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A6H8I0

- UBP22_DANRE

UniProt

A6H8I0 - UBP22_DANRE

Protein

Ubiquitin carboxyl-terminal hydrolase 22

Gene

usp22

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (24 Jul 2007)
      Previous versions | rss
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    Functioni

    Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators, where it is required for transcription By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei168 – 1681NucleophilePROSITE-ProRule annotation
    Active sitei460 – 4601Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri44 – 10461UBP-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. ubiquitinyl hydrolase activity Source: InterPro
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. chromatin modification Source: UniProtKB-KW
    3. regulation of transcription, DNA-templated Source: UniProtKB-KW
    4. transcription, DNA-templated Source: UniProtKB-KW
    5. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Activator, Chromatin regulator, Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Cell cycle, Transcription, Transcription regulation, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiC19.035.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 22 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 22
    Ubiquitin thioesterase 22
    Ubiquitin-specific-processing protease 22
    Gene namesi
    Name:usp22
    ORF Names:zgc:136342
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Unplaced

    Organism-specific databases

    ZFINiZDB-GENE-060512-211. usp22.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 506506Ubiquitin carboxyl-terminal hydrolase 22PRO_0000367511Add
    BLAST

    Proteomic databases

    PRIDEiA6H8I0.

    Expressioni

    Gene expression databases

    BgeeiA6H8I0.

    Interactioni

    Subunit structurei

    Component of some SAGA transcription coactivator-HAT complexes.By similarity

    Protein-protein interaction databases

    STRINGi7955.ENSDARP00000059142.

    Structurei

    3D structure databases

    ProteinModelPortaliA6H8I0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini159 – 501343USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family. UBP8 subfamily.Curated
    Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri44 – 10461UBP-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5533.
    HOGENOMiHOG000007260.
    HOVERGENiHBG058014.
    KOiK11366.
    PhylomeDBiA6H8I0.

    Family and domain databases

    Gene3Di3.30.40.10. 1 hit.
    InterProiIPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view]
    PfamiPF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view]
    PROSITEiPS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: A6H8I0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSPAGCSHVN GFKVDNWKQN LRVIYQCFVW SGSAETRKRK AKSCICHMCG    50
    AHLNRLHSCL HCVFFGCFSK KHIHEHAKNK RHNLAIDLLY GGIYCFVCQD 100
    YIYDKDMEQI AKEEQRKAWK LQGIGEKYSM WEPTKRELEL LRHNPKRRKI 150
    TANCTIGLRG LINLGNTCFM NCIVQALTHT PLLRDFFLSD RHKCEMQSNS 200
    CLVCEMSQLF QEFYSGHRSP HIPFRLLHLV WTHARHLAGY EQQDAHEFLI 250
    AALDVLHRHC KDDNGKKANN PNHCNCIIDQ IFTGGLQSDV TCQVCHGVST 300
    TIDPFWDISL DLPGSSTPFW PLSPGSDGSV VNGDSHPSGA TTLTDCLRRF 350
    TRPEHLGSSA KIKCSGCHSY QESTKQLTMK RLPIVACFHL KRFEHSAKLR 400
    RKITTYVSFP LELDMTPFMA SSKESRMNGQ YQQPVDSLNN DNKYSLFAVV 450
    NHQGTLESGH YTTFIRQHKD QWFKCDDAII TKASIKDVLD SEGYLLFYHK 500
    QFLEYE 506
    Length:506
    Mass (Da):58,120
    Last modified:July 24, 2007 - v1
    Checksum:iD749E025E4A15C03
    GO
    Isoform 2 (identifier: A6H8I0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         261-261: K → KG
         392-506: RFEHSAKLRR...FYHKQFLEYE → VRAELLLSVLIFQHPLNVFVCFSAV

    Note: No experimental confirmation available.

    Show »
    Length:417
    Mass (Da):47,414
    Checksum:i72BF3882D515C79F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti90 – 901Y → H in AAI16509. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei261 – 2611K → KG in isoform 2. 1 PublicationVSP_036726
    Alternative sequencei392 – 506115RFEHS…FLEYE → VRAELLLSVLIFQHPLNVFV CFSAV in isoform 2. 1 PublicationVSP_036727Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC116508 mRNA. Translation: AAI16509.1.
    BC146618 mRNA. Translation: AAI46619.1.
    RefSeqiNP_001038713.1. NM_001045248.1.
    UniGeneiDr.87145.

    Genome annotation databases

    GeneIDi692275.
    KEGGidre:692275.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC116508 mRNA. Translation: AAI16509.1 .
    BC146618 mRNA. Translation: AAI46619.1 .
    RefSeqi NP_001038713.1. NM_001045248.1.
    UniGenei Dr.87145.

    3D structure databases

    ProteinModelPortali A6H8I0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7955.ENSDARP00000059142.

    Protein family/group databases

    MEROPSi C19.035.

    Proteomic databases

    PRIDEi A6H8I0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 692275.
    KEGGi dre:692275.

    Organism-specific databases

    CTDi 23326.
    ZFINi ZDB-GENE-060512-211. usp22.

    Phylogenomic databases

    eggNOGi COG5533.
    HOGENOMi HOG000007260.
    HOVERGENi HBG058014.
    KOi K11366.
    PhylomeDBi A6H8I0.

    Miscellaneous databases

    NextBioi 20902479.
    PROi A6H8I0.

    Gene expression databases

    Bgeei A6H8I0.

    Family and domain databases

    Gene3Di 3.30.40.10. 1 hit.
    InterProi IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view ]
    Pfami PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view ]
    PROSITEi PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Zebrafish Gene Collection (ZGC) project
      Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Tissue: Embryo and Eye.

    Entry informationi

    Entry nameiUBP22_DANRE
    AccessioniPrimary (citable) accession number: A6H8I0
    Secondary accession number(s): Q1JQ28
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: July 24, 2007
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3