A5WRF4 (A5WRF4_MYCTF) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Ribonuclease HII HAMAP MF_00052
Short name=RNase HII HAMAP MF_00052
EC=3.1.26.4 HAMAP MF_00052

Gene names

Name:	rnhB HAMAP MF_00052
Ordered Locus Names:	TBFG_12916

Organism

Mycobacterium tuberculosis (strain F11) [Complete proteome] [HAMAP]

Taxonomic identifier

336982 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. RuleBase RU003515 HAMAP MF_00052
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. RuleBase RU003515 HAMAP MF_00052
Cofactor	Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity. HAMAP MF_00052
Subcellular location	Cytoplasm By similarity HAMAP MF_00052.
Sequence similarities	Belongs to the RNase HII family. RuleBase RU003515 HAMAP MF_00052

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP MF_00052
Ligand	Manganese HAMAP MF_00052 Metal-binding HAMAP MF_00052
Molecular function	Endonuclease RuleBase RU003515 HAMAP MF_00052 Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	manganese ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro ribonuclease H activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	39	1	Divalent metal cation By similarity HAMAP MF_00052
	Metal binding	40	1	Divalent metal cation By similarity HAMAP MF_00052
	Metal binding	133	1	Divalent metal cation By similarity HAMAP MF_00052

Sequences

	Sequence		Length	Mass (Da)
	A5WRF4 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: 786F0E790188279F	FASTA	264	27,646
	10 20 30 40 50 60 MTKTWPPRTV IRKSGGLRGM RTLESALHRG GLGPVAGVDE VGRGACAGPL VVAACVLGPG 70 80 90 100 110 120 RIASLAALDD SKKLSEQARE KLFPLICRYA VAYHVVFIPS AEVDRRGVHV ANIEGMRRAV 130 140 150 160 170 180 AGLAVRPGYV LSDGFRVPGL PMPSLPVIGG DAAAACIAAA SVLAKVSRDR VMVALDADHP 190 200 210 220 230 240 GYGFAEHKGY STPAHSRALA RLGPCPQHRY SFINVRRVAS GSNTAEVADG QPDPRDGTAQ 250 260 TGEGRWSKSS HPATMRATGR AQGT

References

[1]

"The complete genome sequence of Mycobacterium tuberculosis F11." Birren B., Lander E., Galagan J., Devon K., Nusbaum C., Borowsky M.L., Grabherr M., Mauceli E., Brockman W., Young S., LaButti K., Pushparaj V., Sykes S., Baldwin J., Fitzgerald M., Bloom T., Zimmer A., Settipalli S. , Shea T., Arachchi H., Macdonald P., Abouelleil A., Lui A., Priest M., Berlin A., Gearin G., Brown A., Aftuck L., Bessette D., Allen N., Lubonja R., Lokyitsang T., Matthews C., Dunbar C., Benamara M., Nguyen T., Negash T., DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Kodira C., Zeng Q., Yandava C., O'Leary S., Alvarado L., Victor T., Murray M. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000717 Genomic DNA. Translation: ABR07243.1.
RefSeq	YP_001288845.1. NC_009565.1.
3D structure databases
ProteinModelPortal	A5WRF4.
ModBase	Search...
Protein-protein interaction databases
STRING	A5WRF4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5223602.
GenomeReviews	Gene locus TBFG_12916 in contig CP000717_GR.
KEGG	mtf:TBFG_12916.
PATRIC	18137239. VBIMycTub9078_3256.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	RLGPTPI.
ProtClustDB	PRK00015.
Family and domain databases
HAMAP	MF_00052_B. RNase_HII_B. [Tree]
InterPro	IPR022898. RNase_HII. IPR024567. RNase_HII/HIII_dom. IPR012337. RNaseH-like_dom. [Graphical view]
KO	K03470.
Pfam	PF01351. RNase_HII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A5WRF4_MYCTF

Accession

Primary (citable) accession number: A5WRF4

Entry history

Integrated into UniProtKB/TrEMBL:	July 28, 2009
Last sequence update:	July 28, 2009
Last modified:	December 14, 2011
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)