SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A6H772

- PP4C_BOVIN

UniProt

A6H772 - PP4C_BOVIN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Serine/threonine-protein phosphatase 4 catalytic subunit

Gene
PPP4C
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration By similarity. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3 By similarity. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair By similarity. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase By similarity. In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin By similarity.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Binds 2 manganese ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541Manganese 1 By similarity
Metal bindingi56 – 561Manganese 1 By similarity
Metal bindingi82 – 821Manganese 1 By similarity
Metal bindingi82 – 821Manganese 2 By similarity
Metal bindingi114 – 1141Manganese 2 By similarity
Active sitei115 – 1151Proton donor By similarity
Metal bindingi164 – 1641Manganese 2 By similarity
Metal bindingi238 – 2381Manganese 2 By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. protein binding Source: IntAct
  3. protein serine/threonine phosphatase activity Source: UniProtKB

GO - Biological processi

  1. dephosphorylation Source: GOC
  2. protein phosphorylation Source: UniProtKB
  3. regulation of double-strand break repair via homologous recombination Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase 4 catalytic subunit (EC:3.1.3.16)
Short name:
PP4C
Short name:
Pp4
Gene namesi
Name:PPP4C
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 25

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. microtubule organizing center Source: UniProtKB-SubCell
  3. nucleus Source: UniProtKB-SubCell
  4. plasma membrane Source: Ensembl
  5. protein phosphatase 4 complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 307306Serine/threonine-protein phosphatase 4 catalytic subunitPRO_0000307863Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei307 – 3071Leucine methyl ester By similarity

Keywords - PTMi

Acetylation, Methylation

Proteomic databases

PRIDEiA6H772.

Interactioni

Subunit structurei

Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits By similarity. Component of the PP4 complexes PPP4C-PPP4R1, PPP4C-PPP4R2, PPP4C-PPP4R2-PPP4R3A, PPP4C-PPP4R2-PPP4R3B and PPP4C-PPP4R4 By similarity. The PPP4C-PPP4R2 complex appears to be a tetramer composed of 2 molecules of PPP4C and 2 molecules of PPP4R2 By similarity. Interacts with REL, NFKB1/p50 and RELA By similarity. Interacts with SMN1 AND GEMIN4. Interacts with IRS4 (phosphorylated) By similarity. Interacts with SMEK1/PPP4R3A; the interaction requires PP4R2 By similarity. Interacts with HDAC3 By similarity.

Binary interactionsi

WithEntry#Exp.IntActNotes
PPP4R1F1MRS13EBI-2638542,EBI-2638538

Protein-protein interaction databases

IntActiA6H772. 1 interaction.
STRINGi9913.ENSBTAP00000017178.

Structurei

3D structure databases

ProteinModelPortaliA6H772.
SMRiA6H772. Positions 6-291.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0639.
GeneTreeiENSGT00550000074618.
HOGENOMiHOG000172696.
HOVERGENiHBG000216.
InParanoidiA6H772.
KOiK15423.
OMAiVFNHRND.
OrthoDBiEOG74N5H2.
TreeFamiTF105559.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A6H772-1 [UniParc]FASTAAdd to Basket

« Hide

MAEISDLDRQ IEQLRRCELI KESEVKALCA KAREILVEES NVQRVDSPVT    50
VCGDIHGQFY DLKELFRVGG DVPETNYLFM GDFVDRGFYS VETFLLLLAL 100
KVRYPDRITL IRGNHESRQI TQVYGFYDEC LRKYGSVTVW RYCTEIFDYL 150
SLSAIIDGKI FCVHGGLSPS IQTLDQIRTI DRKQEVPHDG PMCDLLWSDP 200
EDTTGWGVSP RGAGYLFGSD VVAQFNAAND IDMICRAHQL VMEGYKWHFN 250
ETVLTVWSAP NYCYRCGNVA AILELDEHLQ KDFIIFEAAP QETRGIPSKK 300
PVADYFL 307
Length:307
Mass (Da):35,080
Last modified:July 24, 2007 - v1
Checksum:iD6FE470A5C6CBCAC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC146137 mRNA. Translation: AAI46138.1.
RefSeqiNP_001092578.1. NM_001099108.2.
XP_005225004.1. XM_005224947.1.
XP_005225005.1. XM_005224948.1.
UniGeneiBt.42027.

Genome annotation databases

EnsembliENSBTAT00000017178; ENSBTAP00000017178; ENSBTAG00000012928.
GeneIDi540398.
KEGGibta:540398.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC146137 mRNA. Translation: AAI46138.1 .
RefSeqi NP_001092578.1. NM_001099108.2.
XP_005225004.1. XM_005224947.1.
XP_005225005.1. XM_005224948.1.
UniGenei Bt.42027.

3D structure databases

ProteinModelPortali A6H772.
SMRi A6H772. Positions 6-291.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi A6H772. 1 interaction.
STRINGi 9913.ENSBTAP00000017178.

Proteomic databases

PRIDEi A6H772.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000017178 ; ENSBTAP00000017178 ; ENSBTAG00000012928 .
GeneIDi 540398.
KEGGi bta:540398.

Organism-specific databases

CTDi 5531.

Phylogenomic databases

eggNOGi COG0639.
GeneTreei ENSGT00550000074618.
HOGENOMi HOG000172696.
HOVERGENi HBG000216.
InParanoidi A6H772.
KOi K15423.
OMAi VFNHRND.
OrthoDBi EOG74N5H2.
TreeFami TF105559.

Miscellaneous databases

NextBioi 20878609.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view ]
Pfami PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR00114. STPHPHTASE.
SMARTi SM00156. PP2Ac. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal lung.

Entry informationi

Entry nameiPP4C_BOVIN
AccessioniPrimary (citable) accession number: A6H772
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: July 24, 2007
Last modified: June 11, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi