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Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / JCM 5826 / NBRC 14291 / NCTC 11154)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi158Zinc 1UniRule annotation1
Metal bindingi161Zinc 1UniRule annotation1
Metal bindingi175Zinc 2UniRule annotation1
Metal bindingi178Zinc 2UniRule annotation1
Metal bindingi201Zinc 2UniRule annotation1
Metal bindingi204Zinc 2UniRule annotation1
Metal bindingi215Zinc 1UniRule annotation1
Metal bindingi218Zinc 1UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri145 – 227CR-typePROSITE-ProRule annotationAdd BLAST83

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperoneUniRule annotationSAAS annotation
Biological processDNA replicationUniRule annotation, Stress responseUniRule annotation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJUniRule annotation
Gene namesi
Name:dnaJUniRule annotation
Ordered Locus Names:BVU_2329Imported
OrganismiBacteroides vulgatus (strain ATCC 8482 / DSM 1447 / JCM 5826 / NBRC 14291 / NCTC 11154)Imported
Taxonomic identifieri435590 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
Proteomesi
  • UP000002861 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi435590.BVU_2329.

Structurei

3D structure databases

ProteinModelPortaliA6L2S4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 70JInterPro annotationAdd BLAST66
Domaini145 – 227CR-typeInterPro annotationAdd BLAST83
Repeati158 – 165CXXCXGXG motifUniRule annotation8
Repeati175 – 182CXXCXGXG motifUniRule annotation8
Repeati201 – 208CXXCXGXG motifUniRule annotation8
Repeati215 – 222CXXCXGXG motifUniRule annotation8

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri145 – 227CR-typePROSITE-ProRule annotationAdd BLAST83

Keywords - Domaini

RepeatUniRule annotationSAAS annotation, Zinc-fingerUniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105BZ5. Bacteria.
COG0484. LUCA.
HOGENOMiHOG000226717.
KOiK03686.
OMAiDMGGFAD.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
cd10719. DnaJ_zf. 1 hit.
Gene3Di1.10.287.110. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiView protein in InterPro
IPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
PfamiView protein in Pfam
PF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
PRINTSiPR00625. JDOMAIN.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.

Sequencei

Sequence statusi: Complete.

A6L2S4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKRDYYEVL EVDKTATLDV IKKAYRKKAI QYHPDKNPGD KEAEEKFKEA
60 70 80 90 100
AEAYDVLSNP DKRARYDQFG HAGMSGAAGG GFEGFGQGMS MDDIFSMFGD
110 120 130 140 150
IFGGHGGGFG GFGGFGGGGR SAQRKFRGSD LRVKVKLNLK EISTGVEKKF
160 170 180 190 200
KLKKYVTCDH CHGSGAEGEG GTETCPTCHG TGSITRTQQS IFGMVQSQSV
210 220 230 240 250
CPQCNGEGKI IKNKCKACAG EGIVYGEEVV EVKIPAGVAE GMQLSVNGKG
260 270 280 290 300
NAGKHNGVPG DLLVVIEEES HPDLIRDEND LIYNLLLSVP TAALGGTVEI
310 320 330 340 350
PTIDSKVKVK IEPGTQPGKV LRLRGKGLPN VNSYGYSNGT GDLLVNVSVY
360 370 380 390
IPETLNKDEK QALEKMQESD NFKPNTSIKE KIFKKFKNFF D
Length:391
Mass (Da):42,199
Last modified:July 24, 2007 - v1
Checksum:i67DD565177CB437D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000139 Genomic DNA. Translation: ABR39988.1.
RefSeqiWP_005846209.1. NC_009614.1.

Genome annotation databases

EnsemblBacteriaiABR39988; ABR39988; BVU_2329.
GeneIDi5303293.
KEGGibvu:BVU_2329.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000139 Genomic DNA. Translation: ABR39988.1.
RefSeqiWP_005846209.1. NC_009614.1.

3D structure databases

ProteinModelPortaliA6L2S4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi435590.BVU_2329.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR39988; ABR39988; BVU_2329.
GeneIDi5303293.
KEGGibvu:BVU_2329.

Phylogenomic databases

eggNOGiENOG4105BZ5. Bacteria.
COG0484. LUCA.
HOGENOMiHOG000226717.
KOiK03686.
OMAiDMGGFAD.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
cd10719. DnaJ_zf. 1 hit.
Gene3Di1.10.287.110. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiView protein in InterPro
IPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
PfamiView protein in Pfam
PF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
PF00684. DnaJ_CXXCXGXG. 1 hit.
PRINTSiPR00625. JDOMAIN.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
TIGRFAMsiTIGR02349. DnaJ_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS51188. ZF_CR. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiA6L2S4_BACV8
AccessioniPrimary (citable) accession number: A6L2S4
Entry historyiIntegrated into UniProtKB/TrEMBL: July 24, 2007
Last sequence update: July 24, 2007
Last modified: June 7, 2017
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.