Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A6BMK7 (NEUR1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sialidase-1

EC=3.2.1.18
Alternative name(s):
Acetylneuraminyl hydrolase
Lysosomal sialidase
N-acetyl-alpha-neuraminidase 1
Gene names
Name:NEU1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length415 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins and glycolipids. To be active, it is strictly dependent on its presence in the multienzyme complex. Appears to have a preference for alpha 2-3 and alpha 2-6 sialyl linkage By similarity.

Catalytic activity

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Subunit structure

Interacts with cathepsin A (protective protein), beta-galactosidase and N-acetylgalactosamine-6-sulfate sulfatase in a multienzyme complex By similarity.

Subcellular location

Lysosome membrane; Peripheral membrane protein; Lumenal side By similarity. Lysosome lumen By similarity. Cell membrane By similarity. Cytoplasmic vesicle By similarity. Note: Localized not only on the inner side of the lysosomal membrane and in the lysosomal lumen, but also on the plasma membrane and in intracellular vesicles By similarity.

Domain

A C-terminal internalization signal (YGTL) appears to allow the targeting of plasma membrane proteins to endosomes By similarity.

Post-translational modification

N-glycosylated By similarity.

Phosphorylation of tyrosine within the internalization signal results in inhibition of sialidase internalization and blockage on the plasma membrane By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 33 family.

Contains 4 BNR repeats.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4747 By similarity
Chain48 – 415368Sialidase-1
PRO_0000304726

Regions

Repeat112 – 12312BNR 1
Repeat172 – 18312BNR 2
Repeat231 – 24212BNR 3
Repeat347 – 35812BNR 4
Motif77 – 804FRIP motif
Motif412 – 4154Internalization signal

Sites

Active site1031Proton acceptor By similarity
Active site3701Nucleophile By similarity
Active site3941 Potential
Binding site781Substrate By similarity
Binding site971Substrate By similarity
Binding site2641Substrate By similarity
Binding site2801Substrate By similarity
Binding site3411Substrate By similarity

Amino acid modifications

Glycosylation1861N-linked (GlcNAc...) Potential
Glycosylation3431N-linked (GlcNAc...) Potential
Glycosylation3521N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict4101S → G in BAF64705. Ref.1

Sequences

Sequence LengthMass (Da)Tools
A6BMK7 [UniParc].

Last modified September 11, 2007. Version 2.
Checksum: F670909AF7046428

FASTA41545,433
        10         20         30         40         50         60 
MTEEGPGIVS LGKLRRPRML RLWGICRVQI FSAIFMLMSP AGVGAGAKDD FSLVHPLVTM 

        70         80         90        100        110        120 
EQLLWVSGKQ IGSVDTFRIP LITTTPRGTL LAFAEARKMS TSDKGAKFIA LRRSMDQGST 

       130        140        150        160        170        180 
WSPTAFIVDD GETPDGLNLG AVVSDTTTGV VFLFYSLCAH KAGCQVASTM LVWSKDDGVS 

       190        200        210        220        230        240 
WSSPRNLSLD IGTEMFAPGP GSGIQKQREP RKGRLIVCGH GTLERDGVFC LLSDDHGVSW 

       250        260        270        280        290        300 
RYGGGVSGIP YGQPKRENDF NPDECQPYEL PDGSVVINAR NQNNYHCHCR IILRSYDACD 

       310        320        330        340        350        360 
TLRPRDVTFD TELVDPVVAA GAVATSSGIV FFSNPAHPEF RVNLTLRWSF SNGTSWRKET 

       370        380        390        400        410 
VQLWPGPSGY SSLTTLEGNV DGKDEAPQLY VLYEKGRNQY MESISLVKVS VYGTL 

« Hide

References

« Hide 'large scale' references
[1]"Evaluation of PPGB and NEU1 genes as candidates for the lysosomal storage disorder in Japanese black cattle."
Masoudi A.A., Yamato O., Kunieda T.
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Basal ganglia.
[3]"Estimating probability of parentage in U.S. beef and dairy cattle with single nucleotide polymorphisms."
Heaton M.P., Clawson M.L., Snelling W.M., Keele J.W., Harhay G.P., Wiedmann R.T., Bennett G.L., Smith T.P.L., Stone R.T., Freking B.A., Van Tassell C.P., Sonstegard T.S., Gasbarre L.C., Carr J., DiBello P., Kumar M., Lee M.S., Murthy J. expand/collapse author list , Otto J., Salisbury B., Schulz V., Tracy R., Hawk D.A., Kalbfleisch T., Laegreid W.W.
Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 54-205.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB327106 Genomic DNA. Translation: BAF64705.1.
BC134571 mRNA. Translation: AAI34572.1.
AY937242 Genomic DNA. Translation: AAX14675.1.
RefSeqNP_001077111.1. NM_001083642.1.
UniGeneBt.12399.

3D structure databases

ProteinModelPortalA6BMK7.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH33. Glycoside Hydrolase Family 33.

Proteomic databases

PRIDEA6BMK7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000007454; ENSBTAP00000007454; ENSBTAG00000005674.
GeneID505554.
KEGGbta:505554.

Organism-specific databases

CTD4758.

Phylogenomic databases

eggNOGCOG4409.
GeneTreeENSGT00720000108878.
HOGENOMHOG000007651.
HOVERGENHBG057314.
InParanoidA6BMK7.
KOK01186.
OMAGTEMFAP.
OrthoDBEOG779NXT.
TreeFamTF331063.

Family and domain databases

Gene3D2.120.10.10. 1 hit.
InterProIPR026942. Sialidase-1.
IPR026856. Sialidase_fam.
IPR011040. Sialidases.
[Graphical view]
PANTHERPTHR10628. PTHR10628. 1 hit.
PTHR10628:SF9. PTHR10628:SF9. 1 hit.
SUPFAMSSF50939. SSF50939. 1 hit.
ProtoNetSearch...

Other

NextBio20867196.

Entry information

Entry nameNEUR1_BOVIN
AccessionPrimary (citable) accession number: A6BMK7
Secondary accession number(s): A4IFG6, Q5D0G1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: February 19, 2014
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries