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A6H244 (SYL_FLAPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:FP2363
OrganismFlavobacterium psychrophilum (strain JIP02/86 / ATCC 49511) [Reference proteome] [HAMAP]
Taxonomic identifier402612 [NCBI]
Taxonomic lineageBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeFlavobacterium

Protein attributes

Sequence length962 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 962962Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009339

Regions

Motif40 – 5112"HIGH" region HAMAP-Rule MF_00049
Motif737 – 7415"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site7401ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A6H244 [UniParc].

Last modified July 24, 2007. Version 1.
Checksum: 517F77D040A253F0

FASTA962109,354
        10         20         30         40         50         60 
MKYNPNEIDA KWQKNWANNQ TFAASNTSIK PKYYILDMFP YPSGAGLHVG HPLGYIASDI 

        70         80         90        100        110        120 
VARFKRHKGF NVLHPQGYDS FGLPAEQYAI QTGQHPDKTT KENIARYREQ LDKIGFSFDW 

       130        140        150        160        170        180 
SREIRTSNAN YYKHTQWIFI QLFNSWYNKS NDKAEDITTL ISLFQKEGNT NINAVCDDNI 

       190        200        210        220        230        240 
APFSAQDWTG FSSSKQQKIL LQYRLTYLAE TEVNWCPGLG TVLANDEIIN GVSERGGHPV 

       250        260        270        280        290        300 
IRKKMTQWSM RISAYAERLL QGLNDIDWSE SIKESQRNWI GKSVGAMVSF KVKNQKSQVT 

       310        320        330        340        350        360 
SEATLSDLRP STLDYIEVFT TRPDTIFGVT FMTLAPEHPL VQEITTPEQK AAIEAYIEKT 

       370        380        390        400        410        420 
AKRSERERMA DVKTISGVFT GAYAEHPFTK EQIPVWIGDY VLAGYGTGAV MAVPCGDERD 

       430        440        450        460        470        480 
YAFANFFKGQ NGMPEIKNIF DNVDIATEAY GSKDNVIIAN SDFLNGLNYK QATKKAIAEL 

       490        500        510        520        530        540 
ETLNQGKGKT NYRLRDAVFS RQRYWGEPFP VYYVNGLPQM IDAKHLPIAL PEVEKYLPTE 

       550        560        570        580        590        600 
DGLPPLGNSA KWAWNTITNE VDFNENINNT TIYPLELNTM PGWAGSSWYW MRYMDPHNDN 

       610        620        630        640        650        660 
EFASPEAIKY WESVDLYIGG SEHATGHLLY ARFWNKFLKD KGYAPTEEPF KKLINQGMIL 

       670        680        690        700        710        720 
GNSAFVYRSE DSKKLYSKGL ISDKTTQAIH VDLAIINETT NELDIEAFKK HPLYSDYANA 

       730        740        750        760        770        780 
EFILENGKYI VGREVEKMSK SKYNVVNPDD ICNEYGADTL RLYEMFLGPL EQSKPWNTAG 

       790        800        810        820        830        840 
ITGVFGFLKK LWRLYFDDNG LIVTNNEPSK ESLKSLHKTI KKVTEDIENF SFNTSVSQFM 

       850        860        870        880        890        900 
ICVNELGTQN CHERAILEPL AIILSPYAPH IAEELWSQLG NSESISTVAF PICHEKYLVE 

       910        920        930        940        950        960 
SDKEYPVSFN GKMKFTINLP LDLTPAQIEE IIMKDERTIK QLDGNTPKKV IIIPGKVINL 


VG 

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References

[1]"Complete genome sequence of the fish pathogen Flavobacterium psychrophilum."
Duchaud E., Boussaha M., Loux V., Bernardet J.-F., Michel C., Kerouault B., Mondot S., Nicolas P., Bossy R., Caron C., Bessieres P., Gibrat J.-F., Claverol S., Dumetz F., Le Henaff M., Benmansour A.
Nat. Biotechnol. 25:763-769(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JIP02/86 / ATCC 49511.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM398681 Genomic DNA. Translation: CAL44418.1.
RefSeqYP_001297219.1. NC_009613.3.

3D structure databases

ProteinModelPortalA6H244.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING402612.FP2363.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL44418; CAL44418; FP2363.
GeneID5299631.
KEGGfps:FP2363.
PATRIC21909768. VBIFlaPsy4505_2420.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 4 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_FLAPJ
AccessionPrimary (citable) accession number: A6H244
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 24, 2007
Last modified: May 14, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries