A6H0E7 (DEF_FLAPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptide deformylase
Short name=PDF
EC=3.5.1.88

Alternative name(s):
Polypeptide deformylase

Gene names

Name:	def
Ordered Locus Names:	FP1753

Organism

Flavobacterium psychrophilum (strain JIP02/86 / ATCC 49511) [Reference proteome] [HAMAP]

Taxonomic identifier

402612 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Bacteroidetes/Chlorobi group › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Flavobacterium › Flavobacterium psychrophilum

Protein attributes

Sequence length	194 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity. HAMAP-Rule MF_00163
Catalytic activity	Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP-Rule MF_00163
Cofactor	Binds 1 Fe2+ ion By similarity.
Sequence similarities	Belongs to the polypeptide deformylase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	Iron Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Molecular_function	iron ion binding Inferred from electronic annotation. Source: InterPro peptide deformylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 194	194	Peptide deformylase HAMAP-Rule MF_00163		PRO_0000301033
Sites
	Active site	148	1	By similarity
	Metal binding	105	1	Iron By similarity
	Metal binding	147	1	Iron By similarity
	Metal binding	151	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6H0E7 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: 21B30F28E66C009F	FASTA	194	22,389
	10 20 30 40 50 60 MILPIVGYGD PVLRKVCEEI TKDYINLEET IANMYETMYN ACGVGLAAPQ VGLPIRLFIV 70 80 90 100 110 120 DADPFSDSDD ISKEEAAALK GFKKTFINAK MLKEEGEEWS FSEGCLSIPD VREDVYRNPN 130 140 150 160 170 180 ITIEYYDENF NKKTEEYNGL IARVIQHEYD HIEGVLFTDK ITVFKKQFIK KKLQNIMEGK 190 ARPDYRMKLV PKKR

References

[1]

"Complete genome sequence of the fish pathogen Flavobacterium psychrophilum." Duchaud E., Boussaha M., Loux V., Bernardet J.-F., Michel C., Kerouault B., Mondot S., Nicolas P., Bossy R., Caron C., Bessieres P., Gibrat J.-F., Claverol S., Dumetz F., Le Henaff M., Benmansour A. Nat. Biotechnol. 25:763-769(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JIP02/86 / ATCC 49511.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM398681 Genomic DNA. Translation: CAL43820.1.
RefSeq	YP_001296627.1. NC_009613.1.
3D structure databases
ProteinModelPortal	A6H0E7.
ModBase	Search...
Protein-protein interaction databases
STRING	402612.FP1753.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAL43820; CAL43820; FP1753.
GeneID	5300112.
KEGG	fps:FP1753.
PATRIC	21908450. VBIFlaPsy4505_1772.
Phylogenomic databases
eggNOG	COG0242.
HOGENOM	HOG000243509.
KO	K01462.
OMA	IQIGVPR.
ProtClustDB	PRK00150.
Family and domain databases
Gene3D	3.90.45.10. 1 hit.
HAMAP	MF_00163. Pep_deformylase.
InterPro	IPR000181. Fmet_deformylase. IPR023635. Peptide_deformylase. [Graphical view]
PANTHER	PTHR10458. PTHR10458. 1 hit.
Pfam	PF01327. Pep_deformylase. 1 hit. [Graphical view]
PIRSF	PIRSF004749. Pep_def. 1 hit.
PRINTS	PR01576. PDEFORMYLASE.
SUPFAM	SSF56420. Fmet_deformylase. 1 hit.
TIGRFAMs	TIGR00079. pept_deformyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DEF_FLAPJ

Accession

Primary (citable) accession number: A6H0E7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	July 24, 2007
Last modified:	May 1, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program