Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A6H772

- PP4C_BOVIN

UniProt

A6H772 - PP4C_BOVIN

Protein

Serine/threonine-protein phosphatase 4 catalytic subunit

Gene

PPP4C

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (24 Jul 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase. In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.By similarity

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi54 – 541Manganese 1By similarity
    Metal bindingi56 – 561Manganese 1By similarity
    Metal bindingi82 – 821Manganese 1By similarity
    Metal bindingi82 – 821Manganese 2By similarity
    Metal bindingi114 – 1141Manganese 2By similarity
    Active sitei115 – 1151Proton donorBy similarity
    Metal bindingi164 – 1641Manganese 2By similarity
    Metal bindingi238 – 2381Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein serine/threonine phosphatase activity Source: UniProtKB

    GO - Biological processi

    1. dephosphorylation Source: GOC
    2. protein phosphorylation Source: UniProtKB
    3. regulation of double-strand break repair via homologous recombination Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase 4 catalytic subunit (EC:3.1.3.16)
    Short name:
    PP4C
    Short name:
    Pp4
    Gene namesi
    Name:PPP4C
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 25

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. microtubule organizing center Source: UniProtKB-SubCell
    3. nucleus Source: UniProtKB-SubCell
    4. plasma membrane Source: Ensembl
    5. protein phosphatase 4 complex Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 307306Serine/threonine-protein phosphatase 4 catalytic subunitPRO_0000307863Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei307 – 3071Leucine methyl esterBy similarity

    Keywords - PTMi

    Acetylation, Methylation

    Proteomic databases

    PRIDEiA6H772.

    Interactioni

    Subunit structurei

    Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits By similarity. Component of the PP4 complexes PPP4C-PPP4R1, PPP4C-PPP4R2, PPP4C-PPP4R2-PPP4R3A, PPP4C-PPP4R2-PPP4R3B and PPP4C-PPP4R4 By similarity. The PPP4C-PPP4R2 complex appears to be a tetramer composed of 2 molecules of PPP4C and 2 molecules of PPP4R2 By similarity. Interacts with REL, NFKB1/p50 and RELA By similarity. Interacts with SMN1 AND GEMIN4. Interacts with IRS4 (phosphorylated) By similarity. Interacts with SMEK1/PPP4R3A; the interaction requires PP4R2 By similarity. Interacts with HDAC3 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PPP4R1F1MRS13EBI-2638542,EBI-2638538

    Protein-protein interaction databases

    IntActiA6H772. 1 interaction.
    STRINGi9913.ENSBTAP00000017178.

    Structurei

    3D structure databases

    ProteinModelPortaliA6H772.
    SMRiA6H772. Positions 6-291.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0639.
    GeneTreeiENSGT00550000074618.
    HOGENOMiHOG000172696.
    HOVERGENiHBG000216.
    InParanoidiA6H772.
    KOiK15423.
    OMAiVFNHRND.
    OrthoDBiEOG74N5H2.
    TreeFamiTF105559.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A6H772-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEISDLDRQ IEQLRRCELI KESEVKALCA KAREILVEES NVQRVDSPVT    50
    VCGDIHGQFY DLKELFRVGG DVPETNYLFM GDFVDRGFYS VETFLLLLAL 100
    KVRYPDRITL IRGNHESRQI TQVYGFYDEC LRKYGSVTVW RYCTEIFDYL 150
    SLSAIIDGKI FCVHGGLSPS IQTLDQIRTI DRKQEVPHDG PMCDLLWSDP 200
    EDTTGWGVSP RGAGYLFGSD VVAQFNAAND IDMICRAHQL VMEGYKWHFN 250
    ETVLTVWSAP NYCYRCGNVA AILELDEHLQ KDFIIFEAAP QETRGIPSKK 300
    PVADYFL 307
    Length:307
    Mass (Da):35,080
    Last modified:July 24, 2007 - v1
    Checksum:iD6FE470A5C6CBCAC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC146137 mRNA. Translation: AAI46138.1.
    RefSeqiNP_001092578.1. NM_001099108.2.
    XP_005225004.1. XM_005224947.1.
    XP_005225005.1. XM_005224948.1.
    UniGeneiBt.42027.

    Genome annotation databases

    EnsembliENSBTAT00000017178; ENSBTAP00000017178; ENSBTAG00000012928.
    GeneIDi540398.
    KEGGibta:540398.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC146137 mRNA. Translation: AAI46138.1 .
    RefSeqi NP_001092578.1. NM_001099108.2.
    XP_005225004.1. XM_005224947.1.
    XP_005225005.1. XM_005224948.1.
    UniGenei Bt.42027.

    3D structure databases

    ProteinModelPortali A6H772.
    SMRi A6H772. Positions 6-291.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi A6H772. 1 interaction.
    STRINGi 9913.ENSBTAP00000017178.

    Proteomic databases

    PRIDEi A6H772.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000017178 ; ENSBTAP00000017178 ; ENSBTAG00000012928 .
    GeneIDi 540398.
    KEGGi bta:540398.

    Organism-specific databases

    CTDi 5531.

    Phylogenomic databases

    eggNOGi COG0639.
    GeneTreei ENSGT00550000074618.
    HOGENOMi HOG000172696.
    HOVERGENi HBG000216.
    InParanoidi A6H772.
    KOi K15423.
    OMAi VFNHRND.
    OrthoDBi EOG74N5H2.
    TreeFami TF105559.

    Miscellaneous databases

    NextBioi 20878609.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal lung.

    Entry informationi

    Entry nameiPP4C_BOVIN
    AccessioniPrimary (citable) accession number: A6H772
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 23, 2007
    Last sequence update: July 24, 2007
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3