A6H7E1 (SYMM_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase, mitochondrial
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase 2
Mitochondrial methionyl-tRNA synthetase
Short name=MtMetRS

Gene names

Name:

MARS2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	593 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 29	29	Mitochondrion Potential
	Chain	30 – 593	564	Methionine--tRNA ligase, mitochondrial		PRO_0000328569
Regions
	Motif	52 – 62	11	"HIGH" region
	Motif	347 – 351	5	"KMSKS" region
Sites
	Binding site	350	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6H7E1 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: 1B2593DC8AA40F1C	FASTA	593	66,648
	10 20 30 40 50 60 MLRVSAFRLL GRRGASRVSL LEDFSFRYYS SGPLGVRDDT RDSRAYFTTP IFYVNAAPHI 70 80 90 100 110 120 GHLYSALLAD ALCRHHRLRV PSDAATGFST GTDEHGLKIQ QAAAAAGLAP SELCDRVSAQ 130 140 150 160 170 180 FQQLFREADI SSTDFIRTTE ARHRIAVQHF WGMLKSRGLL YKGLYEGWYC ASDECFLPEA 190 200 210 220 230 240 KVTRQPGPSG DLCPVSLESG HPVSWTKEEN YIFRLSQFRE PLQQWLRGDP QAITPEPFHH 250 260 270 280 290 300 TVLQWLEEEL PDLSVSRRSS HLHWGIPVPG DDSQTIYVWL DALVNYLTVV GYPDAEFKSW 310 320 330 340 350 360 WPNTSHIIGK DILKFHAIYW PALLLGAGMS PPHRIYVHSH WTVCGQKMSK SLGNVVDPRT 370 380 390 400 410 420 CLDRYTVDGF RYFLLRQGVP SWDCDYYDEK VVKLLDSELA DALGGLLNRC TANKINPSGI 430 440 450 460 470 480 YPAFCATCFP SEPGLVGPSG RAQAEDYALV SAVATLPKQV ADHYDNFQIY KALEAVSSCV 490 500 510 520 530 540 RQTNGFVQRH APWKLNWESP VDAPWLGTVL HVALECLRVF GTLLQPVTPS LADRLLSRLG 550 560 570 580 590 VSSTERSLGE LHFLSRFYGH PSPFEGRRLG PETGVLFPRL DQSRSWLVKA HKT

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal skin.
[2]	"Characterization of 954 bovine full-CDS cDNA sequences." Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L. BMC Genomics 6:166-166(2005) [PubMed: 16305752] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-593.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC146211 mRNA. Translation: AAI46212.1. BT026161 mRNA. Translation: ABG67000.1.
IPI	IPI00729326.
RefSeq	NP_001092441.1. NM_001098971.1.
UniGene	Bt.16783.
3D structure databases
ProteinModelPortal	A6H7E1.
ModBase	Search...
Protein-protein interaction databases
STRING	A6H7E1.
Proteomic databases
PRIDE	A6H7E1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000002662; ENSBTAP00000042279; ENSBTAG00000002056.
GeneID	514726.
KEGG	bta:514726.
Organism-specific databases
CTD	92935.
Phylogenomic databases
eggNOG	maNOG08781.
GeneTree	ENSGT00550000075136.
HOVERGEN	HBG080702.
InParanoid	A6H7E1.
OMA	GTYPVFC.
OrthoDB	EOG4T7830.
Family and domain databases
InterPro	IPR015413. aa-tRNA-synt_I. IPR014758. Met-tRNA_synth. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01874.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. MetG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

SYMM_BOVIN

Accession

Primary (citable) accession number: A6H7E1
Secondary accession number(s): Q0V8Q7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 8, 2008
Last sequence update:	July 24, 2007
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program