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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Mycobacterium tuberculosis (strain F11)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei531D-inositol 3-phosphateUniRule annotation1
Binding sitei67UDP-GlcNAc; via amide nitrogenUniRule annotation1
Binding sitei1221D-inositol 3-phosphateUniRule annotation1
Binding sitei1551D-inositol 3-phosphateUniRule annotation1
Binding sitei1791D-inositol 3-phosphateUniRule annotation1
Binding sitei1991D-inositol 3-phosphateUniRule annotation1
Binding sitei273UDP-GlcNAcUniRule annotation1
Binding sitei278UDP-GlcNAcUniRule annotation1
Binding sitei331UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi340Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi341Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi343Magnesium; via carbonyl oxygenUniRule annotation1
Binding sitei353UDP-GlcNAcUniRule annotation1
Binding sitei361UDP-GlcNAcUniRule annotation1
Metal bindingi367MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:TBFG_10494
OrganismiMycobacterium tuberculosis (strain F11)
Taxonomic identifieri336982 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004001421 – 480D-inositol 3-phosphate glycosyltransferaseAdd BLAST480

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA5WJJ8.
SMRiA5WJJ8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni59 – 60UDP-GlcNAc bindingUniRule annotation2
Regioni64 – 691D-inositol 3-phosphate bindingUniRule annotation6

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

A5WJJ8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGVRHDDGS GLIAQRRPVR GEGATRSRGP SGPSNRNVSA ADDPRRVALL
60 70 80 90 100
AVHTSPLAQP GTGDAGGMNV YMLQSALHLA RRGIEVEIFT RATASADPPV
110 120 130 140 150
VRVAPGVLVR NVVAGPFEGL DKYDLPTQLC AFAAGVLRAE AVHEPGYYDI
160 170 180 190 200
VHSHYWLSGQ VGWLARDRWA VPLVHTAHTL AAVKNAALAD GDGPEPPLRT
210 220 230 240 250
VGEQQVVDEA DRLIVNTDDE ARQVISLHGA DPARIDVVHP GVDLDVFRPG
260 270 280 290 300
DRRAARAALG LPVDERVVAF VGRIQPLKAP DIVLRAAAKL PGVRIIVAGG
310 320 330 340 350
PSGSGLASPD GLVRLADELG ISARVTFLPP QSHTDLATLF RAADLVAVPS
360 370 380 390 400
YSESFGLVAV EAQACGTPVV AAAVGGLPVA VRDGITGTLV SGHEVGQWAD
410 420 430 440 450
AIDHLLRLCA GPRGRVMSRA AARHAATFSW ENTTDALLAS YRRAIGEYNA
460 470 480
ERQRRGGEVI SDLVAVGKPR HWTPRRGVGA
Length:480
Mass (Da):50,541
Last modified:July 28, 2009 - v1
Checksum:i2134755E894A9CCF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000717 Genomic DNA. Translation: ABR04837.1.

Genome annotation databases

EnsemblBacteriaiABR04837; ABR04837; TBFG_10494.
KEGGimtf:TBFG_10494.
PATRICi18131721. VBIMycTub9078_0534.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000717 Genomic DNA. Translation: ABR04837.1.

3D structure databases

ProteinModelPortaliA5WJJ8.
SMRiA5WJJ8.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR04837; ABR04837; TBFG_10494.
KEGGimtf:TBFG_10494.
PATRICi18131721. VBIMycTub9078_0534.

Phylogenomic databases

HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHA_MYCTF
AccessioniPrimary (citable) accession number: A5WJJ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: July 28, 2009
Last modified: November 2, 2016
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.