Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A5ZY13

- A5ZY13_9FIRM

UniProt

A5ZY13 - A5ZY13_9FIRM

Protein
Submitted name:

Glycosyl hydrolase, family 31

Gene

RUMOBE_03919

Organism
Ruminococcus obeum ATCC 29174
Status
Unreviewed - Annotation score: 1 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 32 (01 Oct 2014)
      Sequence version 1 (24 Jul 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei197 – 1971GlucoseImported
    Binding sitei404 – 4041GlucoseImported
    Binding sitei420 – 4201GlucoseImported
    Binding sitei478 – 4781GlucoseImported

    GO - Molecular functioni

    1. carbohydrate binding Source: InterPro
    2. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    GlycosidaseUniRule annotationImported, Hydrolase

    Protein family/group databases

    CAZyiGH31. Glycoside Hydrolase Family 31.

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Glycosyl hydrolase, family 31Imported (EC:3.2.1.-Imported)
    Gene namesi
    ORF Names:RUMOBE_03919Imported
    OrganismiRuminococcus obeum ATCC 29174Imported
    Taxonomic identifieri411459 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesLachnospiraceaeBlautia

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3M46X-ray2.66A/B1-663[»]
    3M6DX-ray2.90A/B1-663[»]
    3MKKX-ray1.91A/B1-663[»]
    3N04X-ray2.02A/B1-663[»]
    3NSXX-ray1.57A/B1-663[»]
    3NUKX-ray2.06A/B1-663[»]
    3PHAX-ray2.17A/B/C/D1-663[»]
    3POCX-ray1.99A/B1-663[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiA5ZY13.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 31 family.UniRule annotation

    Family and domain databases

    InterProiIPR011013. Gal_mutarotase_SF_dom.
    IPR000322. Glyco_hydro_31.
    IPR025887. Glyco_hydro_31_N_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF13802. Gal_mutarotas_2. 1 hit.
    PF01055. Glyco_hydro_31. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 2 hits.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5ZY13-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIRKYRYGAP FDTEALTEKI ETAEEAFPYG EISQKEGFAF TYIMDEDDIV    50
    YGLGESNRGI NKRGYCYISN CTDDPIHTED KRSLYGAHNF IIVSGKTTFG 100
    LFFDYPSKLT FDIGYTRMDT LKVSCENADL DIYVIEGENA YDIVKQFRRV 150
    IGRSYIPPKF AFGFGQSRWG YTTKEDFRAV AKGYRENHIP IDMIYMDIDY 200
    MQDFKDFTVN EKNFPDFPEF VKEMKDQELR LIPIIDAGVK VEKGYEVYEE 250
    GVKNNYFCKR EDGSDFVAAV WPGDTHFPDM LNPEARKWFG DKYRFLIDQG 300
    IEGFWNDMNE PAIFYSSEGL AEAKEFAGEF AKDTEGKIHP WAMQAKMKDI 350
    VNSPEDYKRF YHNVNGKKIR HDKVHNLFGY NMTRAAGEAF ERIDPEKRFL 400
    MFSRSSYIGM HRYGGIWMGD NKSWWSHILL NLKMLPSLNM CGFMYTGADL 450
    GGFGDDTTRD LLLRFLALGV FTPLMRDHAA EGTREQECYQ FENIEDFRSV 500
    INARYRLVPY LYSEYMKAAL NDDMYFKPLG FVYPDDKMAI RVEDQLMLGN 550
    EIMIAPVYEQ NARGRYVYLP EEMKFIKFMP DGSISEEVLE KGVHYVDVAL 600
    NEVPLFIRSG KCIPVAEAAE CVKDIDTENM QLIGYEGSSY TLYEDDGIHK 650
    DYDKKENYRV LTK 663
    Length:663
    Mass (Da):77,083
    Last modified:July 24, 2007 - v1
    Checksum:i8FAA32F7735D97E8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAVO02000029 Genomic DNA. Translation: EDM85535.1.

    Genome annotation databases

    EnsemblBacteriaiEDM85535; EDM85535; RUMOBE_03919.
    PATRICi29798544. VBIRumObe16879_1928.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AAVO02000029 Genomic DNA. Translation: EDM85535.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3M46 X-ray 2.66 A/B 1-663 [» ]
    3M6D X-ray 2.90 A/B 1-663 [» ]
    3MKK X-ray 1.91 A/B 1-663 [» ]
    3N04 X-ray 2.02 A/B 1-663 [» ]
    3NSX X-ray 1.57 A/B 1-663 [» ]
    3NUK X-ray 2.06 A/B 1-663 [» ]
    3PHA X-ray 2.17 A/B/C/D 1-663 [» ]
    3POC X-ray 1.99 A/B 1-663 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH31. Glycoside Hydrolase Family 31.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai EDM85535 ; EDM85535 ; RUMOBE_03919 .
    PATRICi 29798544. VBIRumObe16879_1928.

    Miscellaneous databases

    EvolutionaryTracei A5ZY13.

    Family and domain databases

    InterProi IPR011013. Gal_mutarotase_SF_dom.
    IPR000322. Glyco_hydro_31.
    IPR025887. Glyco_hydro_31_N_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF13802. Gal_mutarotas_2. 1 hit.
    PF01055. Glyco_hydro_31. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 2 hits.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE.
      Strain: ATCC 29174Imported.
    2. "Draft genome sequence of Ruminococcus obeum (ATCC 29174)."
      Sudarsanam P., Ley R., Guruge J., Turnbaugh P.J., Mahowald M., Liep D., Gordon J.
      Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: ATCC 29174Imported.
    3. "Novel ?-glucosidase from human gut microbiome: substrate specificities and their switch."
      Tan K., Tesar C., Wilton R., Keigher L., Babnigg G., Joachimiak A.
      FASEB J. 24:3939-3949(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.91 ANGSTROMS) IN COMPLEX WITH GLUCOSE.
    4. "The crystal structure of the The crystal structure of the D420A mutant of the alpha-glucosidase (FAMILY 31) from Ruminococcus obeum ATCC 29174."
      Tan K., Tesar C., Wilton R., Keigher L., Babnigg G., Joachimiak A.
      Submitted (JUL-2010) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.57 ANGSTROMS).
    5. "THE CRYSTAL STRUCTURE OF THE W169Y mutant of ALPHA-GLUCOSIDASE (FAMILY 31) from RUMINOCOCCUS OBEUM ATCC 29174."
      Tan K., Tesar C., Wilton R., Keigher L., Babnigg G., Joachimiak A.
      Submitted (JUL-2010) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.06 ANGSTROMS).
    6. "The crystal structure of the D307A mutant of alpha-Glucosidase (FAMILY 31) from Ruminococcus obeum ATCC 29174 in complex with acarbose."
      Tan K., Tesar C., Wilton R., Keigher L., Babnigg G., Joachimiak A.
      Submitted (NOV-2010) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.99 ANGSTROMS).
    7. "The crystal structure of the W169Y mutant of alpha-glucosidase (gh31 family) from Ruminococcus obeum atcc 29174 in complex with acarbose."
      Tan K., Tesar C., Keigher L., Babnigg G., Joachimiak A.
      Submitted (NOV-2010) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.17 ANGSTROMS).

    Entry informationi

    Entry nameiA5ZY13_9FIRM
    AccessioniPrimary (citable) accession number: A5ZY13
    Entry historyi
    Integrated into UniProtKB/TrEMBL: July 24, 2007
    Last sequence update: July 24, 2007
    Last modified: October 1, 2014
    This is version 32 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Caution

    The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.Imported

    Keywords - Technical termi

    3D-structureImported

    External Data

    Dasty 3