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A6GXD5 (DDL_FLAPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:FP0653
OrganismFlavobacterium psychrophilum (strain JIP02/86 / ATCC 49511) [Reference proteome] [HAMAP]
Taxonomic identifier402612 [NCBI]
Taxonomic lineageBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeFlavobacterium

Protein attributes

Sequence length328 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 328328D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000074775

Regions

Domain118 – 317200ATP-grasp
Nucleotide binding146 – 20156ATP By similarity

Sites

Metal binding2721Magnesium or manganese 1 By similarity
Metal binding2841Magnesium or manganese 1 By similarity
Metal binding2841Magnesium or manganese 2 By similarity
Metal binding2861Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
A6GXD5 [UniParc].

Last modified July 24, 2007. Version 1.
Checksum: 814AD1D737028CB7

FASTA32836,681
        10         20         30         40         50         60 
MNVAVVMGGY SDESVISLRS GQLILNNLDK SKYNIFEVHI LLKDWSVVIE GEKYPINKAD 

        70         80         90        100        110        120 
FSFTKNGVIT KFDVAINTVH GTPGEDGHLQ SYWELIDIPY TGCNFYQSAL TFNKRDTLSV 

       130        140        150        160        170        180 
LTKFNIPKAK SIYLRKGDVI DGNEIKKALG LPFFVKPNQS GSSLGVSKVD ALDQLEKALE 

       190        200        210        220        230        240 
FAFAEDNEIL IESYLNGTEV SVGVLNYKGQ TKVLGITEIL SQNDFFDYEA KYLGKSEEIT 

       250        260        270        280        290        300 
PARISKEEEI LVAESAKKIY NSLGMSGFSR TDFIIMNGIP HFIEINTNPG LSPQSIFPQQ 

       310        320 
AMFAKMDMPQ MLDNEITLAL SRKPIWKK 

« Hide

References

[1]"Complete genome sequence of the fish pathogen Flavobacterium psychrophilum."
Duchaud E., Boussaha M., Loux V., Bernardet J.-F., Michel C., Kerouault B., Mondot S., Nicolas P., Bossy R., Caron C., Bessieres P., Gibrat J.-F., Claverol S., Dumetz F., Le Henaff M., Benmansour A.
Nat. Biotechnol. 25:763-769(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JIP02/86 / ATCC 49511.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM398681 Genomic DNA. Translation: CAL42758.1.
RefSeqYP_001295574.1. NC_009613.3.

3D structure databases

ProteinModelPortalA6GXD5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING402612.FP0653.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL42758; CAL42758; FP0653.
GeneID5300688.
KEGGfps:FP0653.
PATRIC21906180. VBIFlaPsy4505_0671.

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMADTQYRIP.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycFPSY402612:GJEP-1153-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_FLAPJ
AccessionPrimary (citable) accession number: A6GXD5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: July 24, 2007
Last modified: February 19, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways