Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sialidase-1

Gene

NEU1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage (By similarity).By similarity

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei78SubstrateBy similarity1
Binding sitei97SubstrateBy similarity1
Active sitei103Proton acceptorBy similarity1
Binding sitei264SubstrateBy similarity1
Binding sitei280SubstrateBy similarity1
Binding sitei341SubstrateBy similarity1
Active sitei370NucleophileBy similarity1
Active sitei394Sequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Lipid degradation, Lipid metabolism

Enzyme and pathway databases

ReactomeiR-BTA-1660662. Glycosphingolipid metabolism.
R-BTA-4085001. Sialic acid metabolism.
R-BTA-6798695. Neutrophil degranulation.

Protein family/group databases

CAZyiGH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase-1 (EC:3.2.1.18)
Alternative name(s):
Acetylneuraminyl hydrolase
Lysosomal sialidase
N-acetyl-alpha-neuraminidase 1
Gene namesi
Name:NEU1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 23

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 47By similarityAdd BLAST47
ChainiPRO_000030472648 – 415Sialidase-1Add BLAST368

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi186N-linked (GlcNAc...)Sequence analysis1
Glycosylationi343N-linked (GlcNAc...)Sequence analysis1
Glycosylationi352N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.By similarity
Phosphorylation of tyrosine within the internalization signal results in inhibition of sialidase internalization and blockage on the plasma membrane.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiA6BMK7.
PRIDEiA6BMK7.

Expressioni

Gene expression databases

BgeeiENSBTAG00000005674.

Interactioni

Subunit structurei

Interacts with cathepsin A (protective protein), beta-galactosidase and N-acetylgalactosamine-6-sulfate sulfatase in a multienzyme complex.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007454.

Structurei

3D structure databases

ProteinModelPortaliA6BMK7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati112 – 123BNR 1Add BLAST12
Repeati172 – 183BNR 2Add BLAST12
Repeati231 – 242BNR 3Add BLAST12
Repeati347 – 358BNR 4Add BLAST12

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi77 – 80FRIP motif4
Motifi412 – 415Internalization signal4

Domaini

A C-terminal internalization signal (YGTL) appears to allow the targeting of plasma membrane proteins to endosomes.By similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 33 family.Curated
Contains 4 BNR repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IE33. Eukaryota.
COG4409. LUCA.
GeneTreeiENSGT00720000108878.
HOGENOMiHOG000007651.
HOVERGENiHBG057314.
InParanoidiA6BMK7.
KOiK01186.
OMAiRNQYTES.
OrthoDBiEOG091G0HAW.
TreeFamiTF331063.

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PfamiPF13088. BNR_2. 1 hit.
[Graphical view]
SUPFAMiSSF50939. SSF50939. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A6BMK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEEGPGIVS LGKLRRPRML RLWGICRVQI FSAIFMLMSP AGVGAGAKDD
60 70 80 90 100
FSLVHPLVTM EQLLWVSGKQ IGSVDTFRIP LITTTPRGTL LAFAEARKMS
110 120 130 140 150
TSDKGAKFIA LRRSMDQGST WSPTAFIVDD GETPDGLNLG AVVSDTTTGV
160 170 180 190 200
VFLFYSLCAH KAGCQVASTM LVWSKDDGVS WSSPRNLSLD IGTEMFAPGP
210 220 230 240 250
GSGIQKQREP RKGRLIVCGH GTLERDGVFC LLSDDHGVSW RYGGGVSGIP
260 270 280 290 300
YGQPKRENDF NPDECQPYEL PDGSVVINAR NQNNYHCHCR IILRSYDACD
310 320 330 340 350
TLRPRDVTFD TELVDPVVAA GAVATSSGIV FFSNPAHPEF RVNLTLRWSF
360 370 380 390 400
SNGTSWRKET VQLWPGPSGY SSLTTLEGNV DGKDEAPQLY VLYEKGRNQY
410
MESISLVKVS VYGTL
Length:415
Mass (Da):45,433
Last modified:September 11, 2007 - v2
Checksum:iF670909AF7046428
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti410S → G in BAF64705 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB327106 Genomic DNA. Translation: BAF64705.1.
BC134571 mRNA. Translation: AAI34572.1.
AY937242 Genomic DNA. Translation: AAX14675.1.
RefSeqiNP_001077111.1. NM_001083642.1.
UniGeneiBt.12399.

Genome annotation databases

EnsembliENSBTAT00000007454; ENSBTAP00000007454; ENSBTAG00000005674.
GeneIDi505554.
KEGGibta:505554.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB327106 Genomic DNA. Translation: BAF64705.1.
BC134571 mRNA. Translation: AAI34572.1.
AY937242 Genomic DNA. Translation: AAX14675.1.
RefSeqiNP_001077111.1. NM_001083642.1.
UniGeneiBt.12399.

3D structure databases

ProteinModelPortaliA6BMK7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000007454.

Protein family/group databases

CAZyiGH33. Glycoside Hydrolase Family 33.

Proteomic databases

PaxDbiA6BMK7.
PRIDEiA6BMK7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000007454; ENSBTAP00000007454; ENSBTAG00000005674.
GeneIDi505554.
KEGGibta:505554.

Organism-specific databases

CTDi4758.

Phylogenomic databases

eggNOGiENOG410IE33. Eukaryota.
COG4409. LUCA.
GeneTreeiENSGT00720000108878.
HOGENOMiHOG000007651.
HOVERGENiHBG057314.
InParanoidiA6BMK7.
KOiK01186.
OMAiRNQYTES.
OrthoDBiEOG091G0HAW.
TreeFamiTF331063.

Enzyme and pathway databases

ReactomeiR-BTA-1660662. Glycosphingolipid metabolism.
R-BTA-4085001. Sialic acid metabolism.
R-BTA-6798695. Neutrophil degranulation.

Gene expression databases

BgeeiENSBTAG00000005674.

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PfamiPF13088. BNR_2. 1 hit.
[Graphical view]
SUPFAMiSSF50939. SSF50939. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNEUR1_BOVIN
AccessioniPrimary (citable) accession number: A6BMK7
Secondary accession number(s): A4IFG6, Q5D0G1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: November 30, 2016
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.