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A6L1U8

- LIPA_BACV8

UniProt

A6L1U8 - LIPA_BACV8

Protein

Lipoyl synthase

Gene

lipA

Organism
Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / NCTC 11154)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (24 Jul 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi36 – 361Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi41 – 411Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi47 – 471Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi62 – 621Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi66 – 661Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi69 – 691Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciBVUL435590:GH96-1987-MONOMER.
    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthaseUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lip-synUniRule annotation
    Short name:
    LSUniRule annotation
    Lipoate synthaseUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Sulfur insertion protein LipAUniRule annotation
    Gene namesi
    Name:lipAUniRule annotation
    Ordered Locus Names:BVU_1990
    OrganismiBacteroides vulgatus (strain ATCC 8482 / DSM 1447 / NCTC 11154)
    Taxonomic identifieri435590 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
    ProteomesiUP000002861: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 284284Lipoyl synthasePRO_0000325235Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi435590.BVU_1990.

    Structurei

    3D structure databases

    ProteinModelPortaliA6L1U8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0320.
    HOGENOMiHOG000235997.
    KOiK03644.
    OMAiHPHIPTK.
    OrthoDBiEOG6038ZS.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A6L1U8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNHVKKPDWL KINIGANARY TETKHIVDSH KLHTICSSGR CPNMGECWGK    50
    GTATFMIGGE ICTRSCKFCN TQTGRPLPLD PEEPTHVAES IQLMKLSHAV 100
    ITSVDRDDLD DLGAAHWAKT ISEIKRLNPE TTTEVLIPDF QGKSELIQLV 150
    IDAKPDIISH NMETVRRISP LVRSAANYAT SLKVIKQIAN NGITAKSGIM 200
    VGLGERPEEV EEVMDDLLAQ GCKILTIGQY LQPTHRHYPV AEYITPDQFK 250
    QYRTIGLKKG FREVESAPLV RSSYHAEKHI KFKG 284
    Length:284
    Mass (Da):31,725
    Last modified:July 24, 2007 - v1
    Checksum:i77A9B74590DFD3A6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000139 Genomic DNA. Translation: ABR39662.1.
    RefSeqiYP_001299284.1. NC_009614.1.

    Genome annotation databases

    EnsemblBacteriaiABR39662; ABR39662; BVU_1990.
    GeneIDi5302956.
    KEGGibvu:BVU_1990.
    PATRICi21068890. VBIBacVul85104_2061.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000139 Genomic DNA. Translation: ABR39662.1 .
    RefSeqi YP_001299284.1. NC_009614.1.

    3D structure databases

    ProteinModelPortali A6L1U8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 435590.BVU_1990.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABR39662 ; ABR39662 ; BVU_1990 .
    GeneIDi 5302956.
    KEGGi bvu:BVU_1990.
    PATRICi 21068890. VBIBacVul85104_2061.

    Phylogenomic databases

    eggNOGi COG0320.
    HOGENOMi HOG000235997.
    KOi K03644.
    OMAi HPHIPTK.
    OrthoDBi EOG6038ZS.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .
    BioCyci BVUL435590:GH96-1987-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 8482 / DSM 1447 / NCTC 11154.

    Entry informationi

    Entry nameiLIPA_BACV8
    AccessioniPrimary (citable) accession number: A6L1U8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: July 24, 2007
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3