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A6KYR3 (SYM_BACV8) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase
EC=6.1.1.10
Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS
Gene names
Name:metG
Ordered Locus Names:BVU_0882
OrganismBacteroides vulgatus (strain ATCC 8482 / DSM 1447 / NCTC 11154) [Complete proteome] [HAMAP]
Taxonomic identifier435590 [NCBI]
Taxonomic lineageBacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length680 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098

Subunit structure

Homodimer By similarity. HAMAP MF_00098

Subcellular location

Cytoplasm By similarity HAMAP MF_00098.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Contains 1 tRNA-binding domain.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
RNA-binding
Zinc
tRNA-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processmethionyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

methionine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 680680Methionine--tRNA ligase HAMAP MF_00098
PRO_0000331782

Regions

Domain578 – 680103tRNA-binding
Motif15 – 2511"HIGH" region HAMAP MF_00098
Motif332 – 3365"KMSKS" region HAMAP MF_00098

Sites

Metal binding1471Zinc By similarity
Metal binding1501Zinc By similarity
Metal binding1601Zinc By similarity
Metal binding1631Zinc By similarity
Binding site3351ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A6KYR3 [UniParc].

Last modified July 24, 2007. Version 1.
Checksum: ACA1365D5EC79E3C

FASTA68077,704
        10         20         30         40         50         60 
MENKFKRTTV TSALPYANGP VHIGHLAGVY VPADIYVRYL RLKKEDVLFI GGSDEHGVPI 

        70         80         90        100        110        120 
TIRAKKEGIT PQDVVDRYHT LIKESFKEFG ISFDIYSRTT SKTHHELASE FFKTLYDKGE 

       130        140        150        160        170        180 
FIEKTSMQYY DEEAHQFLAD RYITGECPHC HAEGAYGDQC EKCGTSLSPT DLINPKSAIS 

       190        200        210        220        230        240 
GSKPVMRETK HWYLPLDKHE GWLREWILEN HKEWRPNVYG QCKSWLDMGL QPRAVSRDLD 

       250        260        270        280        290        300 
WGIPVPVEGA EGKVLYVWFD APIGYISNTK ELLPDSWETW WKDPETRLIH FIGKDNIVFH 

       310        320        330        340        350        360 
CIVFPAMLKA EGSYILPDNV PSNEFLNLEG DKISTSRNWA VWLHEYLQDF PGKQDVLRYV 

       370        380        390        400        410        420 
LTANAPETKD NDFTWKDFQA RNNNELVAVY GNFVNRALVL THKYFDGKVP VCGELTDYDK 

       430        440        450        460        470        480 
ETLKEFADVK AEVEKLLDVF KFRDAQKEAM NLARIGNKYL ADTEPWKLAK TDMDRVATIL 

       490        500        510        520        530        540 
NIALQLVANL AIAFEPFLPF SSEKLRKMLN METFEWDQLG RTDLLAEGHQ LNKAELLFEK 

       550        560        570        580        590        600 
IEDETIQAQV DKLLATKKAN EAANYKANPI KPVIAFEEFE KLDIRVGTVL ECEVVPKMKK 

       610        620        630        640        650        660 
LLKFKIADGL ENRTIVSGIA QHYKPEELVG KQVLFIANLA PRQFKNGLVS EGMILSAENF 

       670        680 
DGTLAVTSLL REVKPGSEVK

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000139 Genomic DNA. Translation: ABR38577.1.
RefSeqYP_001298199.1. NC_009614.1.

3D structure databases

ProteinModelPortalA6KYR3.
ModBaseSearch...

Protein-protein interaction databases

STRINGA6KYR3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5301849.
GenomeReviewsGene locus BVU_0882 in contig CP000139_GR.
KEGGbvu:BVU_0882.
NMPDRfig|435590.6.peg.840.
PATRIC21066538. VBIBacVul85104_0906.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0143.
HOGENOMHBG721667.
OMAEEKTRNV.
ProtClustDBPRK00133.

Family and domain databases

HAMAPMF_00098. Met_tRNA_synth_type1.
[Tree]
InterProIPR015413. aa-tRNA-synt_I.
IPR001412. aa-tRNA-synth_I_CS.
IPR004495. Met-tRNA-synth_Ia_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023458. Met-tRNA_synth_1.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KOK01874.
PfamPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. MetG. 1 hit.
TIGR00399. MetG_C_term. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYM_BACV8
AccessionPrimary (citable) accession number: A6KYR3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: July 24, 2007
Last modified: January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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