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A6GW77 (BIOB_FLAPJ) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:FP0234
OrganismFlavobacterium psychrophilum (strain JIP02/86 / ATCC 49511) [Reference proteome] [HAMAP]
Taxonomic identifier402612 [NCBI]
Taxonomic lineageBacteriaBacteroidetesFlavobacteriiaFlavobacterialesFlavobacteriaceaeFlavobacterium

Protein attributes

Sequence length362 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 362362Biotin synthase HAMAP-Rule MF_01694
PRO_0000381385

Sites

Metal binding541Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding581Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding611Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding981Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1301Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1901Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2621Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A6GW77 [UniParc].

Last modified July 24, 2007. Version 1.
Checksum: 4CD0B7A6BC9F9715

FASTA36240,257
        10         20         30         40         50         60 
MSVMRNNWTK EEIIAIYNKP LMDLLYEAAS IHREYHDPNV VQVSTLLSIK TGGCPEDCGY 

        70         80         90        100        110        120 
CPQAARYHTD IEGNDLMSVQ QVKAQALRAK SSGSSRVCMG AAWRNVKDGP EFDQVLEMVR 

       130        140        150        160        170        180 
TINKLDMEVC CTLGMITENQ AHRLAEAGLY AYNHNLDTSE EYYKEVISTR GFEDRIETIA 

       190        200        210        220        230        240 
NVRKTNVTVC SGGIIGMGES IGDRAGMLVA LSTLSPQPES VPINALVAVE GTPMEEEKPV 

       250        260        270        280        290        300 
EIWEMIRMVA TTRIIMPETQ VRLSAGRMNM TREGQAMCFF AGANSIFAGD KLLTTPNPDV 

       310        320        330        340        350        360 
NEDMKMFEML GLNPQKPFTK VSQPTTVEAK DSRYESLGEK PKWSRPSHTI EKNLELSGKG 


KN 

« Hide

References

[1]"Complete genome sequence of the fish pathogen Flavobacterium psychrophilum."
Duchaud E., Boussaha M., Loux V., Bernardet J.-F., Michel C., Kerouault B., Mondot S., Nicolas P., Bossy R., Caron C., Bessieres P., Gibrat J.-F., Claverol S., Dumetz F., Le Henaff M., Benmansour A.
Nat. Biotechnol. 25:763-769(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JIP02/86 / ATCC 49511.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM398681 Genomic DNA. Translation: CAL42350.1.
RefSeqYP_001295170.1. NC_009613.3.

3D structure databases

ProteinModelPortalA6GW77.
SMRA6GW77. Positions 5-316.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING402612.FP0234.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL42350; CAL42350; FP0234.
GeneID5299299.
KEGGfps:FP0234.
PATRIC21905304. VBIFlaPsy4505_0243.

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMARIMMPAS.
OrthoDBEOG622PMP.

Enzyme and pathway databases

UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_FLAPJ
AccessionPrimary (citable) accession number: A6GW77
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 24, 2007
Last modified: May 14, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways