Glycosyl hydrolase, family 31 - Ruminococcus obeum ATCC 29174

Preferred order of sections

Names and origin

Protein names

Submitted name:
Glycosyl hydrolase, family 31 EMBL EDM85535.1
EC=3.2.1.- EMBL EDM85535.1

Gene names

ORF Names:

RUMOBE_03919 EMBL EDM85535.1

Organism

Ruminococcus obeum ATCC 29174 EMBL EDM85535.1

Taxonomic identifier

411459 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Lachnospiraceae › Blautia ›

Protein attributes

Sequence length	663 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Caution

The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data. EMBL EDM85535.1

Ontologies

Keywords
Molecular function	Glycosidase EMBL EDM85535.1 Hydrolase
Technical term	3D-structure PDB 3NSX PDB 3NUK PDB 3M6D PDB 3M46 PDB 3MKK PDB 3PHA PDB 3N04 PDB 3POC
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	carbohydrate binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Binding site

197

1

Glucose PDB 3MKK

Binding site

404

1

Glucose PDB 3MKK

Binding site

420

1

Glucose PDB 3MKK

Binding site

478

1

Glucose PDB 3MKK

Sequences

Sequence

Length

Mass (Da)

Tools

A5ZY13 [UniParc].

Last modified July 24, 2007. Version 1.
Checksum: 8FAA32F7735D97E8
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FASTA

663

77,083

        10         20         30         40         50         60 
MIRKYRYGAP FDTEALTEKI ETAEEAFPYG EISQKEGFAF TYIMDEDDIV YGLGESNRGI 

        70         80         90        100        110        120 
NKRGYCYISN CTDDPIHTED KRSLYGAHNF IIVSGKTTFG LFFDYPSKLT FDIGYTRMDT 

       130        140        150        160        170        180 
LKVSCENADL DIYVIEGENA YDIVKQFRRV IGRSYIPPKF AFGFGQSRWG YTTKEDFRAV 

       190        200        210        220        230        240 
AKGYRENHIP IDMIYMDIDY MQDFKDFTVN EKNFPDFPEF VKEMKDQELR LIPIIDAGVK 

       250        260        270        280        290        300 
VEKGYEVYEE GVKNNYFCKR EDGSDFVAAV WPGDTHFPDM LNPEARKWFG DKYRFLIDQG 

       310        320        330        340        350        360 
IEGFWNDMNE PAIFYSSEGL AEAKEFAGEF AKDTEGKIHP WAMQAKMKDI VNSPEDYKRF 

       370        380        390        400        410        420 
YHNVNGKKIR HDKVHNLFGY NMTRAAGEAF ERIDPEKRFL MFSRSSYIGM HRYGGIWMGD 

       430        440        450        460        470        480 
NKSWWSHILL NLKMLPSLNM CGFMYTGADL GGFGDDTTRD LLLRFLALGV FTPLMRDHAA 

       490        500        510        520        530        540 
EGTREQECYQ FENIEDFRSV INARYRLVPY LYSEYMKAAL NDDMYFKPLG FVYPDDKMAI 

       550        560        570        580        590        600 
RVEDQLMLGN EIMIAPVYEQ NARGRYVYLP EEMKFIKFMP DGSISEEVLE KGVHYVDVAL 

       610        620        630        640        650        660 
NEVPLFIRSG KCIPVAEAAE CVKDIDTENM QLIGYEGSSY TLYEDDGIHK DYDKKENYRV 


LTK

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References

[1]	Fulton L., Clifton S., Fulton B., Xu J., Minx P., Pepin K.H., Johnson M., Thiruvilangam P., Bhonagiri V., Nash W.E., Mardis E.R., Wilson R.K. Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 29174 EMBL EDM85535.1.
[2]	"Draft genome sequence of Ruminococcus obeum (ATCC 29174)." Sudarsanam P., Ley R., Guruge J., Turnbaugh P.J., Mahowald M., Liep D., Gordon J. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 29174 EMBL EDM85535.1.
[3]	"Novel ?-glucosidase from human gut microbiome: substrate specificities and their switch." Tan K., Tesar C., Wilton R., Keigher L., Babnigg G., Joachimiak A. FASEB J. 24:3939-3949(2010) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.91 ANGSTROMS) IN COMPLEX WITH GLUCOSE.
[4]	"The crystal structure of the The crystal structure of the D420A mutant of the alpha-glucosidase (FAMILY 31) from Ruminococcus obeum ATCC 29174." Tan K., Tesar C., Wilton R., Keigher L., Babnigg G., Joachimiak A. Submitted (JUL-2010) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.57 ANGSTROMS).
[5]	"THE CRYSTAL STRUCTURE OF THE W169Y mutant of ALPHA-GLUCOSIDASE (FAMILY 31) from RUMINOCOCCUS OBEUM ATCC 29174." Tan K., Tesar C., Wilton R., Keigher L., Babnigg G., Joachimiak A. Submitted (JUL-2010) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.06 ANGSTROMS).
[6]	"The crystal structure of the D307A mutant of alpha-Glucosidase (FAMILY 31) from Ruminococcus obeum ATCC 29174 in complex with acarbose." Tan K., Tesar C., Wilton R., Keigher L., Babnigg G., Joachimiak A. Submitted (NOV-2010) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.99 ANGSTROMS).
[7]	"The crystal structure of the W169Y mutant of alpha-glucosidase (gh31 family) from Ruminococcus obeum atcc 29174 in complex with acarbose." Tan K., Tesar C., Keigher L., Babnigg G., Joachimiak A. Submitted (NOV-2010) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (2.17 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AAVO02000029 Genomic DNA. Translation: EDM85535.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3M46	X-ray	2.66	A/B	1-663	[»]
3M6D	X-ray	2.90	A/B	1-663	[»]
3MKK	X-ray	1.91	A/B	1-663	[»]
3N04	X-ray	2.02	A/B	1-663	[»]
3NSX	X-ray	1.57	A/B	1-663	[»]
3NUK	X-ray	2.06	A/B	1-663	[»]
3PHA	X-ray	2.17	A/B/C/D	1-663	[»]
3POC	X-ray	1.99	A/B	1-663	[»]

ModBase

Search...

Protein family/group databases

CAZy

GH31. Glycoside Hydrolase Family 31.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EDM85535; EDM85535; RUMOBE_03919.

PATRIC

29798544. VBIRumObe16879_1928.

Family and domain databases

InterPro

IPR011013. Gal_mutarotase_SF_dom.
IPR000322. Glyco_hydro_31.
IPR025887. Glyco_hydro_31_N_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]

PANTHER

PTHR22762. PTHR22762. 1 hit.

Pfam

PF13802. Gal_mutarotas_2. 1 hit.
PF01055. Glyco_hydro_31. 1 hit.
[Graphical view]

SUPFAM

SSF74650. Gal_mut_like. 1 hit.
SSF51445. Glyco_hydro_cat. 1 hit.

PROSITE

PS00129. GLYCOSYL_HYDROL_F31_1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

A5ZY13.

Entry information

Entry name

A5ZY13_9FIRM

Accession

Primary (citable) accession number: A5ZY13

Entry history

Integrated into UniProtKB/TrEMBL:	July 24, 2007
Last sequence update:	July 24, 2007
Last modified:	April 3, 2013
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)