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A6GYX5 (DAPF_FLAPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Diaminopimelate epimerase

Short name=DAP epimerase
EC=5.1.1.7
Gene names
Name:dapF
Ordered Locus Names:FP1215
OrganismFlavobacterium psychrophilum (strain JIP02/86 / ATCC 49511) [Reference proteome] [HAMAP]
Taxonomic identifier402612 [NCBI]
Taxonomic lineageBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeFlavobacterium

Protein attributes

Sequence length256 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan By similarity. HAMAP-Rule MF_00197

Catalytic activity

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate. HAMAP-Rule MF_00197

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1. HAMAP-Rule MF_00197

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00197.

Sequence similarities

Belongs to the diaminopimelate epimerase family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   Molecular functionIsomerase
   PTMDisulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processlysine biosynthetic process via diaminopimelate

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondiaminopimelate epimerase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 256256Diaminopimelate epimerase HAMAP-Rule MF_00197
PRO_1000099237

Regions

Region72 – 743Substrate binding By similarity
Region187 – 1882Substrate binding By similarity
Region198 – 1992Substrate binding By similarity

Sites

Active site721Proton donor/acceptor By similarity
Active site1971Proton donor/acceptor By similarity
Binding site111Substrate By similarity
Binding site451Substrate By similarity
Binding site631Substrate By similarity
Binding site1691Substrate By similarity
Site1371Important for catalytic activity By similarity
Site1871Important for catalytic activity By similarity

Amino acid modifications

Disulfide bond72 ↔ 197 HAMAP-Rule MF_00197

Sequences

Sequence LengthMass (Da)Tools
A6GYX5 [UniParc].

Last modified July 24, 2007. Version 1.
Checksum: 42633776D96E3A8B

FASTA25628,463
        10         20         30         40         50         60 
MKLYKYQGTG NDFIMIDNRL QIFPKQNTAL IQKLCDRRFG IGADGLILLE NDQSTDFKMV 

        70         80         90        100        110        120 
YYNSDGNQST MCGNGGRCLV AFAKKLNIIK NKTTFIAIDG LHHATINEND IISLQMKNVE 

       130        140        150        160        170        180 
EVNIHDNYVF LNTGSPHHVQ FADNLSNFDV KNEGAKIRYS DLYGQAGSNI NFVHQTSPTQ 

       190        200        210        220        230        240 
FSIRTYERGV EDETLSCGTG ATATAIAMKA TGKTNSNNIT INVQGGKLEV SFNQENSIFT 

       250 
NIFLKGPAEF VFETTI 

« Hide

References

[1]"Complete genome sequence of the fish pathogen Flavobacterium psychrophilum."
Duchaud E., Boussaha M., Loux V., Bernardet J.-F., Michel C., Kerouault B., Mondot S., Nicolas P., Bossy R., Caron C., Bessieres P., Gibrat J.-F., Claverol S., Dumetz F., Le Henaff M., Benmansour A.
Nat. Biotechnol. 25:763-769(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JIP02/86 / ATCC 49511.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM398681 Genomic DNA. Translation: CAL43298.1.
RefSeqYP_001296109.1. NC_009613.3.

3D structure databases

ProteinModelPortalA6GYX5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING402612.FP1215.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL43298; CAL43298; FP1215.
GeneID5298992.
KEGGfps:FP1215.
PATRIC21907336. VBIFlaPsy4505_1229.

Phylogenomic databases

eggNOGCOG0253.
HOGENOMHOG000220466.
KOK01778.
OMALIVEPPY.
OrthoDBEOG6ND0M5.

Enzyme and pathway databases

UniPathwayUPA00034; UER00025.

Family and domain databases

HAMAPMF_00197. DAP_epimerase.
InterProIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERPTHR31689. PTHR31689. 1 hit.
PfamPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsTIGR00652. DapF. 1 hit.
PROSITEPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPF_FLAPJ
AccessionPrimary (citable) accession number: A6GYX5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: July 24, 2007
Last modified: June 11, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways