A6L083 (DUT_BACV8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	BVU_1409

Organism

Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / NCTC 11154) [Complete proteome] [HAMAP]

Taxonomic identifier

435590 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	144 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP MF_00116
Cofactor	Magnesium By similarity. HAMAP MF_00116
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 144	144	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP MF_00116		PRO_1000015444
Regions
	Region	63 – 65	3	Substrate binding By similarity
	Region	80 – 82	3	Substrate binding By similarity
Sites
	Binding site	76	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6L083 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: 8806EF0FF90FEB5A	FASTA	144	15,779
	10 20 30 40 50 60 MKIQVINKSK HALPEYATGQ SAGMDIRANL DEPIVLKPLQ RCLVPTGLYI ALPEGFEAQI 70 80 90 100 110 120 RPRSGLAIKK GIGVLNSPGT IDADYRGEIC IILVNLSSED FMIEDGERIA QMVVARHEHA 130 140 EWQEVEVLDE TERGAGGFGH TGKK

References

[1]

"Evolution of symbiotic bacteria in the distal human intestine." Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C., Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H., Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K., Knight R.D., Gordon J.I. PLoS Biol. 5:1574-1586(2007) [PubMed: 17579514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8482 / DSM 1447 / NCTC 11154.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000139 Genomic DNA. Translation: ABR39097.1.
RefSeq	YP_001298719.1. NC_009614.1.
3D structure databases
ProteinModelPortal	A6L083.
ModBase	Search...
Protein-protein interaction databases
STRING	A6L083.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5302375.
GenomeReviews	Gene locus BVU_1409 in contig CP000139_GR.
KEGG	bvu:BVU_1409.
NMPDR	fig|435590.6.peg.1331.
PATRIC	21067676. VBIBacVul85104_1470.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0756.
HOGENOM	HBG436079.
OMA	AQMVFAP.
ProtClustDB	PRK00601.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact. [Tree]
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
KO	K01520.
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. Dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_BACV8

Accession

Primary (citable) accession number: A6L083

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 24, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program