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A6GWH3 (TYSY_FLAPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:FP0333
OrganismFlavobacterium psychrophilum (strain JIP02/86 / ATCC 49511)
Taxonomic identifier402612 [NCBI]
Taxonomic lineageBacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Flavobacteriaceae › Flavobacterium

Protein attributes

Sequence length274 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008

Subunit structure

Homodimer By similarity. HAMAP MF_00008

Subcellular location

Cytoplasm By similarity HAMAP MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processdTMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionthymidylate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 274274Thymidylate synthase HAMAP MF_00008
PRO_1000000596

Sites

Active site1561 By similarity

Sequences

Sequence LengthMass (Da)Tools
A6GWH3 [UniParc].

Last modified July 24, 2007. Version 1.
Checksum: D61ECAE1E7C09E5D

FASTA27431,299
        10         20         30         40         50         60 
MKQYLDLVKY VLNNGTQKGD RTGTGTKSVF GYQMRFNLAE GFPMVTTKKL HLKSIIHELL 

        70         80         90        100        110        120 
WFLKGETNIA YLQQNGVKIW DDWADENGEL GPVYGHQWRN WNNEEIDQIT ELIETLKINP 

       130        140        150        160        170        180 
NSRRMLVSAW NPSVLPDTTK SFAENVANAK VALPPCHAFF QFYVSEGKLS CQLYQRSADI 

       190        200        210        220        230        240 
FLGVPFNIAS YALFTMMIAQ VCDLQAGEFI HTFGDAHIYN NHFEQVALQL SREPKPLPKM 

       250        260        270 
ILNPAVKNIF DFKFEDFKLE GYESHAHIKG AVAV

« Hide

References

[1]"Complete genome sequence of the fish pathogen Flavobacterium psychrophilum."
Duchaud E., Boussaha M., Loux V., Bernardet J.-F., Michel C., Kerouault B., Mondot S., Nicolas P., Bossy R., Caron C., Bessieres P., Gibrat J.-F., Claverol S., Dumetz F., Le Henaff M., Benmansour A.
Nat. Biotechnol. 25:763-769(2007) [PubMed: 17592475] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JIP02/86 / ATCC 49511.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM398681 Genomic DNA. Translation: CAL42446.1.
RefSeqYP_001295264.1. NC_009613.1.

3D structure databases

ProteinModelPortalA6GWH3.
SMRA6GWH3. Positions 1-274.
ModBaseSearch...

Protein-protein interaction databases

STRINGA6GWH3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5300119.
GenomeReviewsGene locus FP0333 in contig AM398681_GR.
KEGGfps:FP0333.
NMPDRfig|402612.4.peg.328.
PATRIC21905512. VBIFlaPsy4505_0344.

Phylogenomic databases

eggNOGCOG0207.
HOGENOMHBG588098.
OMARMALAPC.
ProtClustDBPRK01827.

Family and domain databases

HAMAPMF_00008. Thymidy_synth_bact.
[Tree]
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
[Graphical view]
Gene3DG3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
KOK00560.
PANTHERPTHR11549:SF2. Thymidylat_synth_C. 1 hit.
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMsTIGR03284. Thym_sym. 2 hits.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_FLAPJ
AccessionPrimary (citable) accession number: A6GWH3
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: July 24, 2007
Last modified: December 14, 2011
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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