A6L2M8 (GLAB2_BACV8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,3-galactosidase B
EC=3.2.1.n1
EC=3.2.1.n2

Alternative name(s):
Exo-alpha-galactosidase B2
EC=3.2.1.22

Gene names

Name:	glaB2
Ordered Locus Names:	BVU_2283

Organism

Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / NCTC 11154) [Complete proteome] [HAMAP]

Taxonomic identifier

435590 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	605 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Alpha-galactosidase. Removes both branched alpha-1,3-linked galactose residues of blood group B antigens and linear alpha-1,3-linked galactose structures By similarity.
Catalytic activity	Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-galactosidic residues, producing free D-galactose. Hydrolysis of terminal, non-reducing linear (1->3)-alpha-D-galactosidic residues, producing free D-galactose. Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.
Sequence similarities	Belongs to the glycosyl hydrolase 110 family. B subfamily. Contains 3 PbH1 repeats.

Ontologies

Keywords
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	raffinose alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 605	586	Alpha-1,3-galactosidase B		PRO_0000348480
Regions
	Repeat	428 – 450	23	PbH1 1
	Repeat	451 – 473	23	PbH1 2
	Repeat	484 – 538	55	PbH1 3

Sequences

	Sequence		Length	Mass (Da)
	A6L2M8 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: E7385BBB5AB7ADFF	FASTA	605	68,957
	10 20 30 40 50 60 MKRIIFNFCF VWLAVSAFAG SKVVEMRNYG LVPDTHENLS PKLQKALQDI KSQVALGDKV 70 80 90 100 110 120 TLLFESGRYD FHPEGAAVRE YYISNHDQDN PKTVGFPLED WKGLTVDGQG ADFIFHGRML 130 140 150 160 170 180 PLSLLRSENC TLRNFSIDFE TPHIAQVKIL ESGEEGITFE PAAWVKCRIN EKGFFEAYGE 190 200 210 220 230 240 GWSSAPQGGI AFEEKTKRLV YRTSDLWCPM EGVKEVSPRV YHAPQWKDAR LKPGTVVALR 250 260 270 280 290 300 TYYRPAPGIF LSNDKDTRLQ NVKVHYAEGM GLLAQLCENI TLDEFSVCLR GDKDPRYFTT 310 320 330 340 350 360 QADATHFSSC RGKIDSRNGL YEGMMDDAIN VHGTYLKIKQ RLDDHTVIAR YMHPQAYGFE 370 380 390 400 410 420 WGVNGDEVQF VRSATMELTG GKNRVKEILP NDKDTVKGAK EYRITFAEPL DAEITDKEGF 430 440 450 460 470 480 GIENLSWCPE VYFADNVIRN NRARGTLFST PLKTVVERNL FDHTSGTAIL LCGDCNGWFE 490 500 510 520 530 540 TGACRNVLIR NNRFINALTN MFQFTEAVIS IYPEIPDLEH QKKYFHGGKG EKGVVIEDNY 550 560 570 580 590 600 FETFDRPVLF AKSIDGLIFK NNVIRQNTDY PAFHHNKSRF RLLHTRNVKI EKNNFEDGDE SIARE

References

[1]

"Evolution of symbiotic bacteria in the distal human intestine." Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C., Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H., Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K., Knight R.D., Gordon J.I. PLoS Biol. 5:1574-1586(2007) [PubMed: 17579514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8482 / DSM 1447 / NCTC 11154.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000139 Genomic DNA. Translation: ABR39942.1.
RefSeq	YP_001299564.1. NC_009614.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6L2M8.
Protein family/group databases
CAZy	GH110. Glycoside Hydrolase Family 110.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5303247.
GenomeReviews	Gene locus BVU_2283 in contig CP000139_GR.
KEGG	bvu:BVU_2283.
NMPDR	fig|435590.6.peg.2141.
PATRIC	21069496. VBIBacVul85104_2355.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG77539.
HOGENOM	HBG345842.
OMA	ATHFANC.
ProtClustDB	CLSK823424.
Family and domain databases
InterPro	IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 3 hits.
SMART	SM00710. PbH1. 4 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLAB2_BACV8

Accession

Primary (citable) accession number: A6L2M8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	July 24, 2007
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program