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A6H2H7 (SYE_FLAPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS
Gene names
Name:gltX
Ordered Locus Names:FP2499
OrganismFlavobacterium psychrophilum (strain JIP02/86 / ATCC 49511) [Reference proteome] [HAMAP]
Taxonomic identifier402612 [NCBI]
Taxonomic lineageBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeFlavobacterium

Protein attributes

Sequence length501 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 501501Glutamate--tRNA ligase HAMAP-Rule MF_00022
PRO_0000330971

Regions

Motif11 – 2111"HIGH" region HAMAP-Rule MF_00022
Motif260 – 2645"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2631ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A6H2H7 [UniParc].

Last modified July 24, 2007. Version 1.
Checksum: 34DB2A7CDB03126F

FASTA50157,538
        10         20         30         40         50         60 
MSKQVRVRFA PSPTGALHIG GVRTALFNYL FAKKHNGVFY LRIEDTDQNR FVPGAEKYIL 

        70         80         90        100        110        120 
EALKWLGISP DETIGKNEKF GPYRQSERKH LYKQYADLLI NSGWAYYAFD TSESLDTLRK 

       130        140        150        160        170        180 
ITEAAGNTFI YNHTVRETLD NSLTNSPEKV AERIANGEDY VIRFKTPVNE TLHLKDIIRG 

       190        200        210        220        230        240 
DIKFETNLLD DKVLFKSDGM PTYHLANIVD DHLMETSHVI RGEEWLPSMP LHVLLYRAFG 

       250        260        270        280        290        300 
WEAPEFAHLP LILKPVGNGK LSKRDGEKLG FPVFPLQWKT NEGTSLGYKE NGFFPETVIN 

       310        320        330        340        350        360 
FLAMLGWNDG TQQEIFSLEE LVQKFDLNRI HKAGAKFDPE KNKWFNHQYL VKKEDSTLAE 

       370        380        390        400        410        420 
LFLPILASKG IVTNLDYTTK VVALVKERAH FVNELWDLSD YFFIAPTSYD EKATKNWKEE 

       430        440        450        460        470        480 
TPELMKQLIV VLNGIEDFTS LNIETIVKDW MTQNEIGMGK VMQPFRLSLV GKMMGPHLFD 

       490        500 
IIEMIGKKET INRIEKAIAT L 

« Hide

References

[1]"Complete genome sequence of the fish pathogen Flavobacterium psychrophilum."
Duchaud E., Boussaha M., Loux V., Bernardet J.-F., Michel C., Kerouault B., Mondot S., Nicolas P., Bossy R., Caron C., Bessieres P., Gibrat J.-F., Claverol S., Dumetz F., Le Henaff M., Benmansour A.
Nat. Biotechnol. 25:763-769(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JIP02/86 / ATCC 49511.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM398681 Genomic DNA. Translation: CAL44551.1.
RefSeqYP_001297352.1. NC_009613.3.

3D structure databases

ProteinModelPortalA6H2H7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING402612.FP2499.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL44551; CAL44551; FP2499.
GeneID5299855.
KEGGfps:FP2499.
PATRIC21910050. VBIFlaPsy4505_2558.

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252720.
KOK01885.
OMAWINGYYL.
OrthoDBEOG6DRPF7.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
TIGRFAMsTIGR00464. gltX_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE_FLAPJ
AccessionPrimary (citable) accession number: A6H2H7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: July 24, 2007
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries