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A6BMK7

- NEUR1_BOVIN

UniProt

A6BMK7 - NEUR1_BOVIN

Protein

Sialidase-1

Gene

NEU1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 2 (11 Sep 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage By similarity.By similarity

    Catalytic activityi

    Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei78 – 781SubstrateBy similarity
    Binding sitei97 – 971SubstrateBy similarity
    Active sitei103 – 1031Proton acceptorBy similarity
    Binding sitei264 – 2641SubstrateBy similarity
    Binding sitei280 – 2801SubstrateBy similarity
    Binding sitei341 – 3411SubstrateBy similarity
    Active sitei370 – 3701NucleophileBy similarity
    Active sitei394 – 3941Sequence Analysis

    GO - Molecular functioni

    1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
    2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
    3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC
    4. exo-alpha-sialidase activity Source: UniProtKB

    GO - Biological processi

    1. lipid catabolic process Source: UniProtKB-KW
    2. oligosaccharide catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Carbohydrate metabolism, Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    ReactomeiREACT_204575. Glycosphingolipid metabolism.
    REACT_213420. Sialic acid metabolism.

    Protein family/group databases

    CAZyiGH33. Glycoside Hydrolase Family 33.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sialidase-1 (EC:3.2.1.18)
    Alternative name(s):
    Acetylneuraminyl hydrolase
    Lysosomal sialidase
    N-acetyl-alpha-neuraminidase 1
    Gene namesi
    Name:NEU1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 23

    Subcellular locationi

    Lysosome membrane By similarity; Peripheral membrane protein By similarity; Lumenal side By similarity. Lysosome lumen By similarity. Cell membrane By similarity. Cytoplasmic vesicle By similarity
    Note: Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles.By similarity

    GO - Cellular componenti

    1. cell junction Source: Ensembl
    2. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
    3. lysosomal lumen Source: UniProtKB-SubCell
    4. lysosomal membrane Source: UniProtKB-SubCell
    5. lysosome Source: UniProtKB
    6. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasmic vesicle, Lysosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 4747By similarityAdd
    BLAST
    Chaini48 – 415368Sialidase-1PRO_0000304726Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi186 – 1861N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi343 – 3431N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi352 – 3521N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated.By similarity
    Phosphorylation of tyrosine within the internalization signal results in inhibition of sialidase internalization and blockage on the plasma membrane.By similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiA6BMK7.

    Interactioni

    Subunit structurei

    Interacts with cathepsin A (protective protein), beta-galactosidase and N-acetylgalactosamine-6-sulfate sulfatase in a multienzyme complex.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliA6BMK7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati112 – 12312BNR 1Add
    BLAST
    Repeati172 – 18312BNR 2Add
    BLAST
    Repeati231 – 24212BNR 3Add
    BLAST
    Repeati347 – 35812BNR 4Add
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi77 – 804FRIP motif
    Motifi412 – 4154Internalization signal

    Domaini

    A C-terminal internalization signal (YGTL) appears to allow the targeting of plasma membrane proteins to endosomes.By similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 33 family.Curated
    Contains 4 BNR repeats.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiCOG4409.
    GeneTreeiENSGT00720000108878.
    HOGENOMiHOG000007651.
    HOVERGENiHBG057314.
    InParanoidiA6BMK7.
    KOiK01186.
    OMAiGTEMFAP.
    OrthoDBiEOG779NXT.
    TreeFamiTF331063.

    Family and domain databases

    Gene3Di2.120.10.10. 1 hit.
    InterProiIPR026856. Sialidase_fam.
    IPR011040. Sialidases.
    [Graphical view]
    PANTHERiPTHR10628. PTHR10628. 1 hit.
    SUPFAMiSSF50939. SSF50939. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A6BMK7-1 [UniParc]FASTAAdd to Basket

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    MTEEGPGIVS LGKLRRPRML RLWGICRVQI FSAIFMLMSP AGVGAGAKDD    50
    FSLVHPLVTM EQLLWVSGKQ IGSVDTFRIP LITTTPRGTL LAFAEARKMS 100
    TSDKGAKFIA LRRSMDQGST WSPTAFIVDD GETPDGLNLG AVVSDTTTGV 150
    VFLFYSLCAH KAGCQVASTM LVWSKDDGVS WSSPRNLSLD IGTEMFAPGP 200
    GSGIQKQREP RKGRLIVCGH GTLERDGVFC LLSDDHGVSW RYGGGVSGIP 250
    YGQPKRENDF NPDECQPYEL PDGSVVINAR NQNNYHCHCR IILRSYDACD 300
    TLRPRDVTFD TELVDPVVAA GAVATSSGIV FFSNPAHPEF RVNLTLRWSF 350
    SNGTSWRKET VQLWPGPSGY SSLTTLEGNV DGKDEAPQLY VLYEKGRNQY 400
    MESISLVKVS VYGTL 415
    Length:415
    Mass (Da):45,433
    Last modified:September 11, 2007 - v2
    Checksum:iF670909AF7046428
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti410 – 4101S → G in BAF64705. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB327106 Genomic DNA. Translation: BAF64705.1.
    BC134571 mRNA. Translation: AAI34572.1.
    AY937242 Genomic DNA. Translation: AAX14675.1.
    RefSeqiNP_001077111.1. NM_001083642.1.
    UniGeneiBt.12399.

    Genome annotation databases

    EnsembliENSBTAT00000007454; ENSBTAP00000007454; ENSBTAG00000005674.
    GeneIDi505554.
    KEGGibta:505554.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB327106 Genomic DNA. Translation: BAF64705.1 .
    BC134571 mRNA. Translation: AAI34572.1 .
    AY937242 Genomic DNA. Translation: AAX14675.1 .
    RefSeqi NP_001077111.1. NM_001083642.1.
    UniGenei Bt.12399.

    3D structure databases

    ProteinModelPortali A6BMK7.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH33. Glycoside Hydrolase Family 33.

    Proteomic databases

    PRIDEi A6BMK7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000007454 ; ENSBTAP00000007454 ; ENSBTAG00000005674 .
    GeneIDi 505554.
    KEGGi bta:505554.

    Organism-specific databases

    CTDi 4758.

    Phylogenomic databases

    eggNOGi COG4409.
    GeneTreei ENSGT00720000108878.
    HOGENOMi HOG000007651.
    HOVERGENi HBG057314.
    InParanoidi A6BMK7.
    KOi K01186.
    OMAi GTEMFAP.
    OrthoDBi EOG779NXT.
    TreeFami TF331063.

    Enzyme and pathway databases

    Reactomei REACT_204575. Glycosphingolipid metabolism.
    REACT_213420. Sialic acid metabolism.

    Miscellaneous databases

    NextBioi 20867196.

    Family and domain databases

    Gene3Di 2.120.10.10. 1 hit.
    InterProi IPR026856. Sialidase_fam.
    IPR011040. Sialidases.
    [Graphical view ]
    PANTHERi PTHR10628. PTHR10628. 1 hit.
    SUPFAMi SSF50939. SSF50939. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Evaluation of PPGB and NEU1 genes as candidates for the lysosomal storage disorder in Japanese black cattle."
      Masoudi A.A., Yamato O., Kunieda T.
      Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Basal ganglia.
    3. "Estimating probability of parentage in U.S. beef and dairy cattle with single nucleotide polymorphisms."
      Heaton M.P., Clawson M.L., Snelling W.M., Keele J.W., Harhay G.P., Wiedmann R.T., Bennett G.L., Smith T.P.L., Stone R.T., Freking B.A., Van Tassell C.P., Sonstegard T.S., Gasbarre L.C., Carr J., DiBello P., Kumar M., Lee M.S., Murthy J.
      , Otto J., Salisbury B., Schulz V., Tracy R., Hawk D.A., Kalbfleisch T., Laegreid W.W.
      Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 54-205.

    Entry informationi

    Entry nameiNEUR1_BOVIN
    AccessioniPrimary (citable) accession number: A6BMK7
    Secondary accession number(s): A4IFG6, Q5D0G1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: September 11, 2007
    Last modified: October 1, 2014
    This is version 58 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3