A6KWM0 (GLAB1_BACV8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,3-galactosidase B
EC=3.2.1.n1
EC=3.2.1.n2

Alternative name(s):
Exo-alpha-galactosidase B1
EC=3.2.1.22

Gene names

Name:	glaB1
Ordered Locus Names:	BVU_0104

Organism

Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / NCTC 11154) [Complete proteome] [HAMAP]

Taxonomic identifier

435590 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	592 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Alpha-galactosidase. Removes both branched alpha-1,3-linked galactose residues of blood group B antigens and linear alpha-1,3-linked galactose structures By similarity.
Catalytic activity	Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-galactosidic residues, producing free D-galactose. Hydrolysis of terminal, non-reducing linear (1->3)-alpha-D-galactosidic residues, producing free D-galactose. Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.
Subcellular location	Cell membrane; Lipid-anchor Potential.
Sequence similarities	Belongs to the glycosyl hydrolase 110 family. B subfamily. Contains 3 PbH1 repeats.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Lipoprotein Palmitate
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	raffinose alpha-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 14	14	Potential
	Chain	15 – 592	578	Alpha-1,3-galactosidase B		PRO_0000348479
Regions
	Repeat	429 – 451	23	PbH1 1
	Repeat	452 – 474	23	PbH1 2
	Repeat	485 – 538	54	PbH1 3
Amino acid modifications
	Lipidation	15	1	N-palmitoyl cysteine Potential
	Lipidation	15	1	S-diacylglycerol cysteine Potential

Sequences

	Sequence		Length	Mass (Da)
	A6KWM0 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: 836AB742D340C573	FASTA	592	66,484
	10 20 30 40 50 60 MKLLSVLSLS LVLSCTTLSA QKVYEISAFG LKANSSKNAS PVLQKALAKI KAEYKEGEKV 70 80 90 100 110 120 ILRFPEGRYE FHEKGAAVRE YYISNHDQTN PKKVGIALED MKNLTLDGQG SEFVFHGRML 130 140 150 160 170 180 PVSLLRSENC LLKNFSIDFE NPHIAQVKIV ENDPQDGIVF EPAPWVDYRI AKDSIFEAYG 190 200 210 220 230 240 EGWTMRHSWG IAFDGDTKHL VYNTSDIGCP TKGASEVAPR RIHAPGWKDA RLVPGTVVAM 250 260 270 280 290 300 RGWGRPTPGI FLSHDVNTTI ENVKVHYAEG MGLLAQLCEN ITLEKFGVCL KGDADPRYFT 310 320 330 340 350 360 TQADATHFSG CKGKIVSCNG LYEGMMDDAI NVHGTYLKVV KRVDDRTLVG RYMHGQSWGF 370 380 390 400 410 420 EWGCPGDEVQ FIRSNTMELV GKQNKIISIR PYDKEQTEGA REFLITFQEP VDQVINEQSG 430 440 450 460 470 480 FGIENLTWTP EVLFSGNVIR NNRARGSLFS TPRKTIVENN LFDHTSGAAI LLCGDCNGWF 490 500 510 520 530 540 ETGACRHVII RKNRFVNALT NLFQFTNAVI SIYPEIPDLK GQQQYFHGGP EGGIVIEDNE 550 560 570 580 590 FETFDAPILY AKSVDGLVFR NNTIKLNTEY KPFHPNRNRF WLERVTNVTI AE

References

[1]

"Evolution of symbiotic bacteria in the distal human intestine." Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C., Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H., Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K., Knight R.D., Gordon J.I. PLoS Biol. 5:1574-1586(2007) [PubMed: 17579514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8482 / DSM 1447 / NCTC 11154.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000139 Genomic DNA. Translation: ABR37834.1.
RefSeq	YP_001297456.1. NC_009614.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6KWM0.
Protein family/group databases
CAZy	GH110. Glycoside Hydrolase Family 110.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5301074.
GenomeReviews	Gene locus BVU_0104 in contig CP000139_GR.
KEGG	bvu:BVU_0104.
NMPDR	fig|435590.6.peg.103.
PATRIC	21064883. VBIBacVul85104_0108.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	NOG77539.
HOGENOM	HBG345842.
OMA	LTWTPEV.
ProtClustDB	CLSK823424.
Family and domain databases
InterPro	IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 3 hits.
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS51257. PROKAR_LIPOPROTEIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLAB1_BACV8

Accession

Primary (citable) accession number: A6KWM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	July 24, 2007
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program