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Protein

DENN domain-containing protein 4C

Gene

Dennd4c

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide exchange factor (GEF) activating RAB10. Promotes the exchange of GDP to GTP, converting inactive GDP-bound RAB10 into its active GTP-bound form. Thereby, stimulates SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane in response to insulin.1 Publication

GO - Molecular functioni

  • Rab guanyl-nucleotide exchange factor activity Source: UniProtKB

GO - Biological processi

  • cellular response to insulin stimulus Source: UniProtKB
  • protein localization to plasma membrane Source: UniProtKB
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
DENN domain-containing protein 4C
Gene namesi
Name:Dennd4c
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1914769. Dennd4c.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  • cytosol Source: UniProtKB
  • insulin-responsive compartment Source: UniProtKB
  • plasma membrane Source: UniProtKB
  • retromer complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004204461 – 1906DENN domain-containing protein 4CAdd BLAST1906

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei702PhosphoserineBy similarity1
Modified residuei736PhosphoserineBy similarity1
Modified residuei740PhosphoserineCombined sources1
Modified residuei966PhosphothreonineCombined sources1
Modified residuei971PhosphoserineCombined sources1
Modified residuei973PhosphothreonineBy similarity1
Modified residuei987PhosphoserineCombined sources1
Modified residuei1000PhosphoserineBy similarity1
Modified residuei1043Phosphoserine1 Publication1
Modified residuei1058PhosphoserineBy similarity1
Modified residuei1096PhosphoserineCombined sources1 Publication1
Modified residuei1123PhosphoserineBy similarity1
Modified residuei1181PhosphoserineBy similarity1
Modified residuei1221PhosphoserineBy similarity1
Modified residuei1240PhosphoserineBy similarity1
Modified residuei1248PhosphoserineCombined sources1
Modified residuei1274PhosphoserineBy similarity1
Modified residuei1321Phosphoserine1 Publication1
Modified residuei1333PhosphoserineBy similarity1
Modified residuei1342PhosphoserineBy similarity1
Modified residuei1620PhosphoserineBy similarity1
Modified residuei1624PhosphoserineBy similarity1
Modified residuei1626PhosphoserineBy similarity1
Modified residuei1637PhosphoserineCombined sources1
Modified residuei1796PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated in response to insulin.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiA6H8H2.
PaxDbiA6H8H2.
PRIDEiA6H8H2.

PTM databases

iPTMnetiA6H8H2.
PhosphoSitePlusiA6H8H2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000038024.
ExpressionAtlasiA6H8H2. baseline and differential.
GenevisibleiA6H8H2. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000039860.

Structurei

3D structure databases

ProteinModelPortaliA6H8H2.
SMRiA6H8H2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 199MABPPROSITE-ProRule annotationAdd BLAST160
Domaini202 – 275uDENNPROSITE-ProRule annotationAdd BLAST74
Domaini307 – 491DENNPROSITE-ProRule annotationAdd BLAST185
Domaini557 – 631dDENNPROSITE-ProRule annotationAdd BLAST75
Repeati818 – 852PPRAdd BLAST35

Sequence similaritiesi

Contains 1 dDENN domain.PROSITE-ProRule annotation
Contains 1 DENN domain.PROSITE-ProRule annotation
Contains 1 MABP domain.PROSITE-ProRule annotation
Contains 1 uDENN domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2127. Eukaryota.
ENOG410XQMX. LUCA.
GeneTreeiENSGT00760000118819.
HOGENOMiHOG000231590.
HOVERGENiHBG058313.
InParanoidiA6H8H2.
KOiK20163.
OMAiGMKQAAN.
OrthoDBiEOG091G00CV.
TreeFamiTF313237.

Family and domain databases

InterProiIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR023341. MABP.
IPR005113. uDENN_dom.
[Graphical view]
PfamiPF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
PF03456. uDENN. 1 hit.
[Graphical view]
SMARTiSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00800. uDENN. 1 hit.
[Graphical view]
PROSITEiPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS51498. MABP. 1 hit.
PS50946. UDENN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A6H8H2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIEDKGPRVT DYFVVAGLTD TSTLLDQEIN RTDTNSIGPK APITDIAVII
60 70 80 90 100
KSAGETVPEG YTCVEATPSA LQANLNYGSL KSPELFLCYR RGRDKPPLTD
110 120 130 140 150
IGVLYEGKER LMPGCEVIQA TPYGRCANVN NSSTTSQRIF ITYRRAPPVR
160 170 180 190 200
SQNSLAVTDI CVIITSKGET PPHTFCKVDK NLNCGMWGSN VFLCYKKSVP
210 220 230 240 250
ASNAIAYKAG LIFRYPEEDY ESFPLSPSVP LFCLPMGATI ECWDPQIKYP
260 270 280 290 300
LPVFSTFVLT GSSAEKVYGA AIQFYEPYSQ ERLTEKQLTQ LGLLTLVEKR
310 320 330 340 350
VVSKPINSNK CICLLSHWPF FEAFKNFLMF IYKVSVSGPH PLPIEKHISH
360 370 380 390 400
FMQNIPFPSP QRPRILIQLS VHDAFILSQP VSTPLPLSGA NFSSLLMNLG
410 420 430 440 450
PENCATLLLL VLLESKILLH SLRPAVLTGV AEAVVAMIFP FQWQCPYIPL
460 470 480 490 500
CPLSLAGVLS APLPFIVGVD SRYFDLHDPP QDVVCIDLDT NTLYVADERK
510 520 530 540 550
NINWKQLPKR PCKSLLGTLR RLYQQLCSVH RKPQESSAIE MTPIEADYSW
560 570 580 590 600
QKKMTQLEME IQETFLRFMA SILKGYRSYL RPITEAPSNK ATAADSLFDR
610 620 630 640 650
QGFLKSRDRA YTKFYTLLSK TQIFIRFIEE CSFVSDKDTG LAFFDDCIEK
660 670 680 690 700
LFPDKGVERT EKVDLDSAED TRLIELDDSQ RSEHTVFIMP PEPPPDDGNN
710 720 730 740 750
LSPQYSYTYF PRLDLKLFDS PQKLKLCFNR HPPGSSITNS PALMAKRTKQ
760 770 780 790 800
EIKTAHKLAK RCYTNPPQWA KYLFSHCYSL WFICLPAYVR VSHPKVRALQ
810 820 830 840 850
QAHDVLVKMR KTDVDPLDEV CYRVVMQLCG LWVNPVLAVR VLFEMKTARI
860 870 880 890 900
KPNAITYGYY NKVVLESPWP SSTRSGIFLW TKVRNVVHGL AQFRQPLKKT
910 920 930 940 950
GQKSQVFSIS GGQSDQGYGS KDELVKEGAD GHAPEEHTPP ELTTTELHIE
960 970 980 990 1000
EECDISAIVS KHLQPTPEPQ SPTEPPAWGS SIVKVPSGLF DTNNRTSTGS
1010 1020 1030 1040 1050
TSTVLFSTQA PVEDAVFSEV TNFKKNGDRG EKKQKHFPER SCSFSSESRA
1060 1070 1080 1090 1100
GMLLKKSSLD LNSSEMAIMM GADAKILTAA LTCPKTSPPH VTRTHSFENV
1110 1120 1130 1140 1150
NCHLADSRTR MSEGTRDSEH RSSPVLEMLE ESQELLEPVV GDNVAETAAE
1160 1170 1180 1190 1200
MTCNSLQSNS HSDQSRDTQA GAQDPVNKRS SSYATRKAIE REDVETGLDP
1210 1220 1230 1240 1250
LSLLATECVE KTSDSEDKLF SPVISRNLAD EIESYMNLKS PLGSKSCSME
1260 1270 1280 1290 1300
LHGEGNQEPG SPAVFAHPLE RSSSLPSDRG PPARDSTETE KSSPAVSSSK
1310 1320 1330 1340 1350
TLTGRFKPQS PYRAYKDRST SLSALVRSSP NSSLGSVVNS LSGLKLDNIL
1360 1370 1380 1390 1400
SGPKIDVLKS SMKQAATVAS KMWVAVASAY SYSDDEEETN KDYSFPAGLE
1410 1420 1430 1440 1450
DHHIVGETLS PNTSVSGLVP SELTQSNTSL GSSSSSGDVG KLQCPAGEVP
1460 1470 1480 1490 1500
FSRNIKGQDF EKSDHGSSQN TSMSSIYQNC AMEVLMSSCS QCRACGALVY
1510 1520 1530 1540 1550
DEEIMAGWTA DDSNLNTTCP FCKSNFLPLL NVEFKDLRGS ASFFLKPSTS
1560 1570 1580 1590 1600
GDSLQSGSIP SASEPSEHKP TSSSAEPDLI SFMDFSKHSE TITEEASYTV
1610 1620 1630 1640 1650
ESSDEIKKTN GDVQSVKMSS VPNSLSKRNV SLTRSHSVGG PLQNIDFSQR
1660 1670 1680 1690 1700
PFHGVSTVSL PSSLQEDVDH LGKRPSPPPV SVPYLSPLVL RKELESLLEN
1710 1720 1730 1740 1750
EGDQVIHTSS FINQHPIIFW NLVWYFRRLD LPSNLPGLIL TSEHCNGGVQ
1760 1770 1780 1790 1800
LPLSSLSQDS KLVYIQLLWD NINLHQEPGE PLYVSWRNLN SEKKPSLLSE
1810 1820 1830 1840 1850
QQQAASALVE TIRQSIQQND VLKPINLLSQ QMKPGTKRQR SLYREILFLS
1860 1870 1880 1890 1900
LVSLGRENID IEAFDNEYGL AYRSLPSESL ERLQRIDAPP SISVEWCRKC

FGAPLI
Length:1,906
Mass (Da):211,458
Last modified:July 24, 2007 - v1
Checksum:i705904CB0F5BED64
GO
Isoform 2 (identifier: A6H8H2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     910-910: S → SAPQNAACGSDGDTVSHGSVDSSNDANNGEHTVFVRDLISLDSIDNHSST

Show »
Length:1,955
Mass (Da):216,455
Checksum:i3D97B5B382949DFF
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_044491910S → SAPQNAACGSDGDTVSHGSV DSSNDANNGEHTVFVRDLIS LDSIDNHSST in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL772228, BX005084 Genomic DNA. Translation: CAM18278.1.
BX005084, AL772228 Genomic DNA. Translation: CAM27931.1.
BC146602 mRNA. Translation: AAI46603.1.
AK155220 mRNA. Translation: BAE33131.1.
CCDSiCCDS51218.2. [A6H8H2-1]
RefSeqiNP_908976.1. NM_184088.1. [A6H8H2-1]
XP_006538085.1. XM_006538022.2. [A6H8H2-2]
XP_006538086.1. XM_006538023.3. [A6H8H2-2]
UniGeneiMm.273718.
Mm.472357.

Genome annotation databases

EnsembliENSMUST00000082026; ENSMUSP00000080685; ENSMUSG00000038024. [A6H8H2-2]
ENSMUST00000142837; ENSMUSP00000123367; ENSMUSG00000038024. [A6H8H2-1]
GeneIDi329877.
KEGGimmu:329877.
UCSCiuc008tmb.2. mouse. [A6H8H2-1]
uc008tmc.1. mouse. [A6H8H2-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL772228, BX005084 Genomic DNA. Translation: CAM18278.1.
BX005084, AL772228 Genomic DNA. Translation: CAM27931.1.
BC146602 mRNA. Translation: AAI46603.1.
AK155220 mRNA. Translation: BAE33131.1.
CCDSiCCDS51218.2. [A6H8H2-1]
RefSeqiNP_908976.1. NM_184088.1. [A6H8H2-1]
XP_006538085.1. XM_006538022.2. [A6H8H2-2]
XP_006538086.1. XM_006538023.3. [A6H8H2-2]
UniGeneiMm.273718.
Mm.472357.

3D structure databases

ProteinModelPortaliA6H8H2.
SMRiA6H8H2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000039860.

PTM databases

iPTMnetiA6H8H2.
PhosphoSitePlusiA6H8H2.

Proteomic databases

MaxQBiA6H8H2.
PaxDbiA6H8H2.
PRIDEiA6H8H2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000082026; ENSMUSP00000080685; ENSMUSG00000038024. [A6H8H2-2]
ENSMUST00000142837; ENSMUSP00000123367; ENSMUSG00000038024. [A6H8H2-1]
GeneIDi329877.
KEGGimmu:329877.
UCSCiuc008tmb.2. mouse. [A6H8H2-1]
uc008tmc.1. mouse. [A6H8H2-2]

Organism-specific databases

CTDi55667.
MGIiMGI:1914769. Dennd4c.

Phylogenomic databases

eggNOGiKOG2127. Eukaryota.
ENOG410XQMX. LUCA.
GeneTreeiENSGT00760000118819.
HOGENOMiHOG000231590.
HOVERGENiHBG058313.
InParanoidiA6H8H2.
KOiK20163.
OMAiGMKQAAN.
OrthoDBiEOG091G00CV.
TreeFamiTF313237.

Miscellaneous databases

PROiA6H8H2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038024.
ExpressionAtlasiA6H8H2. baseline and differential.
GenevisibleiA6H8H2. MM.

Family and domain databases

InterProiIPR005112. dDENN_dom.
IPR001194. DENN_dom.
IPR023341. MABP.
IPR005113. uDENN_dom.
[Graphical view]
PfamiPF03455. dDENN. 1 hit.
PF02141. DENN. 1 hit.
PF03456. uDENN. 1 hit.
[Graphical view]
SMARTiSM00801. dDENN. 1 hit.
SM00799. DENN. 1 hit.
SM00800. uDENN. 1 hit.
[Graphical view]
PROSITEiPS50947. DDENN. 1 hit.
PS50211. DENN. 1 hit.
PS51498. MABP. 1 hit.
PS50946. UDENN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDEN4C_MOUSE
AccessioniPrimary (citable) accession number: A6H8H2
Secondary accession number(s): A2AJX5, Q3U2K9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: July 24, 2007
Last modified: November 2, 2016
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.