Reviewed, UniProtKB/Swiss-Prot A6L1L8 (SYA_BACV8)

Names and origin

Protein names

Recommended name:
    Alanyl-tRNA synthetase
    EC=6.1.1.7
Alternative name(s):
    Alanine--tRNA ligase
      Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	BVU_1908

Organism

Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / NCTC 11154) [Complete proteome] [HAMAP]

Taxonomic identifier

435590 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	871 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036
Subcellular location	Cytoplasm By similarity HAMAP MF_00036.
Domain	The C-terminal C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP alanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 871	871	Alanyl-tRNA synthetase HAMAP MF_00036		PRO_0000347502

Sequences

	Sequence		Length	Mass (Da)
	A6L1L8-1 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: E4BE5DE0039667B8	FASTA	871	97,262
	10 20 30 40 50 60 MTAKEIRDSF KSFFESKGHQ IVPSAPMVIK DDPTLMFTNA GMNQFKDIIL GNHPAKYKRV 70 80 90 100 110 120 ADSQKCLRVS GKHNDLEEVG HDTYHHTMFE MLGNWSFGDY FKKEAIGWAW EYLVDVLKID 130 140 150 160 170 180 PKDLYATVFE GSPEEGLERD NEAASYWEQF LPKDHILNGN KHDNFWEMGD TGPCGPCSEI 190 200 210 220 230 240 HIDSRSEEEK AQIPGNQLVN KDHPQVIEIW NLVFMQFNRK ADGSLEGLPA KVIDTGMGFE 250 260 270 280 290 300 RLVRTLQGKT SNYDTDVFQP ILKAIAEMAG TTYGQDNQKD IAMRVIADHI RTIAFSITDG 310 320 330 340 350 360 QLPSNAKAGY VIRRILRRAV RYGYTFLGQK QAFMYKLLPV LIENMGEAYP ELNAQKTLIE 370 380 390 400 410 420 KVIKEEEESF LRTLETGIRL LDKTMNDAKA AGKKEISGVD AFTLYDTFGF PLDLTELILR 430 440 450 460 470 480 ENGMTVNEEE FNAEMQKQKE RARNAAAVET GDWITIKEGD THFVGYDFTE YETSILRYRQ 490 500 510 520 530 540 IKQKNQTLYQ IVLSDTPFYA ESGGQVGDTG VIVSEFETIE IIDTKKENNL PIHITKKLPE 550 560 570 580 590 600 HLDVPMMACV DTEKRAACAA NHSCTHLLDE ALRQVLGTHV EQKGSLVTPE SLRFDFSHFQ 610 620 630 640 650 660 KVTDEQLREV EHLVNAKIRE NIPLTEYRNL PIEKAKELGA IALFGEKYGD EVRVVQFGNS 670 680 690 700 710 720 IEFCGGTHVP ATGKIGMVRI ISESSVAAGV RRIEAITGAK VEELMDTVQD TLNDLKALFN 730 740 750 760 770 780 NAPDLKVAIR KYIDENAGLK KQVEEFMKEK GAALKARLVE NAKEINGVKV VKAIIPMSAD 790 800 810 820 830 840 VVKDIAFQLK GEIPANLFVV IGSVDNNKPM LTVMISEDLV KAGQNAGKLV REAAKLIQGG 850 860 870 GGGQPHFATA GGKNPDGLNA AVDKVIELAA L

References

[1]

"Evolution of symbiotic bacteria in the distal human intestine." Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C., Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H., Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K., Knight R.D., Gordon J.I. PLoS Biol. 5:1574-1586(2007) [PubMed: 17579514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000139 Genomic DNA. Translation: ABR39582.1.
RefSeq	YP_001299204.1.
3D structure databases
SMR	A6L1L8. Positions 1-468, 469-695.
ModBase	Search...
Protein-protein interaction databases
STRING	A6L1L8.
Genome annotation databases
GeneID	5302874.
GenomeReviews	Gene locus BVU_1908 in contig CP000139_GR.
KEGG	bvu:BVU_1908.
NMPDR	fig|435590.6.peg.1794.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0013.
HOGENOM	HBG354397.
OMA	TLMFTNS.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00344. alaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_BACV8

Accession

Primary (citable) accession number: A6L1L8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	July 24, 2007
Last modified:	February 9, 2010
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)