Reviewed, UniProtKB/Swiss-Prot A6KXU3 (DDL_BACV8)

Names and origin

Protein names

Recommended name:
    D-alanine--D-alanine ligase
    EC=6.3.2.4
Alternative name(s):
    D-alanylalanine synthetase
    D-Ala-D-Ala ligase

Gene names

Name:	ddl
Ordered Locus Names:	BVU_0547

Organism

Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / NCTC 11154) [Complete proteome] [HAMAP]

Taxonomic identifier

435590 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation By similarity.
Catalytic activity	ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro D-alanine-D-alanine ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	D-alanine--D-alanine ligase		PRO_1000030427
Regions
	Domain	121 – 321	201	ATP-grasp
	Nucleotide binding	149 – 204	56	ATP By similarity
Sites
	Metal binding	275	1	Magnesium or manganese 1 By similarity
	Metal binding	288	1	Magnesium or manganese 1 By similarity
	Metal binding	288	1	Magnesium or manganese 2 By similarity
	Metal binding	290	1	Magnesium or manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6KXU3-1 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: 36C9C8C02F858E54	FASTA	324	36,065
	10 20 30 40 50 60 MKRTIAIVAG GDSSELVVSL RSAQGLYSFI DKERYNLYIV EMEGHRWEVV LPDGSKTPID 70 80 90 100 110 120 RNDFSFMENG EKKQFDFAYI TIHGTPGENG ILQGYFDLLG IPYSSCNVLV SAMTFNKFTC 130 140 150 160 170 180 NQYLKGFGIR VAESLILRKG FEITDEEVIN KIGLPCFIKP NAGGSSFGVT KVKTKEDIQP 190 200 210 220 230 240 AIEKAFEESD EVMIEAFMKG TEITCGCYKT SDKEVVFPIT EVVSANEFFD YGAKYNGESQ 250 260 270 280 290 300 EITPARLPED TAERVRLLTS AIYDILGCSG LIRIDYIITE GEKVNLLEIN TTPGMTATSF 310 320 IPQQVRAAGL DIKDVMTDII ENKF

References

[1]

"Evolution of symbiotic bacteria in the distal human intestine." Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C., Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H., Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K., Knight R.D., Gordon J.I. PLoS Biol. 5:1574-1586(2007) [PubMed: 17579514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000139 Genomic DNA. Translation: ABR38257.1.
RefSeq	YP_001297879.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5301516.
GenomeReviews	Gene locus BVU_0547 in contig CP000139_GR.
KEGG	bvu:BVU_0547.
NMPDR	fig|435590.6.peg.524.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00047. [Tree]
InterPro	IPR011761. ATP-grasp. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. [Graphical view]
Pfam	PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DDL_BACV8

Accession

Primary (citable) accession number: A6KXU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 24, 2007
Last modified:	November 25, 2008
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)