A5WRF8 (A5WRF8_MYCTF) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605
EC=2.1.1.228 HAMAP MF_00605

Alternative name(s):
M1G-methyltransferase HAMAP MF_00605
tRNA [GM37] methyltransferase HAMAP MF_00605

Gene names

Name:	trmD HAMAP MF_00605
Ordered Locus Names:	TBFG_12920

Organism

Mycobacterium tuberculosis (strain F11) [Complete proteome] [HAMAP]

Taxonomic identifier

336982 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	230 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity. HAMAP MF_00605 RuleBase RU003464
Catalytic activity	S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA. HAMAP MF_00605 S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. RuleBase RU003464
Subunit structure	Homodimer By similarity. HAMAP MF_00605 RuleBase RU003464
Subcellular location	Cytoplasm By similarity HAMAP MF_00605 RuleBase RU003464.
Sequence similarities	Belongs to the RNA methyltransferase TrmD family. HAMAP MF_00605 RuleBase RU003464

Ontologies

Keywords
Biological process	tRNA processing HAMAP MF_00605 RuleBase RU003464
Cellular component	Cytoplasm HAMAP MF_00605 RuleBase RU003464
Ligand	S-adenosyl-L-methionine HAMAP MF_00605 RuleBase RU003464
Molecular function	Methyltransferase HAMAP MF_00605 RuleBase RU003464 EMBL ABR07247.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	133 – 138	6	S-adenosyl-L-methionine binding By similarity HAMAP MF_00605
Sites
	Binding site	109	1	S-adenosyl-L-methionine; via amide nitrogen By similarity HAMAP MF_00605

Sequences

	Sequence		Length	Mass (Da)
	A5WRF8 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: 5981C3D149ED0AC5	FASTA	230	25,166
	10 20 30 40 50 60 MRIDIVTIFP ACLDPLRQSL PGKAIESGLV DLNVHDLRRW THDVHHSVDD APYGGGPGMV 70 80 90 100 110 120 MKAPVWGEAL DEICSSETLL IVPTPAGVLF TQATAQRWTT ESHLVFACGR YEGIDQRVVQ 130 140 150 160 170 180 DAARRMRVEE VSIGDYVLPG GESAAVVMVE AVLRLLAGVL GNPASHQDDS HSTGLDGLLE 190 200 210 220 230 GPSYTRPASW RGLDVPEVLL SGDHARIAAW RREVSLQRTR ERRPDLSHPD

References

[1]

"The complete genome sequence of Mycobacterium tuberculosis F11." Birren B., Lander E., Galagan J., Devon K., Nusbaum C., Borowsky M.L., Grabherr M., Mauceli E., Brockman W., Young S., LaButti K., Pushparaj V., Sykes S., Baldwin J., Fitzgerald M., Bloom T., Zimmer A., Settipalli S. , Shea T., Arachchi H., Macdonald P., Abouelleil A., Lui A., Priest M., Berlin A., Gearin G., Brown A., Aftuck L., Bessette D., Allen N., Lubonja R., Lokyitsang T., Matthews C., Dunbar C., Benamara M., Nguyen T., Negash T., DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Kodira C., Zeng Q., Yandava C., O'Leary S., Alvarado L., Victor T., Murray M. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000717 Genomic DNA. Translation: ABR07247.1.
RefSeq	YP_001288849.1. NC_009565.1.
3D structure databases
ProteinModelPortal	A5WRF8.
ModBase	Search...
Protein-protein interaction databases
STRING	A5WRF8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5223606.
GenomeReviews	Gene locus TBFG_12920 in contig CP000717_GR.
KEGG	mtf:TBFG_12920.
PATRIC	18137247. VBIMycTub9078_3260.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VCGRFEG.
ProtClustDB	PRK00026.
Family and domain databases
HAMAP	MF_00605. TrmD. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. IPR023148. tRNA_m1G_MeTrfase_C. [Graphical view]
Gene3D	G3DSA:1.10.1270.20. tRNA_m1G_MeTrfase_C. 1 hit.
KO	K00554.
PANTHER	PTHR10056. PTHR10056. 1 hit.
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
TIGRFAMs	TIGR00088. TrmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A5WRF8_MYCTF

Accession

Primary (citable) accession number: A5WRF8

Entry history

Integrated into UniProtKB/TrEMBL:	July 28, 2009
Last sequence update:	July 28, 2009
Last modified:	December 14, 2011
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)