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A6GZL9 (LIPA_FLAPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipoyl synthase

EC=2.8.1.8
Alternative name(s):
Lip-syn
Short name=LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene names
Name:lipA
Ordered Locus Names:FP1469
OrganismFlavobacterium psychrophilum (strain JIP02/86 / ATCC 49511) [Reference proteome] [HAMAP]
Taxonomic identifier402612 [NCBI]
Taxonomic lineageBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeFlavobacterium

Protein attributes

Sequence length290 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_00206

Catalytic activity

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_00206

Cofactor

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_00206

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00206.

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein lipoylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

lipoate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 290290Lipoyl synthase HAMAP-Rule MF_00206
PRO_1000071728

Sites

Metal binding441Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding491Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding551Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding701Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding741Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding771Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
A6GZL9 [UniParc].

Last modified July 24, 2007. Version 1.
Checksum: B2F2E26EF8F2281B

FASTA29032,926
        10         20         30         40         50         60 
METVLDSNIL PVGKPKWLKV KLPIGQKYTE LRGLVDKYKL NTICTSGSCP NMGECWGEGT 

        70         80         90        100        110        120 
ATFMILGNIC TRSCGFCGVK TGRPETVDWD EPEKVARSIK IMNIKHAVIT SVDRDDLKDM 

       130        140        150        160        170        180 
GSIIWIETVK AIRRMNPETT LETLIPDFQG VERNLDRIVA ANPEVVSHNV ETVRRLTREV 

       190        200        210        220        230        240 
RIQAKYDKSL EVLRYLKAKG IKRTKSGIML GLGETEEEVI QTMRDLREAN VDIVTIGQYL 

       250        260        270        280        290 
QPSKKHLPVK EFITPEQFEK YELLGKEMGF RHVESGPLVR SSYHAQKHIL 

« Hide

References

[1]"Complete genome sequence of the fish pathogen Flavobacterium psychrophilum."
Duchaud E., Boussaha M., Loux V., Bernardet J.-F., Michel C., Kerouault B., Mondot S., Nicolas P., Bossy R., Caron C., Bessieres P., Gibrat J.-F., Claverol S., Dumetz F., Le Henaff M., Benmansour A.
Nat. Biotechnol. 25:763-769(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JIP02/86 / ATCC 49511.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM398681 Genomic DNA. Translation: CAL43542.1.
RefSeqYP_001296351.1. NC_009613.3.

3D structure databases

ProteinModelPortalA6GZL9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING402612.FP1469.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL43542; CAL43542; FP1469.
GeneID5299375.
KEGGfps:FP1469.
PATRIC21907858. VBIFlaPsy4505_1481.

Phylogenomic databases

eggNOGCOG0320.
HOGENOMHOG000235998.
KOK03644.
OMAHPHIPTK.
OrthoDBEOG6038ZS.

Enzyme and pathway databases

UniPathwayUPA00538; UER00593.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00206. Lipoyl_synth.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERPTHR10949. PTHR10949. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF005963. Lipoyl_synth. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00510. lipA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameLIPA_FLAPJ
AccessionPrimary (citable) accession number: A6GZL9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: July 24, 2007
Last modified: May 14, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways