Reviewed, UniProtKB/Swiss-Prot A6L1X1 (PURA_BACV8)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase
    EC=6.3.4.4
Alternative name(s):
    IMP--aspartate ligase
    AdSS
    AMPSase

Gene names

Name:	purA
Ordered Locus Names:	BVU_2018

Organism

Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / NCTC 11154) [Complete proteome] [HAMAP]

Taxonomic identifier

435590 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	423 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis. HAMAP MF_00011
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. HAMAP MF_00011
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. HAMAP MF_00011
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP adenylosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 423	423	Adenylosuccinate synthetase HAMAP MF_00011		PRO_1000000780
Regions
	Nucleotide binding	12 – 18	7	GTP Potential
Sites
	Active site	140	1	By similarity
	Active site	147	1	By similarity
	Metal binding	13	1	Magnesium By similarity
	Metal binding	40	1	Magnesium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6L1X1-1 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: F7B0A7457685CA95	FASTA	423	46,820
	10 20 30 40 50 60 MKVDVLLGLQ WGDEGKGKVV DVLTPRYDVV ARFQGGPNAG HTLEFEGQKY VLRSIPSGIF 70 80 90 100 110 120 QGNKVNIIGN GVVLDPALFK AEAEALEASG HPLKERLHIS KKAHLILPTH RILDAAYEAA 130 140 150 160 170 180 KGDAKVGTTG KGIGPTYTDK VSRNGVRVGD ILHNFEEVYG KAKARHEQIL KSLNYEYDIT 190 200 210 220 230 240 ELEKQWLEGI EYLKQFHLVD SEHEINNLLK SGKSVLCEGA QGTMLDVDFG SYPFVTSSNT 250 260 270 280 290 300 ICAGACTGLG IGPNKIGNVY GIMKAYCTRV GAGPFPTELF DETGKKIRDL GHEYGAVTGR 310 320 330 340 350 360 ERRCGWIDLV ALKYSIMVNG VTQLIMMKSD VLDDFETIKA CVAYKVNGEE IDYFPYDISE 370 380 390 400 410 420 GLEPVYAELP GWKTDMTKMT SEDEFPEEFN AYVTFLEEQL ETPIKIVSVG PDRGQTIERY TEE

References

[1]

"Evolution of symbiotic bacteria in the distal human intestine." Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C., Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H., Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K., Knight R.D., Gordon J.I. PLoS Biol. 5:1574-1586(2007) [PubMed: 17579514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000139 Genomic DNA. Translation: ABR39685.1.
RefSeq	YP_001299307.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6L1X1.
Genome annotation databases
GeneID	5302984.
GenomeReviews	Gene locus BVU_2018 in contig CP000139_GR.
KEGG	bvu:BVU_2018.
NMPDR	fig|435590.6.peg.1896.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	IPVCVAY.
Family and domain databases
HAMAP	MF_00011. [Tree]
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
ProDom	PD001188. Asucc_synthtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00184. purA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA_BACV8

Accession

Primary (citable) accession number: A6L1X1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 24, 2007
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)