Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / NCTC 11154)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei721 – 7211ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBVUL435590:GH96-1146-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:BVU_1149
OrganismiBacteroides vulgatus (strain ATCC 8482 / DSM 1447 / NCTC 11154)
Taxonomic identifieri435590 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
ProteomesiUP000002861 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 944944Leucine--tRNA ligasePRO_1000009294Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi435590.BVU_1149.

Structurei

3D structure databases

ProteinModelPortaliA6KZH6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5112"HIGH" regionAdd
BLAST
Motifi718 – 7225"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A6KZH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYNFREIEK KWQQRWAENH TYQVTEDESK KKFYVLNMFP YPSGAGLHVG
60 70 80 90 100
HPLGYIASDI YARYKRLQGF NVLNPMGYDA YGLPAEQYAI QTGQHPAITT
110 120 130 140 150
VNNINRYREQ LDKIGFSFDW NREVRTCEPG YYHWTQWAFQ QMFNSYYCND
160 170 180 190 200
TQQARPISEL TEAFARYGNE GLNAACSEEL SFTAEEWNAK SEKEQQEILM
210 220 230 240 250
NYRIAYLGET MVNWCPQLGT VLANDEVVDG VSERGGFPVV QKKMRQWCLR
260 270 280 290 300
VSAYAQRLLD GLDTVDWTDS LKETQRNWIG RSEGTEVQFK VKDSDIEFTI
310 320 330 340 350
FTTRADTMFG VTFMVLAPES ELVPQLTTEA QKAEVEAYLD RTKKRTERER
360 370 380 390 400
IADRRVTGVF SGSYAINPFT GEAVPVWISD YVLAGYGTGA IMAVPAHDSR
410 420 430 440 450
DYAFAKHFNL PIVPLVEGCD VSEESFDAKE GIVCNSPRKD VTPYCDLSLN
460 470 480 490 500
GLTIKEAIAA TKEYVKAHNL GRVKVNYRLR DAIFSRQRYW GEPFPVYYKN
510 520 530 540 550
GMPYMIDSSK LPLELPEVAK FLPTETGEPP LGHATKWAWD VEKGEVVENN
560 570 580 590 600
LIDNVTIFPL ELNTMPGFAG SSAYYLRYMD PHNAQALVSE KADHYWQNVD
610 620 630 640 650
LYVGGTEHAT GHLIYSRFWN KFLHDLGVSI KEEPFQKLVN QGMIQGRSNF
660 670 680 690 700
VYRIKDTNTF VSLNLKDQYE TTPLHVDVNI VSNDILDVEA FKAWRPEYND
710 720 730 740 750
AEFILEDGKY ICGWAVEKMS KSMYNVVNPD MIVEKYGADT LRMYEMFLGP
760 770 780 790 800
VEQSKPWDTN GIDGVHRFLK KLWNLFYSRT DEFLPVEGEP TKEELKAIHK
810 820 830 840 850
LIKKVTGDIE TFSYNTSISA FMICVNELTS LKCRNKEVLS NLIILLAPFA
860 870 880 890 900
PHYAEELWEA LGNTTSVCDA QWPAFNEDYL KEDTVKYTIS FNGKARFTMD
910 920 930 940
FAADADNNTI QTTVMADEQA QKWIEGKTPK KVIIVPKKIV NIVL
Length:944
Mass (Da):108,274
Last modified:July 23, 2007 - v1
Checksum:i90C19E42471E1C73
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000139 Genomic DNA. Translation: ABR38840.1.
RefSeqiYP_001298462.1. NC_009614.1.

Genome annotation databases

EnsemblBacteriaiABR38840; ABR38840; BVU_1149.
KEGGibvu:BVU_1149.
PATRICi21067120. VBIBacVul85104_1195.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000139 Genomic DNA. Translation: ABR38840.1.
RefSeqiYP_001298462.1. NC_009614.1.

3D structure databases

ProteinModelPortaliA6KZH6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi435590.BVU_1149.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR38840; ABR38840; BVU_1149.
KEGGibvu:BVU_1149.
PATRICi21067120. VBIBacVul85104_1195.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200748.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciBVUL435590:GH96-1146-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 8482 / DSM 1447 / NCTC 11154.

Entry informationi

Entry nameiSYL_BACV8
AccessioniPrimary (citable) accession number: A6KZH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 14, 2008
Last sequence update: July 23, 2007
Last modified: March 31, 2015
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.