A6L074 (MRAY_BACV8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phospho-N-acetylmuramoyl-pentapeptide-transferase
EC=2.7.8.13

Alternative name(s):
UDP-MurNAc-pentapeptide phosphotransferase

Gene names

Name:	mraY
Ordered Locus Names:	BVU_1400

Organism

Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / NCTC 11154) [Complete proteome] [HAMAP]

Taxonomic identifier

435590 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity. HAMAP MF_00038
Catalytic activity	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_00038.
Sequence similarities	Belongs to the glycosyltransferase 4 family. MraY subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine:undecaprenyl-phosphate transferase activity Inferred from electronic annotation. Source: EC phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038		PRO_1000074532
Regions
	Transmembrane	28 – 48	21	Helical; Potential
	Transmembrane	71 – 91	21	Helical; Potential
	Transmembrane	95 – 115	21	Helical; Potential
	Transmembrane	136 – 156	21	Helical; Potential
	Transmembrane	208 – 228	21	Helical; Potential
	Transmembrane	239 – 259	21	Helical; Potential
	Transmembrane	279 – 299	21	Helical; Potential
	Transmembrane	313 – 333	21	Helical; Potential
	Transmembrane	399 – 419	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	A6L074 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: 25C485AD610DBB39	FASTA	422	47,026
	10 20 30 40 50 60 MLYYLFQYLE KFDFPGAGMF GYVSFRSLMA IILSLLISAI FGEYFINLLK RKQITETQRD 70 80 90 100 110 120 ASIDPFNVKK VGVPTMGGII IIVAILIPCL LLGKLHNIYM ILMLITTIWL GTLGFLDDYI 130 140 150 160 170 180 KVFRKDKEGL HGKFKIIGQV GLGLIVGLTL YLSPSVVIRE NVEIQRGGEI VEVVHKEQDQ 190 200 210 220 230 240 KSTKTTIPFF KNNNLDYADF VGFLGNNAQA VGWIIFVLVT IFVVTAVSNG ANLNDGMDGM 250 260 270 280 290 300 AAGNSAIIGL TLGILAYVSS HIEYAGYLNI MYIPGSEELV IFICSFIGAL IGFLWYNAFP 310 320 330 340 350 360 AQVFMGDTGS LTIGGIIAVF AIIIHKELLI PILCGIFLVE NLSVILQVRY FKSGKKKGHL 370 380 390 400 410 420 QRVFKRAPIH DHFRTTLAQL DPNCSYLFMK PNSVFHESKI TVRFWIVTIV LAAITIITLK IR

References

[1]

"Evolution of symbiotic bacteria in the distal human intestine." Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C., Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H., Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K., Knight R.D., Gordon J.I. PLoS Biol. 5:1574-1586(2007) [PubMed: 17579514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8482 / DSM 1447 / NCTC 11154.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000139 Genomic DNA. Translation: ABR39088.1.
RefSeq	YP_001298710.1. NC_009614.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6L074.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5302366.
GenomeReviews	Gene locus BVU_1400 in contig CP000139_GR.
KEGG	bvu:BVU_1400.
NMPDR	fig|435590.6.peg.1322.
PATRIC	21067658. VBIBacVul85104_1461.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0472.
HOGENOM	HBG708263.
OMA	RFWIISM.
ProtClustDB	PRK00108.
Family and domain databases
HAMAP	MF_00038. MraY. [Tree]
InterPro	IPR000715. Glycosyl_transferase_4. IPR003524. PNAcMuramoyl-5peptid_Trfase. IPR018480. PNAcMuramoyl-5peptid_Trfase_CS. [Graphical view]
KO	K01000.
PANTHER	PTHR22926. Glyco_trans_4. 1 hit. PTHR22926:SF3. PNAcPpept_trans. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00445. MraY. 1 hit.
PROSITE	PS01347. MRAY_1. 1 hit. PS01348. MRAY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MRAY_BACV8

Accession

Primary (citable) accession number: A6L074

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 24, 2007
Last modified:	December 14, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program