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A6H8I0

- UBP22_DANRE

UniProt

A6H8I0 - UBP22_DANRE

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Protein

Ubiquitin carboxyl-terminal hydrolase 22

Gene

usp22

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators, where it is required for transcription (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei168 – 1681NucleophilePROSITE-ProRule annotation
Active sitei460 – 4601Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri44 – 10461UBP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW
  2. ubiquitinyl hydrolase activity Source: InterPro
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. chromatin modification Source: UniProtKB-KW
  3. regulation of transcription, DNA-templated Source: UniProtKB-KW
  4. transcription, DNA-templated Source: UniProtKB-KW
  5. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Activator, Chromatin regulator, Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Cell cycle, Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_209587. HATs acetylate histones.

Protein family/group databases

MEROPSiC19.035.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 22 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 22
Ubiquitin thioesterase 22
Ubiquitin-specific-processing protease 22
Gene namesi
Name:usp22
ORF Names:zgc:136342
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
ProteomesiUP000000437: Unplaced

Organism-specific databases

ZFINiZDB-GENE-060512-211. usp22.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 506506Ubiquitin carboxyl-terminal hydrolase 22PRO_0000367511Add
BLAST

Proteomic databases

PRIDEiA6H8I0.

Expressioni

Gene expression databases

BgeeiA6H8I0.

Interactioni

Subunit structurei

Component of some SAGA transcription coactivator-HAT complexes.By similarity

Protein-protein interaction databases

STRINGi7955.ENSDARP00000059142.

Structurei

3D structure databases

ProteinModelPortaliA6H8I0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini159 – 501343USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family. UBP8 subfamily.Curated
Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri44 – 10461UBP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5533.
HOGENOMiHOG000007260.
HOVERGENiHBG058014.
InParanoidiA6H8I0.
KOiK11366.
PhylomeDBiA6H8I0.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: A6H8I0-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSPAGCSHVN GFKVDNWKQN LRVIYQCFVW SGSAETRKRK AKSCICHMCG
60 70 80 90 100
AHLNRLHSCL HCVFFGCFSK KHIHEHAKNK RHNLAIDLLY GGIYCFVCQD
110 120 130 140 150
YIYDKDMEQI AKEEQRKAWK LQGIGEKYSM WEPTKRELEL LRHNPKRRKI
160 170 180 190 200
TANCTIGLRG LINLGNTCFM NCIVQALTHT PLLRDFFLSD RHKCEMQSNS
210 220 230 240 250
CLVCEMSQLF QEFYSGHRSP HIPFRLLHLV WTHARHLAGY EQQDAHEFLI
260 270 280 290 300
AALDVLHRHC KDDNGKKANN PNHCNCIIDQ IFTGGLQSDV TCQVCHGVST
310 320 330 340 350
TIDPFWDISL DLPGSSTPFW PLSPGSDGSV VNGDSHPSGA TTLTDCLRRF
360 370 380 390 400
TRPEHLGSSA KIKCSGCHSY QESTKQLTMK RLPIVACFHL KRFEHSAKLR
410 420 430 440 450
RKITTYVSFP LELDMTPFMA SSKESRMNGQ YQQPVDSLNN DNKYSLFAVV
460 470 480 490 500
NHQGTLESGH YTTFIRQHKD QWFKCDDAII TKASIKDVLD SEGYLLFYHK

QFLEYE
Length:506
Mass (Da):58,120
Last modified:July 24, 2007 - v1
Checksum:iD749E025E4A15C03
GO
Isoform 2 (identifier: A6H8I0-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     261-261: K → KG
     392-506: RFEHSAKLRR...FYHKQFLEYE → VRAELLLSVLIFQHPLNVFVCFSAV

Note: No experimental confirmation available.

Show »
Length:417
Mass (Da):47,414
Checksum:i72BF3882D515C79F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti90 – 901Y → H in AAI16509. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei261 – 2611K → KG in isoform 2. 1 PublicationVSP_036726
Alternative sequencei392 – 506115RFEHS…FLEYE → VRAELLLSVLIFQHPLNVFV CFSAV in isoform 2. 1 PublicationVSP_036727Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC116508 mRNA. Translation: AAI16509.1.
BC146618 mRNA. Translation: AAI46619.1.
RefSeqiNP_001038713.1. NM_001045248.1.
UniGeneiDr.87145.

Genome annotation databases

GeneIDi692275.
KEGGidre:692275.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC116508 mRNA. Translation: AAI16509.1 .
BC146618 mRNA. Translation: AAI46619.1 .
RefSeqi NP_001038713.1. NM_001045248.1.
UniGenei Dr.87145.

3D structure databases

ProteinModelPortali A6H8I0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 7955.ENSDARP00000059142.

Protein family/group databases

MEROPSi C19.035.

Proteomic databases

PRIDEi A6H8I0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 692275.
KEGGi dre:692275.

Organism-specific databases

CTDi 23326.
ZFINi ZDB-GENE-060512-211. usp22.

Phylogenomic databases

eggNOGi COG5533.
HOGENOMi HOG000007260.
HOVERGENi HBG058014.
InParanoidi A6H8I0.
KOi K11366.
PhylomeDBi A6H8I0.

Enzyme and pathway databases

Reactomei REACT_209587. HATs acetylate histones.

Miscellaneous databases

NextBioi 20902479.
PROi A6H8I0.

Gene expression databases

Bgeei A6H8I0.

Family and domain databases

Gene3Di 3.30.40.10. 1 hit.
InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
PROSITEi PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: Embryo and Eye.

Entry informationi

Entry nameiUBP22_DANRE
AccessioniPrimary (citable) accession number: A6H8I0
Secondary accession number(s): Q1JQ28
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 24, 2007
Last modified: November 26, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3