBIBacVul85104_2500.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1165.
HOGENOMHOG000218359.
KOK02551.
OMAHINLAYE.
OrthoDBEOG6NWBQW.
ProtClustDBCLSK2757504.

Enzyme and pathway databases

BioCycBVUL435590:GH96-2413-MONOMER.
UniPathwayUPA00079; UER00164.

Family and domain databases

HAMAPMF_01659. MenD.
InterProIPR004433. MenaQ_synth_MenD.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view]
PfamPF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFPIRSF004983. MenD. 1 hit.
TIGRFAMsTIGR00173. menD. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMEND_BACV8
AccessionPrimary (citable) accession number: A6L313
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 24, 2007
Last modified: February 19, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways