A5WMP8 (A5WMP8_MYCTF) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malto-oligosyltrehalose trehalohydrolase PIRNR PIRNR006337
Short name=MTHase PIRNR PIRNR006337
EC=3.2.1.141 PIRNR PIRNR006337

Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase PIRNR PIRNR006337
Maltooligosyl trehalose trehalohydrolase PIRNR PIRNR006337

Gene names

Ordered Locus Names:

TBFG_11594

Organism

Mycobacterium tuberculosis (strain F11) [Complete proteome] [HAMAP]

Taxonomic identifier

336982 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	580 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan. PIRNR PIRNR006337
Pathway	Glycan biosynthesis; trehalose biosynthesis. PIRNR PIRNR006337
Subcellular location	Cytoplasm By similarity PIRSR PIRSR006337-1.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. PIRNR PIRNR006337

Ontologies

Keywords
Molecular function	Glycosidase PIRNR PIRNR006337 Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	247	1	Nucleophile By similarity PIRSR PIRSR006337-1
	Active site	284	1	Proton donor By similarity PIRSR PIRSR006337-1
	Site	380	1	Transition state stabilizer By similarity PIRSR PIRSR006337-3

Sequences

	Sequence		Length	Mass (Da)
	A5WMP8 [UniParc]. Last modified July 28, 2009. Version 1. Checksum: 506190468F44B862	FASTA	580	64,077
	10 20 30 40 50 60 MPEFRVWAPK PALVRLDVNG AVHAMTRSAD GWWHTTVAAP ADARYGYLLD DDPTVLPDPR 70 80 90 100 110 120 SARQPDGVHA RSQRWEPPGQ FGAARTDTGW PGRSVEGAVI YELHIGTFTT AGTFDAAIEK 130 140 150 160 170 180 LDYLVDLGID FVELMPVNSF AGTRGWGYDG VLWYSVHEPY GGPDGLVRFI DACHARRLGV 190 200 210 220 230 240 LIDAVFNHLG PSGNYLPRFG PYLSSASNPW GDGINIAGAD SDEVRHYIID CALRWMRDFH 250 260 270 280 290 300 ADGLRLDAVH ALVDTTAVHV LEELANATRW LSGQLGRPLS LIAETDRNDP RLITRPSHGG 310 320 330 340 350 360 YGITAQWNDD IHHAIHTAVS GERQGYYADF GSLATLAYTL RNGYFHAGTY SSFRRRRHGR 370 380 390 400 410 420 ALDTSAIPAT RLLAYTCTHD QVGNRALGDR PSQYLTGGQL AIKAALTLGS PYTAMLFMGE 430 440 450 460 470 480 EWGASSPFQF FCSHPEPELA HSTVAGRKEE FAEHGWAADD IPDPQDPQTF QRCKLNWAEA 490 500 510 520 530 540 GSGEHARLHR FYRDLIALRH NEADLADPWL DHLMVDYDEQ QRWVVMRRGQ LMIACNLGAE 550 560 570 580 PTCVPVSGEL VLAWESPIIG DNSTELAAYS LAILRAAEPA

References

[1]

"The complete genome sequence of Mycobacterium tuberculosis F11." Birren B., Lander E., Galagan J., Devon K., Nusbaum C., Borowsky M.L., Grabherr M., Mauceli E., Brockman W., Young S., LaButti K., Pushparaj V., Sykes S., Baldwin J., Fitzgerald M., Bloom T., Zimmer A., Settipalli S. , Shea T., Arachchi H., Macdonald P., Abouelleil A., Lui A., Priest M., Berlin A., Gearin G., Brown A., Aftuck L., Bessette D., Allen N., Lubonja R., Lokyitsang T., Matthews C., Dunbar C., Benamara M., Nguyen T., Negash T., DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P., Pearson M., Howarth C., Kodira C., Zeng Q., Yandava C., O'Leary S., Alvarado L., Victor T., Murray M. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000717 Genomic DNA. Translation: ABR05937.1.
RefSeq	YP_001287539.1. NC_009565.1.
3D structure databases
ProteinModelPortal	A5WMP8.
ModBase	Search...
Protein-protein interaction databases
STRING	A5WMP8.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5222273.
GenomeReviews	Gene locus TBFG_11594 in contig CP000717_GR.
KEGG	mtf:TBFG_11594.
PATRIC	18134184. VBIMycTub9078_1745.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	EGFVYQG.
ProtClustDB	CLSK799816.
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR006047. Glyco_hydro_13_cat_dom. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR022567. Maltooligo_trehalose_bac_C. IPR012768. Trehalose_TreZ. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K01236.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF21. PTHR10357:SF21. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF11941. DUF3459. 1 hit. [Graphical view]
PIRSF	PIRSF006337. Trehalose_TreZ. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR02402. Trehalose_TreZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A5WMP8_MYCTF

Accession

Primary (citable) accession number: A5WMP8

Entry history

Integrated into UniProtKB/TrEMBL:	July 28, 2009
Last sequence update:	July 28, 2009
Last modified:	December 14, 2011
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)