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A6H8I0 (UBP22_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 22

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 22
Ubiquitin thioesterase 22
Ubiquitin-specific-processing protease 22
Gene names
Name:usp22
ORF Names:zgc:136342
OrganismDanio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]
Taxonomic identifier7955 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Protein attributes

Sequence length506 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. Catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. Recruited to specific gene promoters by activators, where it is required for transcription By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Component of some SAGA transcription coactivator-HAT complexes By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the peptidase C19 family. UBP8 subfamily.

Contains 1 UBP-type zinc finger.

Contains 1 USP domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: A6H8I0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: A6H8I0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     261-261: K → KG
     392-506: RFEHSAKLRR...FYHKQFLEYE → VRAELLLSVLIFQHPLNVFVCFSAV
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 506506Ubiquitin carboxyl-terminal hydrolase 22
PRO_0000367511

Regions

Domain159 – 501343USP
Zinc finger44 – 10461UBP-type

Sites

Active site1681Nucleophile By similarity
Active site4601Proton acceptor By similarity

Natural variations

Alternative sequence2611K → KG in isoform 2.
VSP_036726
Alternative sequence392 – 506115RFEHS…FLEYE → VRAELLLSVLIFQHPLNVFV CFSAV in isoform 2.
VSP_036727

Experimental info

Sequence conflict901Y → H in AAI16509. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 24, 2007. Version 1.
Checksum: D749E025E4A15C03

FASTA50658,120
        10         20         30         40         50         60 
MSPAGCSHVN GFKVDNWKQN LRVIYQCFVW SGSAETRKRK AKSCICHMCG AHLNRLHSCL 

        70         80         90        100        110        120 
HCVFFGCFSK KHIHEHAKNK RHNLAIDLLY GGIYCFVCQD YIYDKDMEQI AKEEQRKAWK 

       130        140        150        160        170        180 
LQGIGEKYSM WEPTKRELEL LRHNPKRRKI TANCTIGLRG LINLGNTCFM NCIVQALTHT 

       190        200        210        220        230        240 
PLLRDFFLSD RHKCEMQSNS CLVCEMSQLF QEFYSGHRSP HIPFRLLHLV WTHARHLAGY 

       250        260        270        280        290        300 
EQQDAHEFLI AALDVLHRHC KDDNGKKANN PNHCNCIIDQ IFTGGLQSDV TCQVCHGVST 

       310        320        330        340        350        360 
TIDPFWDISL DLPGSSTPFW PLSPGSDGSV VNGDSHPSGA TTLTDCLRRF TRPEHLGSSA 

       370        380        390        400        410        420 
KIKCSGCHSY QESTKQLTMK RLPIVACFHL KRFEHSAKLR RKITTYVSFP LELDMTPFMA 

       430        440        450        460        470        480 
SSKESRMNGQ YQQPVDSLNN DNKYSLFAVV NHQGTLESGH YTTFIRQHKD QWFKCDDAII 

       490        500 
TKASIKDVLD SEGYLLFYHK QFLEYE 

« Hide

Isoform 2 [UniParc].

Checksum: 72BF3882D515C79F
Show »

FASTA41747,414

References

[1]NIH - Zebrafish Gene Collection (ZGC) project
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Embryo and Eye.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC116508 mRNA. Translation: AAI16509.1.
BC146618 mRNA. Translation: AAI46619.1.
RefSeqNP_001038713.1. NM_001045248.1.
UniGeneDr.87145.

3D structure databases

ProteinModelPortalA6H8I0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING7955.ENSDARP00000059142.

Protein family/group databases

MEROPSC19.035.

Proteomic databases

PRIDEA6H8I0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID692275.
KEGGdre:692275.

Organism-specific databases

CTD23326.
ZFINZDB-GENE-060512-211. usp22.

Phylogenomic databases

eggNOGCOG5533.
HOGENOMHOG000007260.
HOVERGENHBG058014.
KOK11366.
PhylomeDBA6H8I0.

Gene expression databases

BgeeA6H8I0.

Family and domain databases

Gene3D3.30.40.10. 1 hit.
InterProIPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamPF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
PROSITEPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20902479.
PROA6H8I0.

Entry information

Entry nameUBP22_DANRE
AccessionPrimary (citable) accession number: A6H8I0
Secondary accession number(s): Q1JQ28
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 24, 2007
Last modified: April 16, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries