Reviewed, UniProtKB/Swiss-Prot A6L075 (MURE_BACV8)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
    EC=6.3.2.13
Alternative name(s):
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    Meso-diaminopimelate-adding enzyme
    Meso-A2pm-adding enzyme
    UDP-N-acetylmuramyl-tripeptide synthetase
    UDP-MurNAc-tripeptide synthetase

Gene names

Name:	murE
Ordered Locus Names:	BVU_1401

Organism

Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / NCTC 11154) [Complete proteome] [HAMAP]

Taxonomic identifier

435590 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity.
Sequence similarities	Belongs to the murCDEF family. MurE subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208		PRO_1000012340
Regions
	Nucleotide binding	111 – 117	7	ATP Potential
	Region	153 – 154	2	UDP-MurNAc-L-Ala-D-Glu binding By similarity
	Region	402 – 405	4	Meso-diaminopimelate binding By similarity
	Motif	402 – 405	4	Meso-diaminopimelate recognition motif HAMAP MF_00208
Sites
	Binding site	30	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	180	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	186	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	188	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	378	1	Meso-diaminopimelate By similarity
	Binding site	455	1	Meso-diaminopimelate; via carbonyl oxygen By similarity
	Binding site	459	1	Meso-diaminopimelate By similarity
Amino acid modifications
	Modified residue	220	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6L075-1 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: CAEC00C575D9AA16	FASTA	484	53,283
	10 20 30 40 50 60 MKLEKIIKGI TVNEIIGDAS QEISGINMDS RLIEPGHIFV AVKGTQTDGH TYIQKAIEKG 70 80 90 100 110 120 ARTVVCENLP ETLIENVTYI KVNDTEDVVG KLATTFYGDP TSKLELVGVT GTNGKTTIAT 130 140 150 160 170 180 LLYNMFRKFG YKTGLISTVC NYIDEEAIPT DHTTPDPITL NKLLGRMADE GCKYAFMEVS 190 200 210 220 230 240 SHSVAQKRIG GLKFAGGIFT NLTRDHLDYH KTVENYLKAK KAFFDGLPKT AFALTNLDDK 250 260 270 280 290 300 NGLVMTQNTK AKVHTYSLRS LSDFKGKVLE DGFEGMLLDI NNVEVNVQFI GRFNASNLLA 310 320 330 340 350 360 VYGAACLLGK KTEEVLLALS TLRPVAGRFD SLRSPKGYTA IVDYAHTPDA LENVLNAIHE 370 380 390 400 410 420 VLNGKGHVIT VVGAGGNRDK GKRPLMAQEA VKQSDKVIIT SDNPRFEEPQ EIINDMLAGL 430 440 450 460 470 480 TKEDMRKVIS IADRKEAIRT ACMLAQAKDV ILVAGKGHEN YQEIKGIKHH FDDKEVLRDI FANE

References

[1]

"Evolution of symbiotic bacteria in the distal human intestine." Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C., Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H., Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K., Knight R.D., Gordon J.I. PLoS Biol. 5:1574-1586(2007) [PubMed: 17579514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000139 Genomic DNA. Translation: ABR39089.1.
RefSeq	YP_001298711.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6L075.
Genome annotation databases
GeneID	5302367.
GenomeReviews	Gene locus BVU_1401 in contig CP000139_GR.
KEGG	bvu:BVU_1401.
NMPDR	fig|435590.6.peg.1323.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FPVIVDY.
Family and domain databases
HAMAP	MF_00208. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase. [Graphical view]
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01085. murE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURE_BACV8

Accession

Primary (citable) accession number: A6L075

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 24, 2007
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)