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A5WHT0

- GLMU_PSYWF

UniProt

A5WHT0 - GLMU_PSYWF

Protein

Bifunctional protein GlmU

Gene

glmU

Organism
Psychrobacter sp. (strain PRwf-1)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 55 (01 Oct 2014)
      Sequence version 1 (10 Jul 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetylglucosamine (UDP-GlcNAc). The C-terminal domain catalyzes the transfer of acetyl group from acetyl coenzyme A to glucosamine-1-phosphate (GlcN-1-P) to produce N-acetylglucosamine-1-phosphate (GlcNAc-1-P), which is converted into UDP-GlcNAc by the transfer of uridine 5-monophosphate (from uridine 5-triphosphate), a reaction catalyzed by the N-terminal domain.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate.UniRule annotation
    UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei24 – 241UDP-GlcNAcUniRule annotation
    Binding sitei75 – 751UDP-GlcNAcUniRule annotation
    Metal bindingi104 – 1041MagnesiumUniRule annotation
    Binding sitei138 – 1381UDP-GlcNAc; via amide nitrogenUniRule annotation
    Binding sitei153 – 1531UDP-GlcNAcUniRule annotation
    Binding sitei168 – 1681UDP-GlcNAcUniRule annotation
    Metal bindingi226 – 2261MagnesiumUniRule annotation
    Binding sitei226 – 2261UDP-GlcNAcUniRule annotation
    Binding sitei332 – 3321Acetyl-CoA; amide nitrogenUniRule annotation
    Binding sitei350 – 3501Acetyl-CoAUniRule annotation
    Active sitei362 – 3621Proton acceptorUniRule annotation
    Binding sitei365 – 3651Acetyl-CoAUniRule annotation
    Binding sitei376 – 3761Acetyl-CoAUniRule annotation
    Binding sitei422 – 4221Acetyl-CoA; via amide nitrogenUniRule annotation
    Binding sitei439 – 4391Acetyl-CoAUniRule annotation

    GO - Molecular functioni

    1. glucosamine-1-phosphate N-acetyltransferase activity Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. UDP-N-acetylglucosamine diphosphorylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cell morphogenesis Source: UniProtKB-HAMAP
    2. lipid A biosynthetic process Source: UniProtKB-UniPathway
    3. lipopolysaccharide biosynthetic process Source: InterPro
    4. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
    5. regulation of cell shape Source: UniProtKB-KW
    6. UDP-N-acetylglucosamine biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Acyltransferase, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciPPRW349106:GHZF-2351-MONOMER.
    UniPathwayiUPA00113; UER00532.
    UPA00113; UER00533.
    UPA00973.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional protein GlmUUniRule annotation
    Including the following 2 domains:
    UDP-N-acetylglucosamine pyrophosphorylaseUniRule annotation (EC:2.7.7.23UniRule annotation)
    Alternative name(s):
    N-acetylglucosamine-1-phosphate uridyltransferaseUniRule annotation
    Glucosamine-1-phosphate N-acetyltransferaseUniRule annotation (EC:2.3.1.157UniRule annotation)
    Gene namesi
    Name:glmUUniRule annotation
    Ordered Locus Names:PsycPRwf_2281
    OrganismiPsychrobacter sp. (strain PRwf-1)
    Taxonomic identifieri349106 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter
    ProteomesiUP000001993: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 455455Bifunctional protein GlmUPRO_1000073649Add
    BLAST

    Interactioni

    Subunit structurei

    Homotrimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi349106.PsycPRwf_2281.

    Structurei

    3D structure databases

    ProteinModelPortaliA5WHT0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 228228PyrophosphorylaseUniRule annotationAdd
    BLAST
    Regioni10 – 134UDP-GlcNAc bindingUniRule annotation
    Regioni80 – 812UDP-GlcNAc bindingUniRule annotation
    Regioni102 – 1043UDP-GlcNAc bindingUniRule annotation
    Regioni229 – 24921LinkerUniRule annotationAdd
    BLAST
    Regioni250 – 455206N-acetyltransferaseUniRule annotationAdd
    BLAST
    Regioni385 – 3862Acetyl-CoA bindingUniRule annotation

    Sequence similaritiesi

    In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.UniRule annotation
    In the C-terminal section; belongs to the transferase hexapeptide repeat family.UniRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1207.
    HOGENOMiHOG000283476.
    KOiK04042.
    OMAiDCVTNQD.
    OrthoDBiEOG6Z6FQZ.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    HAMAPiMF_01631. GlmU.
    InterProiIPR005882. Bifunctional_GlmU.
    IPR001451. Hexapep_transf.
    IPR018357. Hexapep_transf_CS.
    IPR025877. MobA-like_NTP_Trfase_dom.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR011004. Trimer_LpxA-like.
    [Graphical view]
    PfamiPF00132. Hexapep. 4 hits.
    PF12804. NTP_transf_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF51161. SSF51161. 1 hit.
    SSF53448. SSF53448. 1 hit.
    TIGRFAMsiTIGR01173. glmU. 1 hit.
    PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A5WHT0-1 [UniParc]FASTAAdd to Basket

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    MNNTLTTIIL AAGKGTRMQS AKPKVLQILA DKPLLAHVLD TCQSISVDKT    50
    IVVYGFGGDQ VQQAMTDYSL TWVEQTEQLG TGHAVKVALD ELPSTGKSLI 100
    LYGDVPLVSA ETLSRLKQAN VQGMSMLTLT VDNPFGLGRI KRDEQGNITA 150
    IVEQKDASEQ EQAIREINSG IYCVDNALLH QYLPNLSNDN AQQEYYLTDI 200
    VKMAVADGIA IAAIEPDYEF EIEGVNNRQQ LAQLERKWQA KLVEDLQVQG 250
    VQFADPNRVD IRGEVSVGQD VFVDINVVFK GKVSLGNNVT IEAGCMIKDS 300
    QIGDNVHIKP YCVFDDAQVA QGATIGPFAH LRPQTVLEKN TRLGNFVEIK 350
    KSRIGEGSKV NHLSYVGDAQ IGAGVNFGAG AITCNYDGVN KHQTIVGDNA 400
    FIGTNTSLVA PVTIGQTATI GAGSVITKNV EDNALAIGRG RQVQKDNYQR 450
    PEKKK 455
    Length:455
    Mass (Da):49,296
    Last modified:July 10, 2007 - v1
    Checksum:i6074BD4D1C91F6F6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000713 Genomic DNA. Translation: ABQ95221.1.
    RefSeqiWP_011961493.1. NC_009524.1.
    YP_001281171.1. NC_009524.1.

    Genome annotation databases

    EnsemblBacteriaiABQ95221; ABQ95221; PsycPRwf_2281.
    GeneIDi5205575.
    KEGGiprw:PsycPRwf_2281.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000713 Genomic DNA. Translation: ABQ95221.1 .
    RefSeqi WP_011961493.1. NC_009524.1.
    YP_001281171.1. NC_009524.1.

    3D structure databases

    ProteinModelPortali A5WHT0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 349106.PsycPRwf_2281.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABQ95221 ; ABQ95221 ; PsycPRwf_2281 .
    GeneIDi 5205575.
    KEGGi prw:PsycPRwf_2281.

    Phylogenomic databases

    eggNOGi COG1207.
    HOGENOMi HOG000283476.
    KOi K04042.
    OMAi DCVTNQD.
    OrthoDBi EOG6Z6FQZ.

    Enzyme and pathway databases

    UniPathwayi UPA00113 ; UER00532 .
    UPA00113 ; UER00533 .
    UPA00973 .
    BioCyci PPRW349106:GHZF-2351-MONOMER.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    HAMAPi MF_01631. GlmU.
    InterProi IPR005882. Bifunctional_GlmU.
    IPR001451. Hexapep_transf.
    IPR018357. Hexapep_transf_CS.
    IPR025877. MobA-like_NTP_Trfase_dom.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR011004. Trimer_LpxA-like.
    [Graphical view ]
    Pfami PF00132. Hexapep. 4 hits.
    PF12804. NTP_transf_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51161. SSF51161. 1 hit.
    SSF53448. SSF53448. 1 hit.
    TIGRFAMsi TIGR01173. glmU. 1 hit.
    PROSITEi PS00101. HEXAPEP_TRANSFERASES. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: PRwf-1.

    Entry informationi

    Entry nameiGLMU_PSYWF
    AccessioniPrimary (citable) accession number: A5WHT0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 55 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3