A6H0C4 (KATG_FLAPJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	FP1730

Organism

Flavobacterium psychrophilum (strain JIP02/86 / ATCC 49511)

Taxonomic identifier

402612 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Flavobacterium

Protein attributes

Sequence length	735 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 735	735	Catalase-peroxidase HAMAP MF_01961		PRO_0000354788
Sites
	Active site	104	1	Proton acceptor By similarity
	Metal binding	267	1	Iron (heme axial ligand) By similarity
	Site	100	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	103 ↔ 226		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-252) By similarity
	Cross-link	226 ↔ 252		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-103) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6H0C4 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: 9A0A3B7E7602A885	FASTA	735	81,010
	10 20 30 40 50 60 MENNTNPISG QGKCPFSGGA AKQSAGAGTR NSNWWPNQLK LNILRQYSSL SNPMGEEFNY 70 80 90 100 110 120 AAAFKSLDLI TLKKDISDLM TNSQDWWPAD YGHYGPFFIR MAWHSAGTYR VADGRGGAGF 130 140 150 160 170 180 GMQRFAPLNS WPDNVNLDKA RLLLWPIKQK YGKKISWADL MILAGNCALE SMGFKTFGFA 190 200 210 220 230 240 GGREDVWEPA EDIYWGSEGK WLDDQRYSGD RELENPLAAV QMGLIYVNPE GPNGNPDPLA 250 260 270 280 290 300 SARDIRETFA RMAMNDEETV ALVAGGHTFG KTHGAADPNQ YVSAEPAAAS IEEQGLGWKN 310 320 330 340 350 360 TFGSGSGEHT ISSGLEGAWT TTPAKWSHNY LENLFGFEWE LTKSPAGAHQ WKPKNGAGAG 370 380 390 400 410 420 TVPDAHNSSK SHAPTMLTAD LALRADPIYE KISRRFLENP AEFEEAFARA WFKLTHRDMG 430 440 450 460 470 480 PVARYLGSDV PKEVLIWQDP IPAVNHQLID DNDITILKAK ILDAGFSVSE MVSTAWASAS 490 500 510 520 530 540 TFRGSDKRGG ANGARICLAP QKDWKVNNPI QLSKVLDALK EIQVTFNNAQ SIGKQVSIAD 550 560 570 580 590 600 LIVLTGCVGI EKAAKNIKVP FTPGRTDALQ EQTDVASFAV LEPEADGFRN YMKTQYTVSA 610 620 630 640 650 660 EEMLIDKAQL LTLTAPELTV LIGGLRVLDV NFNQSKNGLF TNRSGELTND FFVNLLDFGT 670 680 690 700 710 720 TWKASSESQD IFEGRNRKTG ELKWTGTRVD LIFGSNSELR ALAEVYGCTD SQEKFVKDFV 730 KAWDKVMNLD RFDLV

References

[1]

"Complete genome sequence of the fish pathogen Flavobacterium psychrophilum." Duchaud E., Boussaha M., Loux V., Bernardet J.-F., Michel C., Kerouault B., Mondot S., Nicolas P., Bossy R., Caron C., Bessieres P., Gibrat J.-F., Claverol S., Dumetz F., Le Henaff M., Benmansour A. Nat. Biotechnol. 25:763-769(2007) [PubMed: 17592475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JIP02/86 / ATCC 49511.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM398681 Genomic DNA. Translation: CAL43797.1.
RefSeq	YP_001296604.1. NC_009613.1.
3D structure databases
ProteinModelPortal	A6H0C4.
SMR	A6H0C4. Positions 28-734.
ModBase	Search...
Protein-protein interaction databases
STRING	A6H0C4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5300104.
GenomeReviews	Gene locus FP1730 in contig AM398681_GR.
KEGG	fps:FP1730.
NMPDR	fig|402612.4.peg.1669.
PATRIC	21908400. VBIFlaPsy4505_1747.
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	KRHAPSM.
ProtClustDB	PRK15061.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_FLAPJ

Accession

Primary (citable) accession number: A6H0C4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	July 24, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program