A6H115 (A6H115_FLAPJ) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Name:	topA EMBL CAL44039.1
Ordered Locus Names:	FP1975

Organism

Flavobacterium psychrophilum (strain JIP02/86 / ATCC 49511)

Taxonomic identifier

402612 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Flavobacterium

Protein attributes

Sequence length	838 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	A6H115 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: D5A5D83CB40C0536	FASTA	838	94,030
	10 20 30 40 50 60 MAKNLVIVES PAKAKTIEKF LGADFQVESS YGHIADLPSK EIGVDVLNGF TPKYEVSPDK 70 80 90 100 110 120 KALVTKLKGL AKKADMVWLA SDEDREGEAI SWHLAEELKL DPKKTKRIVF HEITKTAILK 130 140 150 160 170 180 AIENPREIDY NLVNAQQARR VLDRLVGYEL SPVLWRKIKG GLSAGRVQSV SVRLIVERER 190 200 210 220 230 240 EIINFKPVAT YSVQAEFTNE AGKSFKAKLP KNFNTKKEAE DFLNKNIGSI YKVGDLETKP 250 260 270 280 290 300 TKKTPAGPFT TSTLQQEAAR KLYLPVGITM QLAQRLYEAG LITYMRTDSV NLSKDAMEAA 310 320 330 340 350 360 QAEIIKSYGK EYAKPRTFVN KSKGAQEAHE AIRPTDMSRH TVNIDRDQAR LYDLIWKRTL 370 380 390 400 410 420 ASQMADAELE RTNVKIEANN HSELFTASGE VIKFEGFLKV YLEGHDDDEE EQEGMLPSMK 430 440 450 460 470 480 VNEKLINNYI TATERYSRAA ARYTEASLVK KLEELGIGRP STYAPTISTI INRNYVEKGN 490 500 510 520 530 540 LEGQERNYTQ LTLQSGKVGE KLLKENTGSD KGKLVPTDIG TIVTDFLVKN FDNILDYNFT 550 560 570 580 590 600 AKVEQDFDEI AEGNMQWAKM MQDFYDKFHP TVKDVEANAD RESGERILGI DPKSGKPVSV 610 620 630 640 650 660 RLGKFGPMAQ IGAADDEEKT FASLLKEQNI GNITLEEVLK LFLLPKNLGT YKGEEIEVSN 670 680 690 700 710 720 GRYGPFIRFG TTFVSLPKGE EPLNVDLNRA QELIDEKLLA DAPIAVYKGE GVQKGTGRFG 730 740 750 760 770 780 PFLKWNGIFI NVSTKYNFNN LSQQDIETII EDKIQKNIDK VIHNWEAEGI LVQKARWGRS 790 800 810 820 830 EITKGKIKIE LSKDFDAAKL TLAEAQEMIA KKTPVKKAPV KKAVAKKPAV KKLVAKKN

References

[1]

"Complete genome sequence of the fish pathogen Flavobacterium psychrophilum." Duchaud E., Boussaha M., Loux V., Bernardet J.-F., Michel C., Kerouault B., Mondot S., Nicolas P., Bossy R., Caron C., Bessieres P., Gibrat J.-F., Claverol S., Dumetz F., Le Henaff M., Benmansour A. Nat. Biotechnol. 25:763-769(2007) [PubMed: 17592475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JIP02/86 / ATCC 49511.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM398681 Genomic DNA. Translation: CAL44039.1.
RefSeq	YP_001296841.1. NC_009613.1.
3D structure databases
ProteinModelPortal	A6H115.
ModBase	Search...
Protein-protein interaction databases
STRING	A6H115.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5299397.
GenomeReviews	Gene locus FP1975 in contig AM398681_GR.
KEGG	fps:FP1975.
NMPDR	fig|402612.4.peg.1908.
PATRIC	21908908. VBIFlaPsy4505_1998.
Phylogenomic databases
eggNOG	COG0550.
HOGENOM	HBG512935.
OMA	EEYWSID.
ProtClustDB	CLSK2533776.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 3 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 2 hits. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A6H115_FLAPJ

Accession

Primary (citable) accession number: A6H115

Entry history

Integrated into UniProtKB/TrEMBL:	July 24, 2007
Last sequence update:	July 24, 2007
Last modified:	December 14, 2011
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)