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A6BMK7

- NEUR1_BOVIN

UniProt

A6BMK7 - NEUR1_BOVIN

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Protein

Sialidase-1

Gene

NEU1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage (By similarity).By similarity

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei78 – 781SubstrateBy similarity
Binding sitei97 – 971SubstrateBy similarity
Active sitei103 – 1031Proton acceptorBy similarity
Binding sitei264 – 2641SubstrateBy similarity
Binding sitei280 – 2801SubstrateBy similarity
Binding sitei341 – 3411SubstrateBy similarity
Active sitei370 – 3701NucleophileBy similarity
Active sitei394 – 3941Sequence Analysis

GO - Molecular functioni

  1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
  2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
  3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC
  4. exo-alpha-sialidase activity Source: UniProtKB

GO - Biological processi

  1. lipid catabolic process Source: UniProtKB-KW
  2. oligosaccharide catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Lipid degradation, Lipid metabolism

Enzyme and pathway databases

ReactomeiREACT_204575. Glycosphingolipid metabolism.
REACT_213420. Sialic acid metabolism.

Protein family/group databases

CAZyiGH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase-1 (EC:3.2.1.18)
Alternative name(s):
Acetylneuraminyl hydrolase
Lysosomal sialidase
N-acetyl-alpha-neuraminidase 1
Gene namesi
Name:NEU1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 23

Subcellular locationi

Lysosome membrane By similarity; Peripheral membrane protein By similarity; Lumenal side By similarity. Lysosome lumen By similarity. Cell membrane By similarity. Cytoplasmic vesicle By similarity
Note: Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles.By similarity

GO - Cellular componenti

  1. cell junction Source: Ensembl
  2. cytoplasmic vesicle Source: UniProtKB-KW
  3. extracellular vesicular exosome Source: Ensembl
  4. lysosome Source: UniProtKB
  5. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4747By similarityAdd
BLAST
Chaini48 – 415368Sialidase-1PRO_0000304726Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi186 – 1861N-linked (GlcNAc...)Sequence Analysis
Glycosylationi343 – 3431N-linked (GlcNAc...)Sequence Analysis
Glycosylationi352 – 3521N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N-glycosylated.By similarity
Phosphorylation of tyrosine within the internalization signal results in inhibition of sialidase internalization and blockage on the plasma membrane.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiA6BMK7.

Interactioni

Subunit structurei

Interacts with cathepsin A (protective protein), beta-galactosidase and N-acetylgalactosamine-6-sulfate sulfatase in a multienzyme complex.By similarity

Structurei

3D structure databases

ProteinModelPortaliA6BMK7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati112 – 12312BNR 1Add
BLAST
Repeati172 – 18312BNR 2Add
BLAST
Repeati231 – 24212BNR 3Add
BLAST
Repeati347 – 35812BNR 4Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi77 – 804FRIP motif
Motifi412 – 4154Internalization signal

Domaini

A C-terminal internalization signal (YGTL) appears to allow the targeting of plasma membrane proteins to endosomes.By similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 33 family.Curated
Contains 4 BNR repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG4409.
GeneTreeiENSGT00720000108878.
HOGENOMiHOG000007651.
HOVERGENiHBG057314.
InParanoidiA6BMK7.
KOiK01186.
OMAiGTEMFAP.
OrthoDBiEOG779NXT.
TreeFamiTF331063.

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
SUPFAMiSSF50939. SSF50939. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A6BMK7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTEEGPGIVS LGKLRRPRML RLWGICRVQI FSAIFMLMSP AGVGAGAKDD
60 70 80 90 100
FSLVHPLVTM EQLLWVSGKQ IGSVDTFRIP LITTTPRGTL LAFAEARKMS
110 120 130 140 150
TSDKGAKFIA LRRSMDQGST WSPTAFIVDD GETPDGLNLG AVVSDTTTGV
160 170 180 190 200
VFLFYSLCAH KAGCQVASTM LVWSKDDGVS WSSPRNLSLD IGTEMFAPGP
210 220 230 240 250
GSGIQKQREP RKGRLIVCGH GTLERDGVFC LLSDDHGVSW RYGGGVSGIP
260 270 280 290 300
YGQPKRENDF NPDECQPYEL PDGSVVINAR NQNNYHCHCR IILRSYDACD
310 320 330 340 350
TLRPRDVTFD TELVDPVVAA GAVATSSGIV FFSNPAHPEF RVNLTLRWSF
360 370 380 390 400
SNGTSWRKET VQLWPGPSGY SSLTTLEGNV DGKDEAPQLY VLYEKGRNQY
410
MESISLVKVS VYGTL
Length:415
Mass (Da):45,433
Last modified:September 11, 2007 - v2
Checksum:iF670909AF7046428
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti410 – 4101S → G in BAF64705. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB327106 Genomic DNA. Translation: BAF64705.1.
BC134571 mRNA. Translation: AAI34572.1.
AY937242 Genomic DNA. Translation: AAX14675.1.
RefSeqiNP_001077111.1. NM_001083642.1.
UniGeneiBt.12399.

Genome annotation databases

EnsembliENSBTAT00000007454; ENSBTAP00000007454; ENSBTAG00000005674.
GeneIDi505554.
KEGGibta:505554.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB327106 Genomic DNA. Translation: BAF64705.1 .
BC134571 mRNA. Translation: AAI34572.1 .
AY937242 Genomic DNA. Translation: AAX14675.1 .
RefSeqi NP_001077111.1. NM_001083642.1.
UniGenei Bt.12399.

3D structure databases

ProteinModelPortali A6BMK7.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH33. Glycoside Hydrolase Family 33.

Proteomic databases

PRIDEi A6BMK7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSBTAT00000007454 ; ENSBTAP00000007454 ; ENSBTAG00000005674 .
GeneIDi 505554.
KEGGi bta:505554.

Organism-specific databases

CTDi 4758.

Phylogenomic databases

eggNOGi COG4409.
GeneTreei ENSGT00720000108878.
HOGENOMi HOG000007651.
HOVERGENi HBG057314.
InParanoidi A6BMK7.
KOi K01186.
OMAi GTEMFAP.
OrthoDBi EOG779NXT.
TreeFami TF331063.

Enzyme and pathway databases

Reactomei REACT_204575. Glycosphingolipid metabolism.
REACT_213420. Sialic acid metabolism.

Miscellaneous databases

NextBioi 20867196.

Family and domain databases

Gene3Di 2.120.10.10. 1 hit.
InterProi IPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view ]
PANTHERi PTHR10628. PTHR10628. 1 hit.
SUPFAMi SSF50939. SSF50939. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Evaluation of PPGB and NEU1 genes as candidates for the lysosomal storage disorder in Japanese black cattle."
    Masoudi A.A., Yamato O., Kunieda T.
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. NIH - Mammalian Gene Collection (MGC) project
    Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Basal ganglia.
  3. "Estimating probability of parentage in U.S. beef and dairy cattle with single nucleotide polymorphisms."
    Heaton M.P., Clawson M.L., Snelling W.M., Keele J.W., Harhay G.P., Wiedmann R.T., Bennett G.L., Smith T.P.L., Stone R.T., Freking B.A., Van Tassell C.P., Sonstegard T.S., Gasbarre L.C., Carr J., DiBello P., Kumar M., Lee M.S., Murthy J.
    , Otto J., Salisbury B., Schulz V., Tracy R., Hawk D.A., Kalbfleisch T., Laegreid W.W.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 54-205.

Entry informationi

Entry nameiNEUR1_BOVIN
AccessioniPrimary (citable) accession number: A6BMK7
Secondary accession number(s): A4IFG6, Q5D0G1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: October 29, 2014
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3