Reviewed, UniProtKB/Swiss-Prot A6KZL8 (SYY_BACV8)

Names and origin

Protein names

Recommended name:
    Tyrosyl-tRNA synthetase
    EC=6.1.1.1
Alternative name(s):
    Tyrosine--tRNA ligase
      Short name=TyrRS

Gene names

Name:	tyrS
Ordered Locus Names:	BVU_1191

Organism

Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / NCTC 11154) [Complete proteome] [HAMAP]

Taxonomic identifier

435590 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr) By similarity.
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr). HAMAP MF_02006
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 1 subfamily. Contains 1 S4 RNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP RNA binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	Tyrosyl-tRNA synthetase HAMAP MF_02006		PRO_1000088578
Regions
	Domain	362 – 429	68	S4 RNA-binding
	Motif	37 – 46	10	"HIGH" region HAMAP MF_02006
	Motif	232 – 236	5	"KMSKS" region HAMAP MF_02006
Sites
	Binding site	32	1	Tyrosine By similarity
	Binding site	172	1	Tyrosine By similarity
	Binding site	176	1	Tyrosine By similarity
	Binding site	235	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6KZL8-1 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: 6CE81313F74A2D79	FASTA	430	48,041
	10 20 30 40 50 60 MNFVEELTWR GMVHTMMPGT EELLAKEQVT AYLGIDPTAD SLHIGHLCGV MMLRHFQRCG 70 80 90 100 110 120 HKPLALVGGA TGMIGDPSGK SQERNLLTEE TLRHNVACIK KQLAKFLDFE SDAPNKAELV 130 140 150 160 170 180 NNYDWMKDFT FLDFAREVGK HITVNYMMAK DSVQKRLNGE ARDGLSFTEF TYQLLQGYDF 190 200 210 220 230 240 LHLYETKGCK LQMGGSDQWG NITTGAELIR RTNGGEVFAL TCPLITKADG GKFGKTESGN 250 260 270 280 290 300 IWLDPRYTSP YKFYQFWLNV SDEDAARYIK IFTSLSQEEV EALTAEHAEA PHLRVLQKRL 310 320 330 340 350 360 AKEVTVMVHS EEDYNAAVEA SGILFGNATS EALKKLDEDT LLAVFEGVPQ FEVSRDALAE 370 380 390 400 410 420 GVKAVDLFVD NAAVFASKGE MRKLVQGGGV SLNKEKLSAF DQVITTADLL DEKYLLVQRG 430 KKNYYLVIAK

References

[1]

"Evolution of symbiotic bacteria in the distal human intestine." Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C., Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H., Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K., Knight R.D., Gordon J.I. PLoS Biol. 5:1574-1586(2007) [PubMed: 17579514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000139 Genomic DNA. Translation: ABR38882.1.
RefSeq	YP_001298504.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6KZL8.
Genome annotation databases
GeneID	5302157.
GenomeReviews	Gene locus BVU_1191 in contig CP000139_GR.
KEGG	bvu:BVU_1191.
NMPDR	fig|435590.6.peg.1125.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	TFYIGFD.
Family and domain databases
HAMAP	MF_02006. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ib. IPR002942. S4_RNA_bd. IPR002307. Tyr-tRNA-synth_Ib_bac/mito. [Graphical view]
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50889. S4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYY_BACV8

Accession

Primary (citable) accession number: A6KZL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	July 24, 2007
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)