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A6H611

- MIPEP_MOUSE

UniProt

A6H611 - MIPEP_MOUSE

Protein

Mitochondrial intermediate peptidase

Gene

Mipep

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 63 (01 Oct 2014)
      Sequence version 1 (24 Jul 2007)
      Previous versions | rss
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    Functioni

    Cleaves proteins, imported into the mitochondrion, to their mature size.By similarity

    Catalytic activityi

    Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.

    Cofactori

    Binds 1 zinc ion.By similarity

    Enzyme regulationi

    Activity is divalent cation-dependent. It is stimulated by manganese, magnesium or calcium ions and reversibly inhibited by zinc, cobalt and iron By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi493 – 4931Zinc; catalyticPROSITE-ProRule annotation
    Active sitei494 – 4941PROSITE-ProRule annotation
    Metal bindingi497 – 4971Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi500 – 5001Zinc; catalyticPROSITE-ProRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. metalloendopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Calcium, Cobalt, Iron, Magnesium, Manganese, Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM03.A05.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitochondrial intermediate peptidase (EC:3.4.24.59)
    Short name:
    MIP
    Gene namesi
    Name:Mipep
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:1917728. Mipep.

    Subcellular locationi

    Mitochondrion matrix By similarity

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell
    2. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3333MitochondrionBy similarityAdd
    BLAST
    Chaini34 – 711678Mitochondrial intermediate peptidasePRO_0000319045Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei124 – 1241N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiA6H611.
    PaxDbiA6H611.
    PRIDEiA6H611.

    PTM databases

    PhosphoSiteiA6H611.

    Expressioni

    Gene expression databases

    BgeeiA6H611.
    CleanExiMM_MIPEP.
    GenevestigatoriA6H611.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliA6H611.
    SMRiA6H611. Positions 97-692.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M3 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0339.
    GeneTreeiENSGT00550000075047.
    HOGENOMiHOG000230535.
    HOVERGENiHBG008215.
    InParanoidiA6H611.
    KOiK01410.
    OMAiNADLMEC.
    OrthoDBiEOG7TTQ72.
    PhylomeDBiA6H611.
    TreeFamiTF105715.

    Family and domain databases

    Gene3Di1.10.1370.10. 2 hits.
    1.20.1050.40. 1 hit.
    3.40.390.10. 1 hit.
    InterProiIPR024079. MetalloPept_cat_dom.
    IPR024077. Neurolysin/TOP_dom2.
    IPR024080. Neurolysin/TOP_N.
    IPR001567. Pept_M3A_M3B.
    [Graphical view]
    PfamiPF01432. Peptidase_M3. 1 hit.
    [Graphical view]
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A6H611-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLAAGARYA RRLCGRGAAA ALQGRTGRSC ARDVSTSWSP VGAAFNVKPQ    50
    SHLWNLLGER RGLFGVPELS TPEGFQVAQE EALKKTEWLV ERACSTPPGP 100
    QTVLIFDELS DCLCRVADLA DFVKIGHPDP AFREAAQEAC RSIGTMVEKL 150
    NTNVELYQSL QRLLGDKKLM ESLDAETRRV AELFMFDFEI SGIHLDEEKR 200
    RRAVDLNVKI LDLSNAFLMR TNFPNKIRKS LLPEHIQHHF ARDGSHLIID 250
    GLHAEASDDL VREAAYKIFL YPNADQLKCL EELLSSRDLL AKLVGYSTFS 300
    HRALQGTIAQ TPETVMQFLE KLSEKLSERT RKDFKMMQGM KTKLNPQNSK 350
    LMPWDPPYYS GVIRAERYNI EPSLYCPFLS LGACMEGLNV LFNKLLGITL 400
    YAEQTFKGEV WCNDIRKLAV VHESEGLLGY IYCDFFQRAN KPQQDCHFTI 450
    RGGRLKEDGS YQLPVVVLML NLPHASRDFP TLLTPGMMEN LFHEMGHAMH 500
    SMLGRTRYQH VTGTRCPTDF AEVPSILMEY FSNDYRVVSQ FAKHYQTGQP 550
    LPKAMVSRLC ESKKVCTAAE MQLQVFYAAL DQIYHGQHPL KKSTTDILME 600
    TQEQFYGLPY VPDTAWQLRF SHLVGYGAKY YSYLMSRAVA SMIWKECFLQ 650
    DPFNRAAGER YRREMLAHGG GKEPMLMIQG MLQKCPSIDD FVDALVSDMN 700
    LDFETFFLDS K 711
    Length:711
    Mass (Da):80,852
    Last modified:July 24, 2007 - v1
    Checksum:iC22E9FFFB86A7ECB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti61 – 611R → RVP in BAE27162. (PubMed:16141072)Curated
    Sequence conflicti286 – 2861S → N in BAE27162. (PubMed:16141072)Curated
    Sequence conflicti419 – 4191A → T in BAE27162. (PubMed:16141072)Curated
    Sequence conflicti508 – 5081Y → H in AAI10359. (PubMed:15489334)Curated
    Sequence conflicti595 – 5951T → A in BAE27162. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK146428 mRNA. Translation: BAE27162.1.
    BC049871 mRNA. Translation: AAH49871.1.
    BC110358 mRNA. Translation: AAI10359.1.
    BC145711 mRNA. Translation: AAI45712.1.
    CCDSiCCDS49517.1.
    RefSeqiNP_081712.2. NM_027436.3.
    UniGeneiMm.274650.
    Mm.486410.

    Genome annotation databases

    EnsembliENSMUST00000063562; ENSMUSP00000069840; ENSMUSG00000021993.
    GeneIDi70478.
    KEGGimmu:70478.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK146428 mRNA. Translation: BAE27162.1 .
    BC049871 mRNA. Translation: AAH49871.1 .
    BC110358 mRNA. Translation: AAI10359.1 .
    BC145711 mRNA. Translation: AAI45712.1 .
    CCDSi CCDS49517.1.
    RefSeqi NP_081712.2. NM_027436.3.
    UniGenei Mm.274650.
    Mm.486410.

    3D structure databases

    ProteinModelPortali A6H611.
    SMRi A6H611. Positions 97-692.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi M03.A05.

    PTM databases

    PhosphoSitei A6H611.

    Proteomic databases

    MaxQBi A6H611.
    PaxDbi A6H611.
    PRIDEi A6H611.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000063562 ; ENSMUSP00000069840 ; ENSMUSG00000021993 .
    GeneIDi 70478.
    KEGGi mmu:70478.

    Organism-specific databases

    CTDi 4285.
    MGIi MGI:1917728. Mipep.

    Phylogenomic databases

    eggNOGi COG0339.
    GeneTreei ENSGT00550000075047.
    HOGENOMi HOG000230535.
    HOVERGENi HBG008215.
    InParanoidi A6H611.
    KOi K01410.
    OMAi NADLMEC.
    OrthoDBi EOG7TTQ72.
    PhylomeDBi A6H611.
    TreeFami TF105715.

    Miscellaneous databases

    NextBioi 331711.
    PROi A6H611.
    SOURCEi Search...

    Gene expression databases

    Bgeei A6H611.
    CleanExi MM_MIPEP.
    Genevestigatori A6H611.

    Family and domain databases

    Gene3Di 1.10.1370.10. 2 hits.
    1.20.1050.40. 1 hit.
    3.40.390.10. 1 hit.
    InterProi IPR024079. MetalloPept_cat_dom.
    IPR024077. Neurolysin/TOP_dom2.
    IPR024080. Neurolysin/TOP_N.
    IPR001567. Pept_M3A_M3B.
    [Graphical view ]
    Pfami PF01432. Peptidase_M3. 1 hit.
    [Graphical view ]
    PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: DBA/2.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II and FVB/N.
      Tissue: Brain and Mammary tumor.

    Entry informationi

    Entry nameiMIPEP_MOUSE
    AccessioniPrimary (citable) accession number: A6H611
    Secondary accession number(s): Q2YD79, Q3UJJ3, Q80VA3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: July 24, 2007
    Last modified: October 1, 2014
    This is version 63 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3