A5X5Y0 (5HT3E_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-hydroxytryptamine receptor 3E
Short name=5-HT3-E
Short name=5-HT3E

Alternative name(s):
Serotonin receptor 3E

Gene names

Name:

HTR3E

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel.
Subunit structure	Forms a pentaheteromeric complex with HTR3A. Not functional as a homomeric complex. Ref.3
Subcellular location	Cell membrane; Multi-pass membrane protein. Note: Presumably retained within the endoplasmic reticulum unless complexed with HTR3A. Ref.3 Ref.4
Tissue specificity	Expressed in adult colon and intestine. Ref.1 Ref.2
Sequence similarities	Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3E sub-subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Ion channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ion transmembrane transport Traceable author statement. Source: Reactome
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Traceable author statement. Source: Reactome postsynaptic membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	extracellular ligand-gated ion channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: A5X5Y0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: A5X5Y0-2)

Also known as: 5-HT3c1;

The sequence of this isoform differs from the canonical sequence as follows:
     79-93: Missing.

Isoform 3 (identifier: A5X5Y0-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: MEGSWFHRKRFSFYLLLGFLLQ → MLAFILSRATPRPALGPLSYREHRVALLHLTHSMSTT

Isoform 4 (identifier: A5X5Y0-4)

Also known as: 5-HT3c1 long;

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: MEGSWFHRKRFSFYLLLGFLLQ → MLAFILSRATPRPALGPLSYREHRVALLHLTHSMSTT
     79-93: Missing.

Isoform 5 (identifier: A5X5Y0-6)

Also known as: HTR3E_V3;

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: MEGSWFHRKRFSFYLLLGFLLQ → MLAFILSRATPRPALGPLSYREHRVALLHLTHSMSTT
     79-93: Missing.
     129-129: E → ELCVSRAGQREVPSPGSHRDHSLPLGP

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 456	431	5-hydroxytryptamine receptor 3E		PRO_0000312294
Regions
	Topological domain	26 – 248	223	Extracellular Potential
	Transmembrane	249 – 269	21	Helical; Name=1; Potential
	Topological domain	270 – 282	13	Cytoplasmic Potential
	Transmembrane	283 – 303	21	Helical; Name=2; Potential
	Topological domain	304 – 307	4	Extracellular Potential
	Transmembrane	308 – 328	21	Helical; Name=3; Potential
	Topological domain	329 – 433	105	Cytoplasmic Potential
	Transmembrane	434 – 454	21	Helical; Name=4; Potential
	Topological domain	455 – 456	2	Extracellular Potential
Amino acid modifications
	Glycosylation	175	1	N-linked (GlcNAc...) Potential
	Disulfide bond	162 ↔ 176		By similarity
Natural variations
	Alternative sequence	1 – 22	22	MEGSW…GFLLQ → MLAFILSRATPRPALGPLSY REHRVALLHLTHSMSTT in isoform 3, isoform 4 and isoform 5.		VSP_029803
	Alternative sequence	79 – 93	15	Missing in isoform 2, isoform 4 and isoform 5.		VSP_029804
	Alternative sequence	129	1	E → ELCVSRAGQREVPSPGSHRD HSLPLGP in isoform 5.		VSP_045573
	Natural variant	71	1	A → T. Ref.4 Corresponds to variant rs7627615 [ dbSNP | Ensembl ].		VAR_037481
	Natural variant	430	1	A → T. Corresponds to variant rs13324468 [ dbSNP | Ensembl ].		VAR_037482
Experimental info
	Sequence conflict	44	1	A → T in AAI01183. Ref.6
	Sequence conflict	44	1	A → T in AAI01184. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 10, 2007. Version 1. Checksum: 4BFA6561BA309E83	FASTA	456	51,438
	10 20 30 40 50 60 MEGSWFHRKR FSFYLLLGFL LQGRGVTFTI NCSGFGQHGA DPTALNSVFN RKPFRPVTNI 70 80 90 100 110 120 SVPTQVNISF AMSAILDVNE QLHLLSSFLW LEMVWDNPFI SWNPEECEGI TKMSMAAKNL 130 140 150 160 170 180 WLPDIFIIEL MDVDKTPKGL TAYVSNEGRI RYKKPMKVDS ICNLDIFYFP FDQQNCTLTF 190 200 210 220 230 240 SSFLYTVDSM LLDMEKEVWE ITDASRNILQ THGEWELLGL SKATAKLSRG GNLYDQIVFY 250 260 270 280 290 300 VAIRRRPSLY VINLLVPSGF LVAIDALSFY LPVKSGNRVP FKITLLLGYN VFLLMMSDLL 310 320 330 340 350 360 PTSGTPLIGV YFALCLSLMV GSLLETIFIT HLLHVATTQP PPLPRWLHSL LLHCNSPGRC 370 380 390 400 410 420 CPTAPQKENK GPGLTPTHLP GVKEPEVSAG QMPGPAEAEL TGGSEWTRAQ REHEAQKQHS 430 440 450 VELWLQFSHA MDAMLFRLYL LFMASSIITV ICLWNT
	Isoform 2 (5-HT3c1) [UniParc]. Checksum: F604941E9F3849D6	FASTA	441	49,596
	10 20 30 40 50 60 MEGSWFHRKR FSFYLLLGFL LQGRGVTFTI NCSGFGQHGA DPTALNSVFN RKPFRPVTNI 70 80 90 100 110 120 SVPTQVNISF AMSAILDVVW DNPFISWNPE ECEGITKMSM AAKNLWLPDI FIIELMDVDK 130 140 150 160 170 180 TPKGLTAYVS NEGRIRYKKP MKVDSICNLD IFYFPFDQQN CTLTFSSFLY TVDSMLLDME 190 200 210 220 230 240 KEVWEITDAS RNILQTHGEW ELLGLSKATA KLSRGGNLYD QIVFYVAIRR RPSLYVINLL 250 260 270 280 290 300 VPSGFLVAID ALSFYLPVKS GNRVPFKITL LLGYNVFLLM MSDLLPTSGT PLIGVYFALC 310 320 330 340 350 360 LSLMVGSLLE TIFITHLLHV ATTQPPPLPR WLHSLLLHCN SPGRCCPTAP QKENKGPGLT 370 380 390 400 410 420 PTHLPGVKEP EVSAGQMPGP AEAELTGGSE WTRAQREHEA QKQHSVELWL QFSHAMDAML 430 440 FRLYLLFMAS SIITVICLWN T
	Isoform 3 [UniParc]. Checksum: 94A7B8D13492E8F1	FASTA	471	52,808
	10 20 30 40 50 60 MLAFILSRAT PRPALGPLSY REHRVALLHL THSMSTTGRG VTFTINCSGF GQHGADPTAL 70 80 90 100 110 120 NSVFNRKPFR PVTNISVPTQ VNISFAMSAI LDVNEQLHLL SSFLWLEMVW DNPFISWNPE 130 140 150 160 170 180 ECEGITKMSM AAKNLWLPDI FIIELMDVDK TPKGLTAYVS NEGRIRYKKP MKVDSICNLD 190 200 210 220 230 240 IFYFPFDQQN CTLTFSSFLY TVDSMLLDME KEVWEITDAS RNILQTHGEW ELLGLSKATA 250 260 270 280 290 300 KLSRGGNLYD QIVFYVAIRR RPSLYVINLL VPSGFLVAID ALSFYLPVKS GNRVPFKITL 310 320 330 340 350 360 LLGYNVFLLM MSDLLPTSGT PLIGVYFALC LSLMVGSLLE TIFITHLLHV ATTQPPPLPR 370 380 390 400 410 420 WLHSLLLHCN SPGRCCPTAP QKENKGPGLT PTHLPGVKEP EVSAGQMPGP AEAELTGGSE 430 440 450 460 470 WTRAQREHEA QKQHSVELWL QFSHAMDAML FRLYLLFMAS SIITVICLWN T
	Isoform 4 (5-HT3c1 long) [UniParc]. Checksum: 81FD94F996ED344D	FASTA	456	50,965
	10 20 30 40 50 60 MLAFILSRAT PRPALGPLSY REHRVALLHL THSMSTTGRG VTFTINCSGF GQHGADPTAL 70 80 90 100 110 120 NSVFNRKPFR PVTNISVPTQ VNISFAMSAI LDVVWDNPFI SWNPEECEGI TKMSMAAKNL 130 140 150 160 170 180 WLPDIFIIEL MDVDKTPKGL TAYVSNEGRI RYKKPMKVDS ICNLDIFYFP FDQQNCTLTF 190 200 210 220 230 240 SSFLYTVDSM LLDMEKEVWE ITDASRNILQ THGEWELLGL SKATAKLSRG GNLYDQIVFY 250 260 270 280 290 300 VAIRRRPSLY VINLLVPSGF LVAIDALSFY LPVKSGNRVP FKITLLLGYN VFLLMMSDLL 310 320 330 340 350 360 PTSGTPLIGV YFALCLSLMV GSLLETIFIT HLLHVATTQP PPLPRWLHSL LLHCNSPGRC 370 380 390 400 410 420 CPTAPQKENK GPGLTPTHLP GVKEPEVSAG QMPGPAEAEL TGGSEWTRAQ REHEAQKQHS 430 440 450 VELWLQFSHA MDAMLFRLYL LFMASSIITV ICLWNT
	Isoform 5 (HTR3E_V3) [UniParc]. Checksum: DDE59C2099D78219	FASTA	482	53,700
	10 20 30 40 50 60 MLAFILSRAT PRPALGPLSY REHRVALLHL THSMSTTGRG VTFTINCSGF GQHGADPTAL 70 80 90 100 110 120 NSVFNRKPFR PVTNISVPTQ VNISFAMSAI LDVVWDNPFI SWNPEECEGI TKMSMAAKNL 130 140 150 160 170 180 WLPDIFIIEL CVSRAGQREV PSPGSHRDHS LPLGPLMDVD KTPKGLTAYV SNEGRIRYKK 190 200 210 220 230 240 PMKVDSICNL DIFYFPFDQQ NCTLTFSSFL YTVDSMLLDM EKEVWEITDA SRNILQTHGE 250 260 270 280 290 300 WELLGLSKAT AKLSRGGNLY DQIVFYVAIR RRPSLYVINL LVPSGFLVAI DALSFYLPVK 310 320 330 340 350 360 SGNRVPFKIT LLLGYNVFLL MMSDLLPTSG TPLIGVYFAL CLSLMVGSLL ETIFITHLLH 370 380 390 400 410 420 VATTQPPPLP RWLHSLLLHC NSPGRCCPTA PQKENKGPGL TPTHLPGVKE PEVSAGQMPG 430 440 450 460 470 480 PAEAELTGGS EWTRAQREHE AQKQHSVELW LQFSHAMDAM LFRLYLLFMA SSIITVICLW NT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, physical mapping and expression analysis of the human 5-HT3 serotonin receptor-like genes HTR3C, HTR3D and HTR3E." Niesler B., Frank B., Kapeller J., Rappold G.A. Gene 310:101-111(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3), TISSUE SPECIFICITY.
[2]	"A cluster of novel serotonin receptor 3-like genes on human chromosome 3." Karnovsky A.M., Gotow L.F., McKinley D.D., Piechan J.L., Ruble C.L., Mills C.J., Schellin K.A.B., Slightom J.L., Fitzgerald L.R., Benjamin C.W., Roberds S.L. Gene 319:137-148(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 2 AND 4), TISSUE SPECIFICITY.
[3]	"Characterization of the novel human serotonin receptor subunits 5-HT3C, 5-HT3D, and 5-HT3E." Niesler B., Walstab J., Combrink S., Moeller D., Kapeller J., Rietdorf J., Boenisch H., Goethert M., Rappold G., Bruess M. Mol. Pharmacol. 72:8-17(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), SUBUNIT, SUBCELLULAR LOCATION.
[4]	"Characterisation of 5-HT3C, 5-HT3D and 5-HT3E receptor subunits: evolution, distribution and function." Holbrook J.D., Gill C.H., Zebda N., Spencer J.P., Leyland R., Rance K.H., Trinh H., Balmer G., Kelly F.M., Yusaf S.P., Courtenay N., Luck J., Rhodes A., Modha S., Moore S.E., Sanger G.J., Gunthorpe M.J. J. Neurochem. 108:384-396(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5), ALTERNATIVE SPLICING, SUBCELLULAR LOCATION, VARIANT THR-71.
[5]	"The DNA sequence, annotation and analysis of human chromosome 3." Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J. , Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P., Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H., Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J., Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W., Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B., Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H., Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J., Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X., Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R., Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A. Nature 440:1194-1198(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY349352 mRNA. Translation: AAQ93476.1. AY349353 mRNA. Translation: AAQ93477.1. AY159813 mRNA. Translation: AAO38167.2. DQ644022 mRNA. Translation: ABG35128.1. EU165354 mRNA. Translation: ABW05298.1. AC131235 Genomic DNA. No translation available. BC101182 mRNA. Translation: AAI01183.1. BC101183 mRNA. Translation: AAI01184.1. BC101185 mRNA. Translation: AAI01186.1.
IPI	IPI00376255. IPI00876853. IPI00877063. IPI00877086. IPI00877142.
RefSeq	NP_001243542.1. NM_001256613.1. NP_001243543.1. NM_001256614.1. NP_872395.2. NM_182589.2. NP_938055.1. NM_198313.2. NP_938056.1. NM_198314.2.
UniGene	Hs.449179.
3D structure databases
ProteinModelPortal	A5X5Y0.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000335511.
Proteomic databases
PaxDb	A5X5Y0.
PRIDE	A5X5Y0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000335304; ENSP00000335511; ENSG00000186038. ENST00000415389; ENSP00000401444; ENSG00000186038. ENST00000425359; ENSP00000401900; ENSG00000186038. ENST00000436361; ENSP00000395833; ENSG00000186038. ENST00000440596; ENSP00000406050; ENSG00000186038.
GeneID	285242.
KEGG	hsa:285242.
UCSC	uc003fml.4. human. uc003fmm.3. human. uc003fmn.3. human. uc010hxq.3. human.
Organism-specific databases
CTD	285242.
GeneCards	GC03P183814.
HGNC	HGNC:24005. HTR3E.
MIM	610123. gene.
neXtProt	NX_A5X5Y0.
PharmGKB	PA134900226.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG316889.
HOGENOM	HOG000241519.
HOVERGEN	HBG106638.
InParanoid	A5X5Y0.
KO	K04819.
OrthoDB	EOG4R5034.
Enzyme and pathway databases
Reactome	REACT_15518. Transmembrane transport of small molecules.
Gene expression databases
ArrayExpress	A5X5Y0.
CleanEx	HS_HTR3E.
Genevestigator	A5X5Y0.
Family and domain databases
Gene3D	2.70.170.10. 1 hit.
InterPro	IPR006202. Neur_chan_lig-bd. IPR006201. Neur_channel. IPR006029. Neurotrans-gated_channel_TM. IPR018000. Neurotransmitter_ion_chnl_CS. [Graphical view]
PANTHER	PTHR18945. PTHR18945. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 1 hit. [Graphical view]
PRINTS	PR00252. NRIONCHANNEL.
SUPFAM	SSF90112. Neu_channel_TM. 1 hit. SSF63712. Neur_chan_LBD. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	A5X5Y0.
ChEMBL	CHEMBL3812.
GenomeRNAi	285242.
NextBio	95380.
SOURCE	Search...

Entry information

Entry name

5HT3E_HUMAN

Accession

Primary (citable) accession number: A5X5Y0
Secondary accession number(s): A8IKD7 , E9PGF1, Q495G1, Q495G3, Q6V706, Q6V707, Q7Z6B2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 4, 2007
Last sequence update:	July 10, 2007
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.