Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mitochondrial intermediate peptidase

Gene

Mipep

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cleaves proteins, imported into the mitochondrion, to their mature size.By similarity

Catalytic activityi

Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion.By similarity

Enzyme regulationi

Activity is divalent cation-dependent. It is stimulated by manganese, magnesium or calcium ions and reversibly inhibited by zinc, cobalt and iron (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi493Zinc; catalyticPROSITE-ProRule annotation1
Active sitei494PROSITE-ProRule annotation1
Metal bindingi497Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi500Zinc; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Metalloprotease, Protease
LigandCalcium, Cobalt, Iron, Magnesium, Manganese, Metal-binding, Zinc

Protein family/group databases

MEROPSiM03.006

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial intermediate peptidase (EC:3.4.24.59)
Short name:
MIP
Gene namesi
Name:Mipep
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1917728 Mipep

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 33MitochondrionBy similarityAdd BLAST33
ChainiPRO_000031904534 – 711Mitochondrial intermediate peptidaseAdd BLAST678

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei124N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiA6H611
MaxQBiA6H611
PaxDbiA6H611
PeptideAtlasiA6H611
PRIDEiA6H611

PTM databases

iPTMnetiA6H611
PhosphoSitePlusiA6H611

Expressioni

Gene expression databases

BgeeiENSMUSG00000021993
CleanExiMM_MIPEP
GenevisibleiA6H611 MM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000069840

Structurei

3D structure databases

ProteinModelPortaliA6H611
SMRiA6H611
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M3 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2090 Eukaryota
COG0339 LUCA
GeneTreeiENSGT00550000075047
HOGENOMiHOG000230535
HOVERGENiHBG008215
InParanoidiA6H611
KOiK01410
OMAiVENTAWQ
OrthoDBiEOG091G03FL
PhylomeDBiA6H611
TreeFamiTF105715

Family and domain databases

CDDicd06457 M3A_MIP, 1 hit
Gene3Di1.10.1370.10, 2 hits
InterProiView protein in InterPro
IPR033851 M3A_MIP
IPR024077 Neurolysin/TOP_dom2
IPR001567 Pept_M3A_M3B
PANTHERiPTHR11804:SF5 PTHR11804:SF5, 1 hit
PfamiView protein in Pfam
PF01432 Peptidase_M3, 1 hit
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A6H611-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLAAGARYA RRLCGRGAAA ALQGRTGRSC ARDVSTSWSP VGAAFNVKPQ
60 70 80 90 100
SHLWNLLGER RGLFGVPELS TPEGFQVAQE EALKKTEWLV ERACSTPPGP
110 120 130 140 150
QTVLIFDELS DCLCRVADLA DFVKIGHPDP AFREAAQEAC RSIGTMVEKL
160 170 180 190 200
NTNVELYQSL QRLLGDKKLM ESLDAETRRV AELFMFDFEI SGIHLDEEKR
210 220 230 240 250
RRAVDLNVKI LDLSNAFLMR TNFPNKIRKS LLPEHIQHHF ARDGSHLIID
260 270 280 290 300
GLHAEASDDL VREAAYKIFL YPNADQLKCL EELLSSRDLL AKLVGYSTFS
310 320 330 340 350
HRALQGTIAQ TPETVMQFLE KLSEKLSERT RKDFKMMQGM KTKLNPQNSK
360 370 380 390 400
LMPWDPPYYS GVIRAERYNI EPSLYCPFLS LGACMEGLNV LFNKLLGITL
410 420 430 440 450
YAEQTFKGEV WCNDIRKLAV VHESEGLLGY IYCDFFQRAN KPQQDCHFTI
460 470 480 490 500
RGGRLKEDGS YQLPVVVLML NLPHASRDFP TLLTPGMMEN LFHEMGHAMH
510 520 530 540 550
SMLGRTRYQH VTGTRCPTDF AEVPSILMEY FSNDYRVVSQ FAKHYQTGQP
560 570 580 590 600
LPKAMVSRLC ESKKVCTAAE MQLQVFYAAL DQIYHGQHPL KKSTTDILME
610 620 630 640 650
TQEQFYGLPY VPDTAWQLRF SHLVGYGAKY YSYLMSRAVA SMIWKECFLQ
660 670 680 690 700
DPFNRAAGER YRREMLAHGG GKEPMLMIQG MLQKCPSIDD FVDALVSDMN
710
LDFETFFLDS K
Length:711
Mass (Da):80,852
Last modified:July 24, 2007 - v1
Checksum:iC22E9FFFB86A7ECB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61R → RVP in BAE27162 (PubMed:16141072).Curated1
Sequence conflicti286S → N in BAE27162 (PubMed:16141072).Curated1
Sequence conflicti419A → T in BAE27162 (PubMed:16141072).Curated1
Sequence conflicti508Y → H in AAI10359 (PubMed:15489334).Curated1
Sequence conflicti595T → A in BAE27162 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK146428 mRNA Translation: BAE27162.1
BC049871 mRNA Translation: AAH49871.1
BC110358 mRNA Translation: AAI10359.1
BC145711 mRNA Translation: AAI45712.1
CCDSiCCDS49517.1
RefSeqiNP_081712.2, NM_027436.3
UniGeneiMm.274650
Mm.486410

Genome annotation databases

EnsembliENSMUST00000063562; ENSMUSP00000069840; ENSMUSG00000021993
ENSMUST00000224635; ENSMUSP00000153502; ENSMUSG00000021993
GeneIDi70478
KEGGimmu:70478

Similar proteinsi

Entry informationi

Entry nameiMIPEP_MOUSE
AccessioniPrimary (citable) accession number: A6H611
Secondary accession number(s): Q2YD79, Q3UJJ3, Q80VA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: July 24, 2007
Last modified: May 23, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health