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A5WR98 (A5WR98_MYCTF) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Proline--tRNA ligase HAMAP MF_01569
EC=6.1.1.15 HAMAP MF_01569
Alternative name(s):
Prolyl-tRNA synthetase HAMAP MF_01569
Gene names
Name:proS HAMAP MF_01569
Ordered Locus Names:TBFG_12860
OrganismMycobacterium tuberculosis (strain F11) [Complete proteome] [HAMAP]
Taxonomic identifier336982 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length582 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity. HAMAP MF_01569

Catalytic activity

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569

Subunit structure

Homodimer By similarity. HAMAP MF_01569

Subcellular location

Cytoplasm By similarity HAMAP MF_01569.

Domain

Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity. HAMAP MF_01569

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily. HAMAP MF_01569

Ontologies

Keywords
   Biological processProtein biosynthesis HAMAP MF_01569
   Cellular componentCytoplasm HAMAP MF_01569
   LigandATP-binding HAMAP MF_01569
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase HAMAP MF_01569 EMBL ABR07187.1
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processprolyl-tRNA aminoacylation

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: HAMAP

proline-tRNA ligase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequences

Sequence LengthMass (Da)Tools
A5WR98 [UniParc].

Last modified July 28, 2009. Version 1.
Checksum: 5208D4ACBA49CDCA

FASTA58263,302
        10         20         30         40         50         60 
MITRMSELFL RTLRDDPADA EVASHKLLIR AGYIRPVAPG LYSWLPLGLR VLRNIERVIR 

        70         80         90        100        110        120 
DEMNAIGGQE ILFPALLPRA PYETTNRWTQ YGDSVFRLKD RRGNDYLLGP THEELFTLTV 

       130        140        150        160        170        180 
KGEYSSYKDF PLTLYQIQTK YRDEARPRAG ILRAREFVMK DSYSFDIDAA GLKAAYHAHR 

       190        200        210        220        230        240 
EAYQRIFDRL QVRYVIVSAV SGAMGGSASE EFLAESPSGE DAFVRCLESG YAANVEAVVT 

       250        260        270        280        290        300 
ARPDTLPIDG LPEAVVHDTG DTPTIASLVA WANEADLGRT VTAADTLKNV LIKVRQPGGD 

       310        320        330        340        350        360 
TELLAIGVPG DREVDDKRLG AALEPADYAL LDDDDFAKHP FLVKGYIGPK ALRENNVRYL 

       370        380        390        400        410        420 
VDPRIVDGTS WITGADQPGR HVVGLVAGRD FTADGTIEAA EVREGDPSPD GAGPLVMARG 

       430        440        450        460        470        480 
IEIGHIFQLG SKYTDAFTAD VLGEDGKPVR LTMGSYGIGV SRLVAVVAEQ HHDELGLRWP 

       490        500        510        520        530        540 
STVAPFDVHL VIANKDAQAR AGATALAADL DRLGVEVLLD DRQASPGVKF KDAELLGMPW 

       550        560        570        580 
IVVVGRGWAD GVVELRDRFS GQTRELVAGA SLATDIAAAV TG

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000717 Genomic DNA. Translation: ABR07187.1.
RefSeqYP_001288789.1. NC_009565.1.

3D structure databases

ProteinModelPortalA5WR98.
ModBaseSearch...

Protein-protein interaction databases

STRINGA5WR98.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5223546.
GenomeReviewsGene locus TBFG_12860 in contig CP000717_GR.
KEGGmtf:TBFG_12860.
PATRIC18137109. VBIMycTub9078_3191.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAIQPAELW.
ProtClustDBPRK09194.

Family and domain databases

HAMAPMF_01569. Pro_tRNA_synth_type1.
[Tree]
InterProIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-synth_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view]
Gene3DG3DSA:3.40.50.800. Anticodon_bd. 1 hit.
G3DSA:3.90.960.10. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
KOK01881.
PANTHERPTHR11451:SF3. PTHR11451:SF3. 1 hit.
PfamPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. YbaK. 1 hit.
[Graphical view]
PRINTSPR01046. TRNASYNTHPRO.
SUPFAMSSF52954. Anticodon_bd. 1 hit.
SSF55826. YbaK/aa-tRNA-synth-assoc-reg. 1 hit.
TIGRFAMsTIGR00409. ProS_fam_II. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA5WR98_MYCTF
AccessionPrimary (citable) accession number: A5WR98
Entry history
Integrated into UniProtKB/TrEMBL: July 28, 2009
Last sequence update: July 28, 2009
Last modified: January 25, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequences·References·Cross-refs·Entry info