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Protein

E3 ubiquitin-protein ligase CHFR

Gene

chfr

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating ubc13-mms2 (ube2n-ube2v2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri277 – 31640RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri606 – 62823PBZ-typeAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase CHFR (EC:6.3.2.-)
Alternative name(s):
Checkpoint with forkhead and RING finger domains protein
Gene namesi
Name:chfr
ORF Names:si:dkey-69h6.7
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 5

Organism-specific databases

ZFINiZDB-GENE-030131-3522. chfr.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 637637E3 ubiquitin-protein ligase CHFRPRO_0000385302Add
BLAST

Proteomic databases

PaxDbiA5WW08.

Expressioni

Gene expression databases

BgeeiA5WW08.

Interactioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000099431.

Structurei

3D structure databases

ProteinModelPortaliA5WW08.
SMRiA5WW08. Positions 8-117.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 8252FHAPROSITE-ProRule annotationAdd
BLAST

Domaini

The PBZ-type zinc finger (also named CYR) mediates non-covalent poly(ADP-ribose)-binding. Poly(ADP-ribose)-binding is dependent on the presence of zinc and is required for its function in antephase checkpoint (By similarity).By similarity
The FHA domain plays a key role in the anti-proliferative properties of the protein and is involved in initiating a cell cycle arrest at G2/M.By similarity

Sequence similaritiesi

Belongs to the CHFR family.Curated
Contains 1 FHA domain.PROSITE-ProRule annotation
Contains 1 PBZ-type zinc finger.Curated
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri277 – 31640RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri606 – 62823PBZ-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0802. Eukaryota.
COG5243. LUCA.
GeneTreeiENSGT00400000022306.
HOGENOMiHOG000290186.
InParanoidiA5WW08.
KOiK10644.
OMAiELAYQYR.
OrthoDBiEOG78D7JS.
PhylomeDBiA5WW08.
TreeFamiTF330957.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR000253. FHA_dom.
IPR008984. SMAD_FHA_domain.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00498. FHA. 1 hit.
[Graphical view]
SMARTiSM00240. FHA. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A5WW08-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTNQDSDQAW GKLVKVDASP GSEIVLINSE CTVGRKKDCD LSFPANKLVS
60 70 80 90 100
GNHCKITHDQ NSGKVWLEDM STNGTVINMS KVVKKQTHLL QNGDVIYFVY
110 120 130 140 150
RKNEPEQNIA YVYQSITPQE SASHDVEDAG REEDSDLTET ESEPAPVEPV
160 170 180 190 200
IVKPLPQSGH EDPQPSTSSS SLHFYNMPLS TCSDVSARKN PVSSSAVCKG
210 220 230 240 250
DSTSSGSPAQ TRLKWTCWTD GEPEEEMQRK RRKTDRDDPG FGSAHSDASA
260 270 280 290 300
DIPLRGASGK EKTEGATTDK MEESLTCIIC QDLLYDCISV QPCMHTFCAA
310 320 330 340 350
CYSGWMERSS FCPTCRCPVE RIRKNHILNN LVEAYLLQHP EKCRTEDDLR
360 370 380 390 400
SMDARNKITQ DMLQPKVERS FSDEEASSDY LFELSDNDSD ISDMSQPYMM
410 420 430 440 450
CRQCPGYRKE LSSALWICES AQSESLAKTA GDGPSTSSDS TTAAPQEFRC
460 470 480 490 500
PPQASHLICT CCLQPMPDRR FEHLPPQVSP QHCLVCQKPF CHVYWGCPRI
510 520 530 540 550
GCHGCLARFS ELNLNDKCLD GVFNGNQYES EVLQNYLSCR GMSWRHLLQD
560 570 580 590 600
SLQALQQGLY HLSDYRITAN SFLCYCCGLR TFRELAYKYR ERIPPSELPD
610 620 630
AVTNRPNCYW GRNCRTQVKA HHALKFNHIC EQTRFKN
Length:637
Mass (Da):71,730
Last modified:July 10, 2007 - v1
Checksum:i7193C3203671217E
GO
Isoform 2 (identifier: A5WW08-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     443-443: Missing.

Show »
Length:636
Mass (Da):71,659
Checksum:iB42C312A4EC8230B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti61 – 611N → I in AAI53312 (Ref. 2) Curated
Sequence conflicti64 – 641K → Q in AAI53312 (Ref. 2) Curated
Sequence conflicti176 – 1761N → I in AAI53312 (Ref. 2) Curated
Sequence conflicti200 – 2001G → R in AAI53312 (Ref. 2) Curated
Sequence conflicti204 – 2041S → P in AAI53312 (Ref. 2) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei443 – 4431Missing in isoform 2. 1 PublicationVSP_038139

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT573109 Genomic DNA. Translation: CAN88580.1.
BC153311 mRNA. Translation: AAI53312.1.
RefSeqiNP_001093485.1. NM_001100015.1. [A5WW08-1]
XP_005165129.1. XM_005165072.2. [A5WW08-2]
UniGeneiDr.148986.

Genome annotation databases

EnsembliENSDART00000110783; ENSDARP00000099431; ENSDARG00000075347. [A5WW08-1]
ENSDART00000115227; ENSDARP00000098332; ENSDARG00000075347. [A5WW08-1]
GeneIDi564271.
KEGGidre:564271.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CT573109 Genomic DNA. Translation: CAN88580.1.
BC153311 mRNA. Translation: AAI53312.1.
RefSeqiNP_001093485.1. NM_001100015.1. [A5WW08-1]
XP_005165129.1. XM_005165072.2. [A5WW08-2]
UniGeneiDr.148986.

3D structure databases

ProteinModelPortaliA5WW08.
SMRiA5WW08. Positions 8-117.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000099431.

Proteomic databases

PaxDbiA5WW08.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000110783; ENSDARP00000099431; ENSDARG00000075347. [A5WW08-1]
ENSDART00000115227; ENSDARP00000098332; ENSDARG00000075347. [A5WW08-1]
GeneIDi564271.
KEGGidre:564271.

Organism-specific databases

CTDi55743.
ZFINiZDB-GENE-030131-3522. chfr.

Phylogenomic databases

eggNOGiKOG0802. Eukaryota.
COG5243. LUCA.
GeneTreeiENSGT00400000022306.
HOGENOMiHOG000290186.
InParanoidiA5WW08.
KOiK10644.
OMAiELAYQYR.
OrthoDBiEOG78D7JS.
PhylomeDBiA5WW08.
TreeFamiTF330957.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

NextBioi20885304.
PROiA5WW08.

Gene expression databases

BgeeiA5WW08.

Family and domain databases

Gene3Di2.60.200.20. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR000253. FHA_dom.
IPR008984. SMAD_FHA_domain.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PfamiPF00498. FHA. 1 hit.
[Graphical view]
SMARTiSM00240. FHA. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
PROSITEiPS50006. FHA_DOMAIN. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The zebrafish reference genome sequence and its relationship to the human genome."
    Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
    , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
    Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Tuebingen.
  2. NIH - Zebrafish Gene Collection (ZGC) project
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Testis.

Entry informationi

Entry nameiCHFR_DANRE
AccessioniPrimary (citable) accession number: A5WW08
Secondary accession number(s): A8E4S5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: July 10, 2007
Last modified: May 11, 2016
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.