A6L0E8 (SYE_BACV8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	BVU_1475

Organism

Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / NCTC 11154) [Complete proteome] [HAMAP]

Taxonomic identifier

435590 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 505	505	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_1000001872
Regions
	Motif	12 – 22	11	"HIGH" region HAMAP MF_00022_B
	Motif	260 – 264	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	263	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6L0E8 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: B2E721D705D60FD3	FASTA	505	57,874
	10 20 30 40 50 60 MSERKVRVRF APSPTGALHI GGVRTALYNY LFARQHGGDL IFRIEDTDSN RFVPGAEEYI 70 80 90 100 110 120 IESFKWLGIN FDEGVSFGGN YGPYRQSERR DIYKKYVQVL LDSGKAYIAF DTPAELDAKR 130 140 150 160 170 180 QEISNFQYDA STRMSMRNSL TLPKEEVDAL IADGKQYVVR FKIEPNEDVH VHDIIRGEVV 190 200 210 220 230 240 INSSILDDKV LYKSADELPT YHLANIVDDH LMEVSHVIRG EEWLPSAPLH VLLYRAFGWA 250 260 270 280 290 300 DTMPEFAHLP LLLKPDGNGK LSKRDGDRLG FPVFPLEWHD PKTGEVSSGY RESGYLPEAV 310 320 330 340 350 360 INFLALLGWN PGNDQELMSL DELVKLFDLH RCSKAGAKFD FEKGKWFNHE YILKKSNEEV 370 380 390 400 410 420 AGLFMPILKE HGIEAPMDKV VTVVGLMKDR VSFIKDLWET CKFFFVAPTE YDEKTRKKRW 430 440 450 460 470 480 KEDSPERMLE LADVLEALDD FSLENQEAVV MKWIEDKGYH LGNIMNAFRL TLVGEGKGPH 490 500 MFDISAVLGK EETLRRIRRA VEVLK

References

[1]

"Evolution of symbiotic bacteria in the distal human intestine." Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C., Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H., Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K., Knight R.D., Gordon J.I. PLoS Biol. 5:1574-1586(2007) [PubMed: 17579514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8482 / DSM 1447 / NCTC 11154.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000139 Genomic DNA. Translation: ABR39162.1.
RefSeq	YP_001298784.1. NC_009614.1.
3D structure databases
ProteinModelPortal	A6L0E8.
ModBase	Search...
Protein-protein interaction databases
STRING	A6L0E8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5302441.
GenomeReviews	Gene locus BVU_1475 in contig CP000139_GR.
KEGG	bvu:BVU_1475.
NMPDR	fig|435590.6.peg.1393.
PATRIC	21067812. VBIBacVul85104_1537.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	IEWFNLD.
ProtClustDB	PRK01406.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_BACV8

Accession

Primary (citable) accession number: A6L0E8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 24, 2007
Last modified:	January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program