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A6GW77

- BIOB_FLAPJ

UniProt

A6GW77 - BIOB_FLAPJ

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Protein

Biotin synthase

Gene
bioB, FP0234
Organism
Flavobacterium psychrophilum (strain JIP02/86 / ATCC 49511)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi58 – 581Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi61 – 611Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi98 – 981Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi130 – 1301Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi190 – 1901Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi262 – 2621Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:FP0234
OrganismiFlavobacterium psychrophilum (strain JIP02/86 / ATCC 49511)
Taxonomic identifieri402612 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeFlavobacterium
ProteomesiUP000006394: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 362362Biotin synthaseUniRule annotationPRO_0000381385Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi402612.FP0234.

Structurei

3D structure databases

ProteinModelPortaliA6GW77.
SMRiA6GW77. Positions 5-316.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A6GW77-1 [UniParc]FASTAAdd to Basket

« Hide

MSVMRNNWTK EEIIAIYNKP LMDLLYEAAS IHREYHDPNV VQVSTLLSIK    50
TGGCPEDCGY CPQAARYHTD IEGNDLMSVQ QVKAQALRAK SSGSSRVCMG 100
AAWRNVKDGP EFDQVLEMVR TINKLDMEVC CTLGMITENQ AHRLAEAGLY 150
AYNHNLDTSE EYYKEVISTR GFEDRIETIA NVRKTNVTVC SGGIIGMGES 200
IGDRAGMLVA LSTLSPQPES VPINALVAVE GTPMEEEKPV EIWEMIRMVA 250
TTRIIMPETQ VRLSAGRMNM TREGQAMCFF AGANSIFAGD KLLTTPNPDV 300
NEDMKMFEML GLNPQKPFTK VSQPTTVEAK DSRYESLGEK PKWSRPSHTI 350
EKNLELSGKG KN 362
Length:362
Mass (Da):40,257
Last modified:July 24, 2007 - v1
Checksum:i4CD0B7A6BC9F9715
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM398681 Genomic DNA. Translation: CAL42350.1.
RefSeqiYP_001295170.1. NC_009613.3.

Genome annotation databases

EnsemblBacteriaiCAL42350; CAL42350; FP0234.
GeneIDi5299299.
KEGGifps:FP0234.
PATRICi21905304. VBIFlaPsy4505_0243.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM398681 Genomic DNA. Translation: CAL42350.1 .
RefSeqi YP_001295170.1. NC_009613.3.

3D structure databases

ProteinModelPortali A6GW77.
SMRi A6GW77. Positions 5-316.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 402612.FP0234.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL42350 ; CAL42350 ; FP0234 .
GeneIDi 5299299.
KEGGi fps:FP0234.
PATRICi 21905304. VBIFlaPsy4505_0243.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JIP02/86 / ATCC 49511.

Entry informationi

Entry nameiBIOB_FLAPJ
AccessioniPrimary (citable) accession number: A6GW77
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 24, 2007
Last modified: May 14, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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