A6KWL3 (LPXD_BACV8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-3-O-acylglucosamine N-acyltransferase
EC=2.3.1.-

Gene names

Name:	lpxD
Ordered Locus Names:	BVU_0097

Organism

Bacteroides vulgatus (strain ATCC 8482 / DSM 1447 / NCTC 11154) [Complete proteome] [HAMAP]

Taxonomic identifier

435590 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Bacteroidia › Bacteroidales › Bacteroidaceae › Bacteroides

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the N-acylation of UDP-3-O-acylglucosamine using 3-hydroxyacyl-ACP as the acyl donor. Is involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity. HAMAP MF_00523
Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-3-O-acyl-alpha-D-glucosamine = UDP-2,3-diacyl-alpha-D-glucosamine + [acyl-carrier-protein]. HAMAP MF_00523
Pathway	Bacterial outer membrane biogenesis; LPS lipid A biosynthesis. HAMAP MF_00523
Subunit structure	Homotrimer By similarity. HAMAP MF_00523
Sequence similarities	Belongs to the transferase hexapeptide repeat family. LpxD subfamily.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Domain	Repeat
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	transferase activity, transferring acyl groups other than amino-acyl groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	UDP-3-O-acylglucosamine N-acyltransferase HAMAP MF_00523		PRO_1000050925
Sites
	Active site	240	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6KWL3 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: 13A3872DA5A345CA	FASTA	346	36,893
	10 20 30 40 50 60 MEFSAKQIAE YIQGIIVGDE NATVHTFAKI EEGVPGAISF LSNPKYTHYI YDTQSTIVLV 70 80 90 100 110 120 NKDFVPEQEV KATLIKVDNA YESLAKLLTL YEMSKPKKTG IDPLAYVAPT AKLGKDVYIA 130 140 150 160 170 180 PFACVGDGAE IGDNTSLHPH ATVGSHAKVG NNCTLYPHAT IYHDCLVGNH CTLHAGCVIG 190 200 210 220 230 240 ADGFGFAPSP EGYEKIPQIG IAIIEDNVEI GANTCVDRAT MGATIVHKGV KLDNLIQIAH 250 260 270 280 290 300 NVEVGSHTVM ASQVGIAGST KVGEWCMFGG QVGLAGHIKI GDKVGIGAQA GVPGNVKSNE 310 320 330 340 QILGTPAIDA KNFMKSSAVY KKLPEIYTTL NAMQKEIEEL KKQLNK

References

[1]

"Evolution of symbiotic bacteria in the distal human intestine." Xu J., Mahowald M.A., Ley R.E., Lozupone C.A., Hamady M., Martens E.C., Henrissat B., Coutinho P.M., Minx P., Latreille P., Cordum H., Van Brunt A., Kim K., Fulton R.S., Fulton L.A., Clifton S.W., Wilson R.K., Knight R.D., Gordon J.I. PLoS Biol. 5:1574-1586(2007) [PubMed: 17579514] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8482 / DSM 1447 / NCTC 11154.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000139 Genomic DNA. Translation: ABR37827.1.
RefSeq	YP_001297449.1. NC_009614.1.
3D structure databases
ProteinModelPortal	A6KWL3.
ModBase	Search...
Protein-protein interaction databases
STRING	A6KWL3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5301067.
GenomeReviews	Gene locus BVU_0097 in contig CP000139_GR.
KEGG	bvu:BVU_0097.
NMPDR	fig|435590.6.peg.96.
PATRIC	21064869. VBIBacVul85104_0101.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1044.
HOGENOM	HBG469615.
OMA	SYPAKIM.
ProtClustDB	PRK00892.
Family and domain databases
HAMAP	MF_00523. LpxD. [Tree]
InterPro	IPR001451. Hexapep_transf. IPR011004. Trimer_LpxA-like. IPR007691. UDP-3-O_GlcNAc_AcTrfase. IPR020573. UDP_GlcNAc_AcTrfase_non-rep. [Graphical view]
KO	K02536.
Pfam	PF00132. Hexapep. 3 hits. PF04613. LpxD. 1 hit. [Graphical view]
SUPFAM	SSF51161. Trimer_LpxA_like. 1 hit.
TIGRFAMs	TIGR01853. Lipid_A_lpxD. 1 hit.
PROSITE	PS00101. HEXAPEP_TRANSFERASES. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LPXD_BACV8

Accession

Primary (citable) accession number: A6KWL3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 24, 2007
Last modified:	December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program