Reviewed, UniProtKB/Swiss-Prot A6GW32 (SAHH_FLAPJ)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	FP0189

Organism

Flavobacterium psychrophilum (strain JIP02/86 / ATCC 49511) [Complete proteome] [HAMAP]

Taxonomic identifier

402612 [NCBI]

Taxonomic lineage

Bacteria › Bacteroidetes › Flavobacteria › Flavobacteriales › Flavobacteriaceae › Flavobacterium

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 438	438	Adenosylhomocysteinase HAMAP MF_00563		PRO_1000024726
Regions
	Region	189 – 356	168	NAD binding By similarity
Sites
	Binding site	61	1	Substrate By similarity
	Binding site	137	1	Substrate By similarity
	Binding site	162	1	Substrate By similarity
	Binding site	192	1	Substrate By similarity
	Binding site	196	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A6GW32-1 [UniParc]. Last modified July 24, 2007. Version 1. Checksum: 059C580BF81CED1A	FASTA	438	47,853
	10 20 30 40 50 60 MSTATMPYVA FKVKDISLAA WGRKEIELAE AEMPGLMALR AEYKNEQPLA GARIAGCLHM 70 80 90 100 110 120 TIQTAVLIET LIALGAEVTW SSCNIFSTQD QAAAAIAAAG IQVYAWKGLN EPDFDWCIEQ 130 140 150 160 170 180 TLFFGEDRKP LNMILDDGGD LTNMVIDRYP HLVEGIKGLS EETTTGVHRL YERVKAGTLP 190 200 210 220 230 240 MPAINVNDSV TKSKFDNKYG CKESAVDAVR RATDIMLAGK RVVVCGYGDV GKGTAASFRG 250 260 270 280 290 300 AGSIVTVTEI DPICALQAAM DGFEVKKLNT VVGNADIIIT TTGNKDIVLG SHFEQMKDKT 310 320 330 340 350 360 IVCNIGHFDN EIDMAWLNKN HGASKIEIKP QVDKYTIAGK DILILAEGRL VNLGCATGHP 370 380 390 400 410 420 SFVMSNSFTN QTLAQIELWK NSAAYNNDVY MLPKHLDEKV AFLHLAKLGV ELEVLRDDQA 430 AYIGVGVDGP FKPEYYRY

References

[1]

"Complete genome sequence of the fish pathogen Flavobacterium psychrophilum." Duchaud E., Boussaha M., Loux V., Bernardet J.-F., Michel C., Kerouault B., Mondot S., Nicolas P., Bossy R., Caron C., Bessieres P., Gibrat J.-F., Claverol S., Dumetz F., Le Henaff M., Benmansour A. Nat. Biotechnol. 25:763-769(2007) [PubMed: 17592475] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM398681 Genomic DNA. Translation: CAL42305.1.
RefSeq	YP_001295125.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A6GW32.
Genome annotation databases
GeneID	5298527.
GenomeReviews	Gene locus FP0189 in contig AM398681_GR.
KEGG	fps:FP0189.
NMPDR	fig|402612.4.peg.186.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	HMRAMKD.
Family and domain databases
HAMAP	MF_00563. [Tree]
InterPro	IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR000043. S-Ado-L-homoCys_hydrolase. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_FLAPJ

Accession

Primary (citable) accession number: A6GW32

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	July 24, 2007
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)